エンベリン
Based on 7 publication(s) in Google Scholar
Embelin (Embelic acid), a potent, nonpeptidic XIAP inhibitor (IC50=4.1 μM), inhibits cell growth, induces apoptosis, and activates caspase-9 in prostate cancer cells with high levels of XIAP. Embelin blocks NF-kappaB signaling pathway leading to suppression of NF-kappaB-regulated antiapoptotic and metastatic gene products. Embelin also induces autophagic and apoptotic cell death in human oral squamous cell carcinoma cells.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.01%
- CAS 番号: 550-24-3
- 分子式: C17H26O4
- 分子量:294.39
-
保管条件:
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
HY-17394
CisplatinDNA Alkylator -
HY-15989
SM-164IAP Antagonist -
HY-12600
AZD5582 -
HY-10219
RapamycinmTOR Inhibitor -
HY-17393
CarboplatinDNA Synthesis Inhibitor -
HY-N0414
TrigonellineNrf2 Inhibitor -
HY-N1497
PlumbaginAnticancer Agent -
HY-13629
EtoposideTopoisomerase II Inhibitor -
HY-10358
MK-2206 dihydrochlorideAllosteric Akt Inhibitor -
HY-135303
GLPG1205GPR84 Antagonist -
HY-15893
DMOGHIF-PH Inhibitor -
HY-K1089
Cell Senescence β-Galactosidase Staining Kit
MedChemExpress（MCE）の使用を引用している文献 Embelin
More- Cell Res. 2021 Mar;31(3):291-311. [Abstract]
- Autophagy. 2025 Oct 25. [Abstract]
- Sci Adv. 2025 May 2;11(18):eadu6676. [Abstract]
- Pharmacol Res. 2020 May:155:104751. [Abstract]
- Phytomedicine. 2026 May 14:157:158282. [Abstract]
- Mol Neurobiol. 2014 Apr;49(2):1087-101. [Abstract]
- Mol Pharm. 2023 Sep 4;20(9):4574-4586. [Abstract]
Customer Validation & Images
-
WB
生物活性
製品説明
IC50 & Target
[1]|
XIAP 4.1 μM (IC50) |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
1.79 μM
Compound: 5
|
Cytotoxicity against human A2780 cells after 96 hrs by MTT assay
Cytotoxicity against human A2780 cells after 96 hrs by MTT assay
|
[PMID: 17125236] |
| A549 | IC50 |
0.21 μM
Compound: 2
|
Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method
Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method
|
[PMID: 25765759] |
| A549 | IC50 |
1.47 μM
Compound: 5
|
Cytotoxicity against human A549 cells after 96 hrs by MTT assay
Cytotoxicity against human A549 cells after 96 hrs by MTT assay
|
[PMID: 17125236] |
| Bel-7402 | IC50 |
1.66 μM
Compound: 5
|
Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay
Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay
|
[PMID: 17125236] |
| BGC-823 | IC50 |
1.69 μM
Compound: 5
|
Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay
Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay
|
[PMID: 17125236] |
| CHO | EC50 |
0.421 μM
Compound: 2
|
Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
|
[PMID: 31721581] |
| CHO | EC50 |
5 μM
Compound: 2
|
Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as beta-arrestin 2 recruitment by beta-galactosidase based PathHunter assay
Agonist activity at recombinant human GPR84 expressed in CHO cells assessed as beta-arrestin 2 recruitment by beta-galactosidase based PathHunter assay
|
[PMID: 31721581] |
| HCT-8 | IC50 |
1.49 μM
Compound: 5
|
Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay
Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay
|
[PMID: 17125236] |
| HEK293 | EC50 |
0.089 μM
Compound: 2
|
Agonist activity at recombinant human HA-tagged GPR84 stably expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 60 mins by HTRF assay
Agonist activity at recombinant human HA-tagged GPR84 stably expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation measured after 60 mins by HTRF assay
|
[PMID: 31721581] |
| HEK293 | EC50 |
0.63 μM
Compound: 2
|
Agonist activity at recombinant human GPR84 expressed in HEK293 cells co-expressing Gqi5 assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
Agonist activity at recombinant human GPR84 expressed in HEK293 cells co-expressing Gqi5 assessed as inhibition of forskolin-induced cAMP accumulation by fluo-4AM dye based FLIPR assay
|
[PMID: 31721581] |
| Jurkat | IC50 |
20 μM
Compound: Embelin
|
Inhibitory concentration of compound against Jurkat-Vec cells
Inhibitory concentration of compound against Jurkat-Vec cells
|
[PMID: 15115387] |
| LNCaP | IC50 |
5.7 μM
Compound: Embelin
|
Compound was tested for inhibition of LnCap prostate cancer cells
Compound was tested for inhibition of LnCap prostate cancer cells
|
[PMID: 15115387] |
| LNCaP | IC50 |
5.7 μM
Compound: Embelin
|
Inhibitory concentration of compound against growth of LNCaP cells in vitro
Inhibitory concentration of compound against growth of LNCaP cells in vitro
|
[PMID: 15115387] |
| PC-3 | IC50 |
3.7 μM
Compound: Embelin
|
Compound was tested for inhibition of PC-3 prostate cancer cells
