CD28-IN-3
CD28-IN-3 is a CD28 inhibitor with an IC50 of 7.80 μM and a Kd of 52.45 μM. CD28-IN-3 selectively blocks the CD28-B7 interaction, thereby inhibiting CD28-mediated costimulation. CD28-IN-3 suppresses the production of proinflammatory cytokines IFN-γ, IL-2 and TNF-α. CD28-IN-3 can be used in the research of checkpoint-resistant cancers.
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- CAS 番号: 850550-70-8
- 分子式: C17H24N4OS2
- 分子量:364.53
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
CD28-IN-3 (Compound 22VS) (0-100 μM; 2 h) inhibits the interactions of membrane-bound CD28-CD80 and CD28-CD86 in CHO-K1 cells, with corresponding submicromolar IC50 values of 1.48 μM and 1.39 μM, respectively[1].
CD28-IN-3 (10-300 μM; 24 h) shows no cytotoxicity against human Jurkat T lymphocytes even at concentrations up to 300 μM following a 24 h incubation[1].
CD28-IN-3 (0-200 μM; 5 h) inhibits CD28-mediated T cell costimulation in the Jurkat-Raji co-culture system, with an IC50 of 6.96 μM[1].
CD28-IN-3 (10-50 μM; 48 h) inhibits the release of CD28-dependent proinflammatory cytokines (IFN-γ, IL-2, TNF-α) in a dose-dependent manner in 3D A549 tumor-PBMC co-culture systems and human PBMC-mucosa co-culture systems[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Human Jurkat T lymphocytes
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Concentration:10, 30, 100 and 300 μM
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Incubation Time:24 h (37°C)
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Result:Maintained cell viability above 85% at all tested concentrations, showing no measurable cytotoxicity.
化学情報
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CAS 番号 850550-70-8
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分子量 364.53
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分子式 C17H24N4OS2
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SMILES
CCC(NC(CSC1=NN=C(S1)NC2=CC=C(C=C2)C(C)C)=O)C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
CD28-IN-3 関連分類
HY-181507 関連分類
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