Kasugamycin
Based on 1 publication(s) in Google Scholar
Kasugamycin (Ksg) is an aminoglycoside antibiotic (antibiotic) that binds to the bacterial 30S ribosomal subunit and inhibits canonical translation initiation. Kasugamycin binds to the P-site and E-site codon regions in the mRNA channel and interferes with mRNA-tRNA codon-anticodon interactions, thereby destabilizing initiator tRNA binding. Kasugamycin possesses anti-infective activity. Kasugamycin is used in research on bacterial translation and Pseudomonas infection.
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- Pureza : 99.84%
- No. CAS: 6980-18-3
- Fòrmula: C14H25N3O9
- Peso molecular:379.36
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Kasugamycin
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Actividad biológica
Descripciòn
In Vitro
Kasugamycin (Ksg) (100 mM) binds to the Thermus thermophilus 30S ribosomal subunit, forming two binding sites in the mRNA pathway; the primary binding site is located at the top of h44, between h24 and h28, and spans the P/E site region, while the secondary binding site is located adjacent to the E site and interacts with h23, h24 of 16S rRNA and ribosomal protein S7, with the Kasugamycin-30S complex structure determined at a resolution of 3.35 Å[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) inhibits the binding of MVF-mRNA to Escherichia coli 30S ribosomal subunits and 70S ribosomes, with inhibition rates of 40% and 25% at 1 mM, respectively[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) inhibits the binding of initiator fMet-tRNA to MVF-mRNA-programmed Escherichia coli 30S ribosomal subunits, with a maximum inhibition rate of 75% at 1 mM, while it has no significant effect on the binding of fMet-tRNA to 70S ribosomes[2].
Kasugamycin (500 μM; 15 min; 37 °C) inhibits the binding of leaderless mRNA and initiator fMet-tRNA to Escherichia coli 70S ribosomes by 29% and 13%, respectively, indicating that the 70S initiation complex formed by leaderless mRNA-fMet-tRNA is relatively insensitive to Kasugamycin[2].
Kasugamycin (12.5-50 μg/mL) inhibits 32 strains of Pseudomonas aeruginosa cultured in peptone water at pH 7.0, with 1 strain inhibited at 12.5 μg/mL, 19 strains inhibited at 25 μg/mL, and 12 strains inhibited at 50 μg/mL[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Kasugamycin (100 mg/kg; i.p.; single administration) achieves a 50% survival rate in mice infected intraperitoneally with mucin-containing Pseudomonas aeruginosa No. 87 (100 MLD)[4].
Kasugamycin (200 mg/kg; i.p.; single administration) achieves a 75% survival rate in mice infected intraperitoneally with mucin-containing Pseudomonas aeruginosa No. 87 (100 MLD)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:200 mg/kg
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Administration:i.p.; single injection
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Result:Resulted in 100% survival.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:100 mg/kg
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Administration:i.p.; single administration
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Result:Resulted in 50% survival.
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Animal Model:Mice were intraperitoneally inoculated with Pseudomonas aeruginosa No. 87 with mucin at 1 MLD.[4]
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Dosage:200 mg/kg
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Administration:i.p.; single administration
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Result:Resulted in 75% survival.
Chemical Information
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No. CAS 6980-18-3
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Appearance Solid
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Peso molecular 379.36
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Fòrmula C14H25N3O9
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Color White to off-white
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SMILES
OC(C(N[C@@H](C[C@@H]1N)[C@@H](C)O[C@]1([H])O[C@@H]2[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H]2O)=N)=O
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Synonyms
Ksg
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Front Mol Biosci
Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases. [Abstract]2021 Apr 7:8:640356. PMID: 33898519
Solvente y solubilidad
In Vitro:
H2O : 7 mg/mL (18.45 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (290 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Zhang H, et al. Simultaneous determination of kasugamycin and validamycin-A residues in cereals by consecutive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment. 2018 Mar;35(3):487-497. [Content Brief]
[2]. Schluenzen F, et al. The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation. Nature structural & molecular biology. 2006 Oct;13(10):871-8. [Content Brief]
[3]. Kasuga K, et al. Heterologous production of kasugamycin, an aminoglycoside antibiotic from Streptomyces kasugaensis, in Streptomyces lividans and Rhodococcus erythropolis L-88 by constitutive expression of the biosynthetic gene cluster. Applied microbiology and biotechnology. 2017 May;101(10):4259-4268. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6360 mL | 13.1801 mL | 26.3602 mL | 65.9005 mL |
| 5 mM | 0.5272 mL | 2.6360 mL | 5.2720 mL | 13.1801 mL | |
| 10 mM | 0.2636 mL | 1.3180 mL | 2.6360 mL | 6.5900 mL | |
| 15 mM | 0.1757 mL | 0.8787 mL | 1.7573 mL | 4.3934 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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