Mellein
Based on 1 publication(s) in Google Scholar
Mellein ((R)-Mellein) is an isocoumarin compound with antibacterial activity. Mellein inhibits the activity of HCV Protease, disrupts the cell wall of Xanthomonas sp., and acts as an insect trail pheromone. Mellein is applicable to research related to fungal infections, bacterial infections, and hepatitis C virus infections.
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- Pureza: 98.54%
- No. CAS: 480-33-1
- Fòrmula: C10H10O3
- Peso molecular:178.18
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Antifungal Compound Library
HY-L048
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Antibacterial Compound Library
HY-L049
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Phenols Library
HY-L057
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Antibiotics Library
HY-L067
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Anti-Hepatitis C Virus Compound Library
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Microbial Metabolite Library
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Publications Citing Use of MedChemExpress (MCE) Mellein
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Actividad biológica
Mellein can be produced in vitro via the enzymatic reaction of recombinant SACE5532, and can also be generated in the culture system of E. coli cells co-expressing SACE5532 and Sfp[1].
Mellein is only produced in trace amounts by Aspergillus ochraceus NRRL 3174 on yellow corn, whereas it is not detected in wheat, peanuts or soybeans[2].
Mellein exhibits broad-spectrum in vitro antimicrobial activity, with MIC values of 1.9-15.6 μg/mL against human bacterial pathogens, 7.8-15.6 μg/mL against human fungal pathogens, and 1.9-31.25 μg/mL against phytopathogenic Xanthomonas pathovars[3].
Mellein disrupts the cell wall and cellular integrity of Xanthomonas oryzae pv. oryzicola strain Y2[3].
Mellein binds to multiple Xanthomonas target proteins with binding energies ranging from -3.188 to -4.520 kcal/mol, indicating that it may inhibit bacterial protein synthesis, enhance host immunity, inactivate microbial enzymes, and suppress bacterial motility, virulence, and biofilm formation[3].
Mellein (1-100 μg/disk; 12 days) inhibits mycelial growth of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 480-33-1
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Appearance Solid
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Peso molecular 178.18
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Fòrmula C10H10O3
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Color White to off-white
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SMILES
O=C1C2=C(O)C=CC=C2C[C@@H](C)O1
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Synonyms
(R)-Mellein
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Structure Classification
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Initial Source
Aspergillus melleus
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Molecules
Identification of Active Anti-Inflammatory Compounds in Sweet Potato Storage Roots Extracted with Ethanol via LC-Q-TOF-MS. [Abstract]2026 Jan 28;31(3):456. PMID: 41683433
Solvente y solubilidad
DMSO : 100 mg/mL (561.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (14.03 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (290 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Sun H, et al. Synthesis of (R)-mellein by a partially reducing iterative polyketide synthase. Journal of the American Chemical Society. 2012 Jul 25;134(29):11924-7. [Content Brief]
[3]. Saraswathi M, et al. Isolation, purification and structural elucidation of Mellein from endophytic fungus Lasiodiplodia theobromae strain (SJF-1) and its broad-spectrum antimicrobial and pharmacological properties. Letters in applied microbiology. 2022 Dec;75(6):1475-1485. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6123 mL | 28.0615 mL | 56.1230 mL | 140.3076 mL |
| 5 mM | 1.1225 mL | 5.6123 mL | 11.2246 mL | 28.0615 mL | |
| 10 mM | 0.5612 mL | 2.8062 mL | 5.6123 mL | 14.0308 mL | |
| 15 mM | 0.3742 mL | 1.8708 mL | 3.7415 mL | 9.3538 mL | |
| 20 mM | 0.2806 mL | 1.4031 mL | 2.8062 mL | 7.0154 mL | |
| 25 mM | 0.2245 mL | 1.1225 mL | 2.2449 mL | 5.6123 mL | |
| 30 mM | 0.1871 mL | 0.9354 mL | 1.8708 mL | 4.6769 mL | |
| 40 mM | 0.1403 mL | 0.7015 mL | 1.4031 mL | 3.5077 mL | |
| 50 mM | 0.1122 mL | 0.5612 mL | 1.1225 mL | 2.8062 mL | |
| 60 mM | 0.0935 mL | 0.4677 mL | 0.9354 mL | 2.3385 mL | |
| 80 mM | 0.0702 mL | 0.3508 mL | 0.7015 mL | 1.7538 mL | |
| 100 mM | 0.0561 mL | 0.2806 mL | 0.5612 mL | 1.4031 mL |
Mellein Related Classifications
HY-N3300 Related Classifications
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