ML339
Based on 5 publication(s) in Google Scholar
ML339 is a selective CXCR6 antagonist with an IC50 of 140 nM. ML339 antagonizes β-arrestin recruitment and cAMP signaling pathway of human CXCR6 receptor induced by CXCL16, with IC50 of 0.3 μM and 1.4 μM, respectively. ML339 shows weaker activity against the recruitment of β-arrestin in mouse CXCR6 receptors, with an IC50 of 18 μM. ML339 has no inhibitory effect on CXCR5，CXCR4，CXCR6 and apelin receptor (APJ), with IC50 >79 μM. ML339 has the potential to promote the development of prostate cancer research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.88%
- No. CAS: 2579689-83-9
- Fòrmula: C26H32ClN3O5
- Peso molecular:502.00
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
HY-15319
AMG 487CXCR3 Antagonist -
HY-10046
PlerixaforCXCR4 Antagonist -
HY-50101A
Mavorixafor trihydrochlorideCXCR4 Antagonist -
HY-50912
Plerixafor octahydrochlorideCXCR4 Antagonist -
HY-101458A
IT1t dihydrochlorideCXCR4 Antagonist -
HY-100558
Bafilomycin A1V-ATPase Inhibitor -
HY-116835
BI-6901CCR10 Antagonist -
HY-13848
RugocrixanCX3CR1 Antagonist -
HY-P72961
CXCL16 Protein, Human (HEK293, His) -
HY-16931
SMIFH2Formin Inhibitor -
HY-P7032
IL-12 Protein, Human (HEK293) -
HY-123918
JMS-17-2CX3CR1 Antagonist
Publications Citing Use of MedChemExpress (MCE) ML339
More
Customer Validation & Images
-
In Vivo Efficacy Study
Ver todos los productos específicos de isoformas Arrestin
More
Actividad biológica
Descripciòn
IC50 & Target
[1]|
CXCR6 140 nM (IC50) |
In Vitro
ML339 doesn’t have a broad affinity for G protein-coupled receptors (GPCRs) and only shows moderate activity for 5-HT2B and DAT at a concentration of 10 μM in competitive binding assays[1].
ML339 exhibits high plasma protein binding[1].
ML339 shows good stability in human plasma but only moderate stability in mouse plasma[1].
ML339 is non-toxic to immortalized Fa2-N4 human liver cells, with an LC50 greater than 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
No. CAS 2579689-83-9
-
Appearance Solid
-
Peso molecular 502.00
-
Fòrmula C26H32ClN3O5
-
Color White to off-white
-
SMILES
O=C(C1=CC(OC)=C(C(OC)=C1)OC)N[C@@H]2C[C@@H](CCC[C@H]3C2)N3CC(NC4=CC=CC=C4Cl)=O
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
-
Journal Impact Factor
-
Most Recent
-
Immunity
Spatiotemporal dynamics of CXCL10 encode contextual immune information revealed by the genetically encoded fluorescent sensor. [Abstract]2025 Sep 9;58(9):2320-2335.e9. PMID: 40818452 -
Adv Sci (Weinh)
Retinoic Acid Reprograms Mast Cells Toward a Proinflammatory State to Enhance Antitumor Immunity. [Abstract]2025 Nov 27:e09340. PMID: 41309490 -
J Immunother Cancer
2024 Dec 4;12(12):e009629. PMID: 39631847
ML339 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Dec 4;12(12):e009629. [Abstract]
The representative density plot of CD45.1-TH9 cells in the lungs 48 hours after intravenous injection of CD45.1-TH9 cells into healthy CD45.2 mice. Healthy CD45.2 mice were received intraperitoneal (i.p.) injection of 1 mg/kg ML339, C-021, R243 and AMD3100, 24 hours before T cell transfer.
-
-
Solvente y solubilidad
In Vitro:
DMSO : 75 mg/mL (149.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureza y Documentación
-
Ficha de datos (275 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. Paul M Hershberger, et al. Probing the CXCR6/CXCL16 Axis: Targeting Prevention of Prostate Cancer Metastasis. [Content Brief]
[2]. Peddibhotla S, et al. Discovery of small molecule antagonists of chemokine receptor CXCR6 that arrest tumor growth in SK-HEP-1 mouse xenografts as a model of hepatocellular carcinoma. Bioorg Med Chem Lett. 2020 Feb 15;30(4):126899. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9920 mL | 9.9602 mL | 19.9203 mL | 49.8008 mL |
| 5 mM | 0.3984 mL | 1.9920 mL | 3.9841 mL | 9.9602 mL | |
| 10 mM | 0.1992 mL | 0.9960 mL | 1.9920 mL | 4.9801 mL | |
| 15 mM | 0.1328 mL | 0.6640 mL | 1.3280 mL | 3.3201 mL | |
| 20 mM | 0.0996 mL | 0.4980 mL | 0.9960 mL | 2.4900 mL | |
| 25 mM | 0.0797 mL | 0.3984 mL | 0.7968 mL | 1.9920 mL | |
| 30 mM | 0.0664 mL | 0.3320 mL | 0.6640 mL | 1.6600 mL | |
| 40 mM | 0.0498 mL | 0.2490 mL | 0.4980 mL | 1.2450 mL | |
| 50 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9960 mL | |
| 60 mM | 0.0332 mL | 0.1660 mL | 0.3320 mL | 0.8300 mL | |
| 80 mM | 0.0249 mL | 0.1245 mL | 0.2490 mL | 0.6225 mL | |
| 100 mM | 0.0199 mL | 0.0996 mL | 0.1992 mL | 0.4980 mL |
ML339 Related Classifications
HY-122197 Related Classifications
Powered by Bioz