Pleuromutilin
Based on 1 Customer Validation
Pleuromutilin (Drosophilin B) exhibits antibacterial activity through inhibition of protein synthesis.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.0%
- No. CAS: 125-65-5
- Fòrmula: C22H34O5
- Peso molecular:378.50
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Ver todos los productos específicos de isoformas Antibiotic
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 4T1 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against mouse 4T1 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against mouse 4T1 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
| A-375 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against human A-375 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against human A-375 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
| B16-F10 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against mouse B16-F10 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against mouse B16-F10 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
| CT26 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against mouse CT26 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against mouse CT26 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
| HCT-116 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
| Kasumi 1 | IC50 |
>100 μM
Compound: Pleuromutilin
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Cytotoxicity against human Kasumi 1 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
Cytotoxicity against human Kasumi 1 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
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[PMID: 34704758] |
Pleuromutilin exhibits board-spectrum antibacterial activity against Gram-positive bacteria (such as MRSA, S. pyogenes, S. pneumoniae), some fastidious Gram-negative bacteria (such as H. influenzae and M. catarrhalis), anaerobic bacteria (such as C. difficile, P. acnes), atypical pathogens (such as M. pneumoniae, C. pneumoniae), and mycobacteria (such as M. tuberculosis)[2].
Pleuromutilin binds to the peptidyl transferase center (PTC) of the 50S subunit of the bacterial ribosome, induces conformational changes in ribosomal RNA, interfers with the translocation of tRNA, blocks the translation process, thereby inhibiting the bacterial protein synthesis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 125-65-5
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Appearance Solid
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Peso molecular 378.50
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Fòrmula C22H34O5
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Color White to off-white
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SMILES
C[C@@H]([C@@H]([C@@](C)(C[C@H]1OC(CO)=O)C=C)O)C(CC[C@H]2C)(CCC3=O)[C@]3([H])C21C
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Synonyms
Drosophilin B; Mutilin 14-glycolate
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Structure Classification
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Initial Source
fungus, Pleurotus mutilus
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 200 mg/mL (528.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6420 mL | 13.2100 mL | 26.4201 mL | 66.0502 mL |
| 5 mM | 0.5284 mL | 2.6420 mL | 5.2840 mL | 13.2100 mL | |
| 10 mM | 0.2642 mL | 1.3210 mL | 2.6420 mL | 6.6050 mL | |
| 15 mM | 0.1761 mL | 0.8807 mL | 1.7613 mL | 4.4033 mL | |
| 20 mM | 0.1321 mL | 0.6605 mL | 1.3210 mL | 3.3025 mL | |
| 25 mM | 0.1057 mL | 0.5284 mL | 1.0568 mL | 2.6420 mL | |
| 30 mM | 0.0881 mL | 0.4403 mL | 0.8807 mL | 2.2017 mL | |
| 40 mM | 0.0661 mL | 0.3303 mL | 0.6605 mL | 1.6513 mL | |
| 50 mM | 0.0528 mL | 0.2642 mL | 0.5284 mL | 1.3210 mL | |
| 60 mM | 0.0440 mL | 0.2202 mL | 0.4403 mL | 1.1008 mL | |
| 80 mM | 0.0330 mL | 0.1651 mL | 0.3303 mL | 0.8256 mL | |
| 100 mM | 0.0264 mL | 0.1321 mL | 0.2642 mL | 0.6605 mL |
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