2. Anti-infection
    Metabolic Enzyme/Protease
  3. Bacterial
    Fungal
    Aldehyde Dehydrogenase (ALDH)
  4. Dyclonine hydrochloride

Dyclonine hydrochloride  (Synonyms: Dyclocaine hydrochloride)

Cat. No.: HY-B0364A Purity: 99.86%
COA Handling Instructions

Dyclonine (Dyclocaine) hydrochloride is an orally effective ALDH covalent inhibitor (crosses blood-brain barrier), with an IC50 of 35 µM for ALDH2 and 76 µM for ALDH3A1. Dyclonine hydrochloride has sensitizing activities for targeted cancer cells and antibacterial. Dyclonine hydrochloride is also a local anesthetic that blocks the transmission of various nerve impulses or stimuli and inhibits the sensation of touch and pain.

For research use only. We do not sell to patients.

Dyclonine hydrochloride Chemical Structure

Dyclonine hydrochloride Chemical Structure

CAS No. : 536-43-6

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10 mM * 1 mL in DMSO USD 61 In-stock
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10 g USD 92 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Dyclonine hydrochloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dyclonine (Dyclocaine) hydrochloride is an orally effective ALDH covalent inhibitor (crosses blood-brain barrier), with an IC50 of 35 µM for ALDH2 and 76 µM for ALDH3A1. Dyclonine hydrochloride has sensitizing activities for targeted cancer cells and antibacterial. Dyclonine hydrochloride is also a local anesthetic that blocks the transmission of various nerve impulses or stimuli and inhibits the sensation of touch and pain[1][2][3].

IC50 & Target[3]

ALDH2

35 μM (IC50)

ALDH3

76 μM (IC50)

In Vitro

Dyclonine hydrochloride (50 µM; 24 h) sensitizes cancer cells to deficiency of cysteine and GSH[1].
Dyclonine hydrochloride (0-2048 µg/mL; 24 or 48 h) shows significant bactericidal and fungicidal activity[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HSC-4 cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Inhibited ALDH activity and induced 4-HNE accumulation in GSH-depleted cancer cells.

Cell Viability Assay[2]

Cell Line: S. aureus, B. subtilis, E. coli, T. mentagrophyte
Concentration: 0-2048 µg/mL
Incubation Time: 24 h (for bacterial), 48 h (for fungi)
Result: Showed good antibacterial and antifungal activity, with minimum microbicidal concentration of 0.006%, 0.025%, 0.012% and 0.025% for S. aureus, B. subtilis, E. coli, T. mentagrophyte, separately.
In Vivo

Dyclonine hydrochloride (5 mg/kg; i.p.; single daily for 24 days) sensitizes the involucrin+ differentiated tumor cells to sulfasalazine treatment in vivo[1].
Dyclonine hydrochloride (5 mg/kg; i.p.; single daily for 21 days) suppress the growth of tumors formed by ALDH3A1-expressing gastric cancer stemlike cells when combines with sulfasalazine[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mice (HSC-2 cells implanted model)[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; single daily for 24 days (combine with sulfasalazine).
Result: Attenuated the formation of tumors by HSC-2 cells implanted in nude mice when combined with sulfasalazine.
Animal Model: C57BL6 mice (K19-Wnt1/C2mE-KP cells implanted model)[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; single daily for 21 days (combine with sulfasalazine).
Result: Enhanced the antitumor effect of sulfasalazine on HNSCC tumors consisting of CD44vhigh stemlike tumor cells and involucrin+ differentiated tumor cells.
Molecular Weight

325.87

Appearance

Solid

Formula

C18H28ClNO2
CAS No.
SMILES

O=C(C1=CC=C(OCCCC)C=C1)CCN2CCCCC2.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (153.44 mM; Need ultrasonic)

DMSO : ≥ 25 mg/mL (76.72 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0687 mL 15.3435 mL 30.6871 mL
5 mM 0.6137 mL 3.0687 mL 6.1374 mL
10 mM 0.3069 mL 1.5344 mL 3.0687 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.86%

COA (243 KB) RP-HPLC (244 KB) MS (68 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Dyclonine536-43-6DyclocaineBacterialFungalAldehyde Dehydrogenase (ALDH)HSC-4 cellsS. aureusB. subtilisE. coliT. mentagrophyteanestheticcancerInhibitorinhibitorinhibit

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