2-Hydroxycinnamaldehyde
Based on 1 Customer Validation
2-Hydroxycinnamaldehyde is a phenylpropanoid that can be isolated from the bark of Cinnamomum cassia. 2-Hydroxycinnamaldehyde inhibits Wnt/β-catenin, STAT3 signaling. 2-Hydroxycinnamaldehyde induces cell apoptosis 2-Hydroxycinnamaldehyde has antitumor and anti-inflammation activities.
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- Pureza : 99.86%
- No. CAS: 3541-42-2
- Fòrmula: C9H8O2
- Peso molecular:148.16
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Almacenamiento:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
6.17 μM
Compound: 1
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Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 23855266] |
| HCT-116 | IC50 |
6.62 μM
Compound: 1
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Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by SRB assay
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[PMID: 23855266] |
| HCT-116 | IC50 |
8.14 μM
Compound: 1
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Cytotoxicity against human HCT116 cells assessed as growth inhibition after 24 hrs by SRB assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 24 hrs by SRB assay
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[PMID: 23855266] |
| SW-620 | GI50 |
15 μM
Compound: 1, HCA
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Cytotoxicity against human SW620 cells after 48 hrs by WST1 assay
Cytotoxicity against human SW620 cells after 48 hrs by WST1 assay
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[PMID: 21183341] |
Chemical Information
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No. CAS 3541-42-2
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Appearance Solid
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Peso molecular 148.16
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Fòrmula C9H8O2
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Color Light yellow to brown
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SMILES
O=CC=CC1=C(O)C=CC=C1
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (674.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (16.87 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (299 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Lee MA, et al. Antitumor activity of 2-hydroxycinnamaldehyde for human colon cancer cells through suppression of β-catenin signaling. J Nat Prod. 2013 Jul 26;76(7):1278-84. [Content Brief]
[2]. Yoon YJ, et al. 2'-hydroxycinnamaldehyde inhibits cancer cell proliferation and tumor growth by targeting the pyruvate kinase M2. Cancer Lett. 2018 Oct 10;434:42-55. [Content Brief]
[3]. Hao Let al. 2'-Hydroxycinnamaldehyde ameliorates imiquimod-induced psoriasiform inflammation by targeting PKM2-STAT3 signaling in mice. Exp Mol Med. 2021 May;53(5):875-884. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.7495 mL | 33.7473 mL | 67.4946 mL | 168.7365 mL |
| 5 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL | 33.7473 mL | |
| 10 mM | 0.6749 mL | 3.3747 mL | 6.7495 mL | 16.8736 mL | |
| 15 mM | 0.4500 mL | 2.2498 mL | 4.4996 mL | 11.2491 mL | |
| 20 mM | 0.3375 mL | 1.6874 mL | 3.3747 mL | 8.4368 mL | |
| 25 mM | 0.2700 mL | 1.3499 mL | 2.6998 mL | 6.7495 mL | |
| 30 mM | 0.2250 mL | 1.1249 mL | 2.2498 mL | 5.6245 mL | |
| 40 mM | 0.1687 mL | 0.8437 mL | 1.6874 mL | 4.2184 mL | |
| 50 mM | 0.1350 mL | 0.6749 mL | 1.3499 mL | 3.3747 mL | |
| 60 mM | 0.1125 mL | 0.5625 mL | 1.1249 mL | 2.8123 mL | |
| 80 mM | 0.0844 mL | 0.4218 mL | 0.8437 mL | 2.1092 mL | |
| 100 mM | 0.0675 mL | 0.3375 mL | 0.6749 mL | 1.6874 mL |
2-Hydroxycinnamaldehyde Related Classifications
HY-N3149A Related Classifications
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