EPI-X4
Based on 1 publication(s) in Google Scholar
EPI-X4 (hSA408–423 peptide) is an antagonist for C-X-C motif chemokine receptor 4 (CXCR4) with IC50 of 8.6 μM. EPI-X4 blocks the CXCL12-mediated signaling, inhibits chemokine-mediated migration and invasion of leukemia cell. EPI-X4 exhibits anti-inflammatory activity in mouse model. EPI-X4 exhibits antiviral activity against CXCR4-tropic HIV with IC50 of 8.6 μM.
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- 純度: 98.91%
- CAS 番号: 930280-31-2
- 分子式: C84H144N22O23
- 分子量:1830.18
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 EPI-X4
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生物活性
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CXCR4 8.6 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
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| TZM | IC50 |
>10000 nM
Compound: EPI-X4; ALB408-423
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Antiviral activity against HIV-1 NL4-3 infected in human TZM-bl cells assessed as inhibition of viral replication preincubated for 15 mins followed by viral infection and measured after 4320 mins by beta-gal screening assay
Antiviral activity against HIV-1 NL4-3 infected in human TZM-bl cells assessed as inhibition of viral replication preincubated for 15 mins followed by viral infection and measured after 4320 mins by beta-gal screening assay
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[PMID: 37940118] |
EPI-X4 (200 μM) blocks the CXCR4 12G5 epitope on BCWM mutant cells and impairs migration[2].
EPI-X4 (0-100 μM) inhbits the ERK and AKT signaling in WM cells[2].
EPI-X4 (200 μM, 24 h) induces a shift towards aerobic glycolysis and downregulates pathways that are enriched for genes involved in Toll receptor, NK-κB, MAPK, AMPK, mTOR, and CXCR4/chemokine mediated signaling in BCWM.1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 930280-31-2
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性状 Solid
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分子量 1830.18
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分子式 C84H144N22O23
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Color White to off-white
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別名
hSA(408–423) peptide
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配列
Leu-Val-Arg-Tyr-Thr-Lys-Lys-Val-Pro-Gln-Val-Ser-Thr-Pro-Thr-Leu
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シーケンスの短縮
LVRYTKKVPQVSTPTL
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Wound Repair Regen
TWEAK regulates the functions of hair follicle stem cells via the Fn14-Wnt/β-catenin-CXCR4 signalling axis. [Abstract]2025 May-Jun;33(3):e70032. PMID: 40325995
溶剤 & 溶解度
DMSO : 100 mg/mL (54.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (277 KB)
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SDS (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Zirafi O, et al., Discovery and characterization of an endogenous CXCR4 antagonist. Cell Rep. 2015 May 5;11(5):737-47. [Content Brief]
[2]. Kaiser, L. M., et al., (2021). Targeting of CXCR4 by the Naturally Occurring CXCR4 Antagonist EPI-X4 in Waldenström's Macroglobulinemia. Cancers, 13(4), 826. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5464 mL | 2.7320 mL | 5.4639 mL | 13.6599 mL |
| 5 mM | 0.1093 mL | 0.5464 mL | 1.0928 mL | 2.7320 mL | |
| 10 mM | 0.0546 mL | 0.2732 mL | 0.5464 mL | 1.3660 mL | |
| 15 mM | 0.0364 mL | 0.1821 mL | 0.3643 mL | 0.9107 mL | |
| 20 mM | 0.0273 mL | 0.1366 mL | 0.2732 mL | 0.6830 mL | |
| 25 mM | 0.0219 mL | 0.1093 mL | 0.2186 mL | 0.5464 mL | |
| 30 mM | 0.0182 mL | 0.0911 mL | 0.1821 mL | 0.4553 mL | |
| 40 mM | 0.0137 mL | 0.0683 mL | 0.1366 mL | 0.3415 mL | |
| 50 mM | 0.0109 mL | 0.0546 mL | 0.1093 mL | 0.2732 mL |
EPI-X4 関連分類
HY-P10415 関連分類
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