Dimethyl tetrasulfide
Based on 1 Customer Validation
Dimethyl tetrasulfide (1,4-Dimethyltetrasulfane) is an apoptosis inducer and a DNA fragmentation inducer. Dimethyl tetrasulfide targets caspase-3. Dimethyl tetrasulfide induces the production of ROS. Dimethyl tetrasulfide is cytotoxic to leukemia cells. Dimethyl tetrasulfide can be used in the study of leukemia.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 5756-24-1
- Formula: C2H6S4
- Molecular Weight:158.33
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jurkat T cells
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Concentration:5 mM; 20 mM
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Incubation Time:24 h; 48 h
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Result:Unaffected viability or slightly reduced after 24 or 48 h at 5 mM.
Reduced viability approximately 60% at 20 mM.
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Cell Line:HL-60 cells
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Concentration:5 mM; 20 mM
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Incubation Time:24 h; 48 h
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Result:Reduced HL-60 viability; at 20 mM for 48 h, viability was 28%.
Chemical Information
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CAS No. 5756-24-1
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Appearance Liquid (Density: 1.348±0.06 g/cm3)
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Molecular Weight 158.33
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Formula C2H6S4
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Color Colorless to light yellow
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SMILES
CSSSSC
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Synonyms
1,4-Dimethyltetrasulfane
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (631.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Moon HI, et al. Larvicidal activity of major essential oils from stems of Allium monanthum Maxim. against Aedes aegypti L. Journal of enzyme inhibition and medicinal chemistry. 2011 Dec;26(6):827-30. [Content Brief]
[3]. Zhang G, et al. Effect of dimethyl sulfides on the induction of apoptosis in human leukemia Jurkat cells and HL-60 cells. Bioscience, biotechnology, and biochemistry. 2008 Nov;72(11):2966-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.3159 mL | 31.5796 mL | 63.1592 mL | 157.8981 mL |
| 5 mM | 1.2632 mL | 6.3159 mL | 12.6318 mL | 31.5796 mL | |
| 10 mM | 0.6316 mL | 3.1580 mL | 6.3159 mL | 15.7898 mL | |
| 15 mM | 0.4211 mL | 2.1053 mL | 4.2106 mL | 10.5265 mL | |
| 20 mM | 0.3158 mL | 1.5790 mL | 3.1580 mL | 7.8949 mL | |
| 25 mM | 0.2526 mL | 1.2632 mL | 2.5264 mL | 6.3159 mL | |
| 30 mM | 0.2105 mL | 1.0527 mL | 2.1053 mL | 5.2633 mL | |
| 40 mM | 0.1579 mL | 0.7895 mL | 1.5790 mL | 3.9475 mL | |
| 50 mM | 0.1263 mL | 0.6316 mL | 1.2632 mL | 3.1580 mL | |
| 60 mM | 0.1053 mL | 0.5263 mL | 1.0527 mL | 2.6316 mL | |
| 80 mM | 0.0789 mL | 0.3947 mL | 0.7895 mL | 1.9737 mL | |
| 100 mM | 0.0632 mL | 0.3158 mL | 0.6316 mL | 1.5790 mL |
Dimethyl tetrasulfide Related Classifications
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