MSOP

Rats^[2]
Male Sprague-Dawley rats (250-300 g) are housed individually in cages on a standard 12 h-12 h light-dark cycle. Water and food are available as libitum until rats are transported to the labotatory approximately 1 h before the experiments. A glutamate transporter activator, three glutamate transporter inhibitors, TBOA, DL-THA, dihydrokainate, and a selective group III mGluR antagonist MSOP are used. All drugs are dissolved in 0.9% physiological saline. To examine the role of group III mGluRs in the antinociceptive effect produced by intrathecal TBOA in the formalin model, the rats are intrathecally injected with saline (10 μL; n=5), MSOP (10 μg/10 μL; n=5), TBOA (10 μg/10 μL; n=5), or MSOP plus TBOA (n=5). Ten minutes later, 2% formalin (100 μL) is injected into the plantar side of a hind paw and formalin-induced pain behaviors are assessed.To examine the role of group III mGluRs in the antinociceptive effect produced by intrathecal TBOA in the complete Freund’s adjuvant (CFA) model, the rats are intrathecally injected with saline (10 μL; n=5), MSOP (10 μg/10 μL; n=5), TBOA (10 μg/10 μL; n=5), or MSOP plus TBOA (n=5) at 6 h post-CFA and then measured paw withdrawal latencies^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.