2. Cell Cycle/DNA Damage
    Stem Cell/Wnt
    PI3K/Akt/mTOR
    Apoptosis
  3. Casein Kinase
    Akt
    Wnt
    Apoptosis
  4. Hematein

Hematein 

Cat. No.: HY-119751 Purity: 74.90%
COA Handling Instructions

Hematein is a oxidation product of hematoxylin acted as a dye. Hematein is an allosteric casein kinase II inhibitor with an IC50 of 0.74 μM. Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway and increases apoptosis in lung cancer cells.

For research use only. We do not sell to patients.

Hematein Chemical Structure

Hematein Chemical Structure

CAS No. : 475-25-2

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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

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Description

Hematein is a oxidation product of hematoxylin acted as a dye[1]. Hematein is an allosteric casein kinase II inhibitor with an IC50 of 0.74 μM. Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway and increases apoptosis in lung cancer cells[2].

IC50 & Target[2]

CKII

0.74 μM (IC50)

In Vitro

Hematein (10-100 μM; 14 days) inhibits cells growth in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) induces apoptosis in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 10-100 μM
Incubation Time: 14 days
Result: Inhibited cells growth.

Apoptosis Analysis[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 50 and 100 μM
Incubation Time: 48 hours
Result: Induced apoptosis in A427 lung cancer cells.

Western Blot Analysis[1]

Cell Line: A427 (HTB-53) cell line
Concentration: 50 and 100 μM
Incubation Time: 48 hours
Result: Inhibited CK2-specific Akt phosphorylation.
In Vivo

Hematein (intraperitoneal injections; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth in A427 lung cancer cell xenografts[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic BALB/c nude mice (6-week-old) [1]
Dosage: 50 mg/kg
Administration: Intraperitoneal injections; twice a week for 6 weeks
Result: Inhibited tumor growth in A427 lung cancer cell xenografts.
Molecular Weight

300.26

Appearance

Solid

Formula

C16H12O6
CAS No.
SMILES

O=C1C=C2CC3(C(C4=CC=C(C(O)=C4OC3)O)=C2C=C1O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (333.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3304 mL 16.6522 mL 33.3045 mL
5 mM 0.6661 mL 3.3304 mL 6.6609 mL
10 mM 0.3330 mL 1.6652 mL 3.3304 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Hematein475-25-2Casein KinaseAktWntApoptosisPKBProtein kinase BInhibitorinhibitorinhibit

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