UNC10142
Based on 1 publication(s) in Google Scholar
UNC10142 is a selective CHD1 antagonist with an IC50 of 1.7 μM and a Kd of 4.3 μM. UNC10142 exhibits anticancer activity against PTEN-deficient prostate cancer but shows no effect on PTEN-intact prostate cancer. UNC10142 can be used in studies related to PTEN-deficient prostate cancer.
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- Pureza: 99.74%
- No. CAS: 3118919-69-7
- Fòrmula: C33H52N6O3
- Peso molecular:580.80
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) UNC10142
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Actividad biológica
Descripciòn
In Vitro
UNC10142 (1 h) inhibits the interaction between recombinant CHD1 tandem chromatin domains and H3K4me2 peptide in TR-FRET assays, with an IC50 of 1.7 μM[1].
UNC10142 (750-1000 μM) binds directly to the tandem chromatin domains of recombinant CHD1, with a measured Kd value of 4.3 μM determined by ITC[1].
UNC10142 exhibits much higher selectivity for CHD1 than most of the tested Kme reader domains, with only 6-fold weaker binding affinity for the PHF19 Tudor domain[1].
UNC10142 (75 μM; 24 h) selectively reduces the viability of PTEN-deficient PC-3 and LNCaP prostate cancer cells, while exerting minimal effects on PTEN-intact DU145 prostate cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PTEN-deficient (PC-3, LNCaP) and PTEN-intact (DU145) prostate cancer cell lines
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Concentration:Dose-response series; 75 μM
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Incubation Time:24 h
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Result:Reduced viability of PTEN-deficient PC-3 cells by 87% and PTEN-deficient LNCaP cells by 54% at 75 μM, while sparing PTEN-intact DU145 cells at the same concentration.
Chemical Information
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No. CAS 3118919-69-7
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Appearance Solid
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Peso molecular 580.80
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Fòrmula C33H52N6O3
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Color White to off-white
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SMILES
COC1=C(OCCCN2CCCCC2)C=C(N=C(N3CCCCCCCC3)N=C4N(C)C5CCN(C(C)=O)CC5)C4=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Biochem Pharmacol
CHD1 regulates the inflammatory response in macrophages and functions as a pharmacological target during sepsis. [Abstract]2026 Aug;250(Pt 1):118006. PMID: 42034314
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (172.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (285 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7218 mL | 8.6088 mL | 17.2176 mL | 43.0441 mL |
| 5 mM | 0.3444 mL | 1.7218 mL | 3.4435 mL | 8.6088 mL | |
| 10 mM | 0.1722 mL | 0.8609 mL | 1.7218 mL | 4.3044 mL | |
| 15 mM | 0.1148 mL | 0.5739 mL | 1.1478 mL | 2.8696 mL | |
| 20 mM | 0.0861 mL | 0.4304 mL | 0.8609 mL | 2.1522 mL | |
| 25 mM | 0.0689 mL | 0.3444 mL | 0.6887 mL | 1.7218 mL | |
| 30 mM | 0.0574 mL | 0.2870 mL | 0.5739 mL | 1.4348 mL | |
| 40 mM | 0.0430 mL | 0.2152 mL | 0.4304 mL | 1.0761 mL | |
| 50 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8609 mL | |
| 60 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7174 mL | |
| 80 mM | 0.0215 mL | 0.1076 mL | 0.2152 mL | 0.5381 mL | |
| 100 mM | 0.0172 mL | 0.0861 mL | 0.1722 mL | 0.4304 mL |
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