Celastramycin A
Based on 1 Customer Validation
Celastramycin A is a potent innate immune suppressor and Antibacterial agent. Celastramycin A inhibits TNF-α-stimulated IL-8 production and reduces IL-6 in a ZFC3H1-independent manner. Celastramycin A inhibits the growth of Pasteurella multocida and Mannheimia haemolytica and modulates the immune system. Celastramycin A can be used for research on bovine respiratory disease (BRD).
For research use only. We do not sell to patients.
- Purity : 98.23%
- CAS No.: 188988-62-7
- Formula: C17H18Cl3NO3
- Molecular Weight:390.69
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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IL-8 |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HUVEC | IC50 |
0.06 μg/mL
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Suppression of TNF-α-stimulated IL-8 production in human umbilical vein endothelial cells (HUVECs).
Suppression of TNF-α-stimulated IL-8 production in human umbilical vein endothelial cells (HUVECs).
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19317429 |
| HeLa | CC50 |
16.9 mg/L
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Cytotoxicity against HeLa cells with a reported CC50 of 16.9 mg/L.
Cytotoxicity against HeLa cells with a reported CC50 of 16.9 mg/L.
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38391521 |
In Vitro
Celastramycin A (0.1 μg/mL) reduces TNFα-induced IL-8 and CCL2 expression by approximately 50% in LU99 cells, and the reduction of IL-8 is reversed by ZFC3H1 knockdown[2].
Celastramycin A decreases IL-8 promoter-driven Firefly luciferase activity in LU99 cells, suggesting that it inhibits proximal IL-8 promoter activation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LU99 cells
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Concentration:1 μg/mL; 0.01-0.1 μg/mL
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Incubation Time:6 h
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Result:Incubation with 1 mg/mL Celastramycin A for 6 h reduced LU99 viability by 10%.
No significant loss was seen at 0.01-0.1 mg/mL.
Chemical Information
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CAS No. 188988-62-7
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Appearance Solid
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Molecular Weight 390.69
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Formula C17H18Cl3NO3
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Color Off-white to light yellow
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SMILES
O=C(C1=C(O)C(CCCCCC)=CC(Cl)=C1O)C2=CC(Cl)=C(Cl)N2
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Structure Classification
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Initial Source
Drosophila
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (255.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5596 mL | 12.7979 mL | 25.5957 mL | 63.9894 mL |
| 5 mM | 0.5119 mL | 2.5596 mL | 5.1191 mL | 12.7979 mL | |
| 10 mM | 0.2560 mL | 1.2798 mL | 2.5596 mL | 6.3989 mL | |
| 15 mM | 0.1706 mL | 0.8532 mL | 1.7064 mL | 4.2660 mL | |
| 20 mM | 0.1280 mL | 0.6399 mL | 1.2798 mL | 3.1995 mL | |
| 25 mM | 0.1024 mL | 0.5119 mL | 1.0238 mL | 2.5596 mL | |
| 30 mM | 0.0853 mL | 0.4266 mL | 0.8532 mL | 2.1330 mL | |
| 40 mM | 0.0640 mL | 0.3199 mL | 0.6399 mL | 1.5997 mL | |
| 50 mM | 0.0512 mL | 0.2560 mL | 0.5119 mL | 1.2798 mL | |
| 60 mM | 0.0427 mL | 0.2133 mL | 0.4266 mL | 1.0665 mL | |
| 80 mM | 0.0320 mL | 0.1600 mL | 0.3199 mL | 0.7999 mL | |
| 100 mM | 0.0256 mL | 0.1280 mL | 0.2560 mL | 0.6399 mL |
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