2. Epigenetics Apoptosis
  3. Histone Methyltransferase Apoptosis
  4. EI1

EI1  (Synonyms: KB-145943)

Cat. No.: HY-15573 Purity: 99.78%
COA Handling Instructions

EI1 (KB-145943) is a potent and selective EZH2 inhibitor with IC50 of 15 nM and 13 nM for EZH2 (WT) and EZH2 (Y641F), respectively.

For research use only. We do not sell to patients.

EI1 Chemical Structure

EI1 Chemical Structure

CAS No. : 1418308-27-6

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 90 In-stock
Solution
10 mM * 1 mL in DMSO USD 90 In-stock
Solid
5 mg USD 105 In-stock
10 mg USD 176 In-stock
50 mg USD 528 In-stock
100 mg USD 765 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

EI1 Related Antibodies

Top Publications Citing Use of Products

View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms
EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D SETDB1/KMT2G SETD2/KMT3A SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

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  • References

  • Customer Review

Description

EI1 (KB-145943) is a potent and selective EZH2 inhibitor with IC50 of 15 nM and 13 nM for EZH2 (WT) and EZH2 (Y641F), respectively.

IC50 & Target[1]

EZH2 Y641F mutant type

13 nM (IC50)

EZH2 WT

15 nM (EC50)

In Vitro

In DLBCL cells, EI1 (KB-145943) inhibits cellular H3K27 methylation and activates Ezh2 target gene p16 expression. In mouse embryonic fibroblasts, EI1 (KB-145943) also inhibit H3K27me3 and cell proliferation. In addition, EI1 (KB-145943) selectively inhibits the growth of DLBCL cells carrying Ezh2 mutation, and causes cell cycle arrest and apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EI1 Related Antibodies
Molecular Weight

390.48

Appearance

Solid

Formula

C23H26N4O2
CAS No.
SMILES

CCC(CC)N(C=C1)C2=C1C(C(NCC3=C(C)C=C(C)NC3=O)=O)=CC(C#N)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 7.69 mg/mL (19.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5610 mL 12.8048 mL 25.6095 mL
5 mM 0.5122 mL 2.5610 mL 5.1219 mL
10 mM 0.2561 mL 1.2805 mL 2.5610 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
Cell Assay
[1]

Exponentially growing diffused large B-cell lymphoma (DLBCL) cells are seeded in 12-well plates at a density of 1×105 cells/mL with the indicated concentration of EI1 (KB-145943). Viable cell number is determined every 3-4 d for up to 14 or 15 d by Vi-CELL. Mouse embryonic fibroblasts (MEFs) are seeded in a six-well plate at 2.5×104 cell/mL and treated with EI1 (KB-145943) (3.3 μM) or 4-OH-tamoxifen (100 nM). Viable cell number is determined at days 3, 6 and 11. On days of cell counts, fresh growth medium and compound are replenwashed and cells split back to a density of 1×105 cells/mL. Total cell number is expressed as split-adjusted viable cells per milliliter. IC50 is calculated by PRISM and all proliferation experiments are repeated more than two times and representative data are presented.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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EI1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

EI11418308-27-6KB-145943EI 1EI-1KB145943KB 145943Histone MethyltransferaseApoptosisInhibitorinhibitorinhibit

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