Compound was tested for inhibition of PC-3 prostate cancer cells
|
[PMID: 15115387] |
| PC-3 | IC50 |
3.7 μM
Compound: Embelin
|
Inhibitory concentration of compound against growth of PC-3 cells in vitro
Inhibitory concentration of compound against growth of PC-3 cells in vitro
|
[PMID: 15115387] |
| PrEC | IC50 |
20.1 μM
Compound: Embelin
|
Inhibitory concentration of compound against growth of PrEC cells in vitro
Inhibitory concentration of compound against growth of PrEC cells in vitro
|
[PMID: 15115387] |
| PrEC | IC50 |
20.1 μM
Compound: Embelin
|
Selectivity in normal human prostate epithelial cells (PrEC)
Selectivity in normal human prostate epithelial cells (PrEC)
|
[PMID: 15115387] |
| WI-38 | IC50 |
19.3 μM
Compound: Embelin
|
Inhibitory concentration of compound against growth of WI-38 cells in vitro
Inhibitory concentration of compound against growth of WI-38 cells in vitro
|
[PMID: 15115387] |
| WI-38 | IC50 |
19.3 μM
Compound: Embelin
|
Selectivity in normal human fibroblast cell line, WI-38 cells
Selectivity in normal human fibroblast cell line, WI-38 cells
|
[PMID: 15115387] |
体外実験
化学情報
-
CAS 番号 550-24-3
-
性状 Solid
-
分子量 294.39
-
分子式 C17H26O4
-
Color Yellow to orange
-
SMILES
O=C1C(O)=C(CCCCCCCCCCC)C(C(O)=C1)=O
-
別名
Embelin; Embelic acid; Emberine; NSC 91874
-
Structure Classification
-
Initial Source
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
Publications (7)
-
Journal Impact Factor
-
Most Recent
-
Cell Res
2021 Mar;31(3):291-311. PMID: 33299139 -
Autophagy
XIAP-ULK1-Mediated mitophagy modulates carnitine metabolism to mitigate diabetic kidney disease. [Abstract]2025 Oct 25. PMID: 41139215 -
Sci Adv
In situ protein corona-camouflaged supramolecular assemblies remodel thrombotic microenvironment for improved arterial homeostasis. [Abstract]2025 May 2;11(18):eadu6676. PMID: 40315315 -
Pharmacol Res
Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]2020 May:155:104751. PMID: 32151678 -
Phytomedicine
5-O-Methylembelin disrupts the ACO1-TPI1 interaction to promote ferroptosis in clear cell renal cell carcinoma. [Abstract]2026 May 14:157:158282. PMID: 42166976 -
Mol Neurobiol
Embelin suppresses dendritic cell functions and limits autoimmune encephalomyelitis through the TGF-β/β-catenin and STAT3 signaling pathways. [Abstract]2014 Apr;49(2):1087-101. PMID: 24258405
Embelin purchased from MedChemExpress. Usage Cited in: Mol Neurobiol. 2014 Apr;49(2):1087-101. [Abstract]
Embelin (EB) promotes TGF-β/β-catenin and inhibits STAT3 signaling in mDCs. DCs are treated with 10, 30, and 60 μM EB to investigate its effect on p-STAT3, TGF-β, β-catenin, and p-GSK-3β signaling (diluted 1:1,000).
-
Mol Pharm
Construction of SLC16A1/3 Targeted Gallic Acid-Iron-Embelin Nanoparticles for Regulating Glycolysis and Redox Pathways in Cervical Cancer. [Abstract]2023 Sep 4;20(9):4574-4586. PMID: 37307591
溶剤 & 溶解度
体外:
DMSO : ≥ 50 mg/mL (169.84 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.49 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (270 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Nikolovska-Coleska Z, et al. Discovery of embelin as a cell-permeable, small-molecular weight inhibitor of XIAP through structure-based computational screening of a traditional herbal medicine three-dimensional structure database. J Med Chem. 2004;47(10):2430-2440. [Content Brief]
[2]. Ahn KS, et al. Embelin, an inhibitor of X chromosome-linked inhibitor-of-apoptosis protein, blocks nuclear factor-kappaB (NF-kappaB) signaling pathway leading to suppression of NF-kappaB-regulated antiapoptotic and metastatic gene products. Mol Pharmacol. 2007;71(1):209-219. [Content Brief]
[3]. Lee YJ, et al. XIAP inhibitor embelin induces autophagic and apoptotic cell death in human oral squamous cell carcinoma cells. Environ Toxicol. 2017;32(11):2371-2378. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3969 mL | 16.9843 mL | 33.9685 mL | 84.9214 mL |
| 5 mM | 0.6794 mL | 3.3969 mL | 6.7937 mL | 16.9843 mL | |
| 10 mM | 0.3397 mL | 1.6984 mL | 3.3969 mL | 8.4921 mL | |
| 15 mM | 0.2265 mL | 1.1323 mL | 2.2646 mL | 5.6614 mL | |
| 20 mM | 0.1698 mL | 0.8492 mL | 1.6984 mL | 4.2461 mL | |
| 25 mM | 0.1359 mL | 0.6794 mL | 1.3587 mL | 3.3969 mL | |
| 30 mM | 0.1132 mL | 0.5661 mL | 1.1323 mL | 2.8307 mL | |
| 40 mM | 0.0849 mL | 0.4246 mL | 0.8492 mL | 2.1230 mL | |
| 50 mM | 0.0679 mL | 0.3397 mL | 0.6794 mL | 1.6984 mL | |
| 60 mM | 0.0566 mL | 0.2831 mL | 0.5661 mL | 1.4154 mL | |
| 80 mM | 0.0425 mL | 0.2123 mL | 0.4246 mL | 1.0615 mL | |
| 100 mM | 0.0340 mL | 0.1698 mL | 0.3397 mL | 0.8492 mL |
Embelin 関連分類
HY-17473 関連分類
Powered by Bioz