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製品番号: HY-160005

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LMP517

(別名: NSC 781517)

カスタマーレビュー

Based on 1 Customer Validation

LMP517 (NSC 781517) is a potent and non-camptothecin Topoisomerase I and II (TOP1 and TOP2) dual inhibitor. LMP517 can induce TOP1 and TOP2 cleavage complexes (TOP1ccs and TOP2ccs). LMP517 can induce cancer cells DNA damage and γH2AX production. LMP517 can be used for the research of cancer, such as small cell lung cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.58%
CAS 番号: 1883730-99-1
分子式: C₂₂H₁₉FN₂O₅
分子量:410.40

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

LMP517 構造式

CAS 番号: 1883730-99-1

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
化学情報
溶剤 & 溶解度
- 体外
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target^[1]

Topoisomerase I

Topoisomerase II

Topoisomerase アイソフォーム固有の製品をすべて表示 :

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	GI₅₀	0.033 μM Compound: 29	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 26906474]
HCT-116	GI₅₀	0.019 μM Compound: 29	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 26906474]
HOP-62	GI₅₀	0.036 μM Compound: 29	Cytotoxicity against human HOP62 cells by MTT assay Cytotoxicity against human HOP62 cells by MTT assay	[PMID: 26906474]
MCF7	GI₅₀	<0.01 μM Compound: 29	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 26906474]
OVCAR-3	GI₅₀	0.076 μM Compound: 29	Cytotoxicity against human OVCAR3 cells by MTT assay Cytotoxicity against human OVCAR3 cells by MTT assay	[PMID: 26906474]
SF-539	GI₅₀	0.041 μM Compound: 29	Cytotoxicity against human SF539 cells by MTT assay Cytotoxicity against human SF539 cells by MTT assay	[PMID: 26906474]
SN12C	GI₅₀	0.107 μM Compound: 29	Cytotoxicity against human SN12C cells by MTT assay Cytotoxicity against human SN12C cells by MTT assay	[PMID: 26906474]
UACC-62	GI₅₀	0.028 μM Compound: 29	Cytotoxicity against human UACC62 cells by MTT assay Cytotoxicity against human UACC62 cells by MTT assay	[PMID: 26906474]

体外実験

LMP517 (0-125 nM, 72 h) shows IC₅₀ values of 11, 18 and 32 nM in DT40 tdp2, tdp1 and WT cells^[1].
LMP517 (0.1-30 μM, 1 h) induces TOP1cc, TOP2cc, TOP2α and TOP2βccs production in HCT116 and TK6 cells^[1].
LMP517 (0.05-1 μM, 1 h) induces DNA damage and histone γH2AX production in HCT116 FUCCI cells^[1].
LMP517 (1 μM, 1 h) induces TOP1cc and DPC production in CCR-CEM cells^[2].
LMP517 (0.1-1 μM, 0.5-1 h) induces γH2AX production in HCT1116 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[2]

Cell Line:

HCT1116 cells
Concentration:

1 μM
Incubation Time:

1 h
Result:

IncreasedγH2AX production.

体内実験

LMP517 (10 mg/kg, i.v., daily for 5 days) inhibits tumor growth and prolongs survival in H82 xenograft tumor mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

H82 xenograft tumor mice models^[1]
Dosage:

10 mg/kg
Administration:

Intravenously injection, daily for 5 days
Result:

Induced a reduction in tumor growth.
Prolonged survival of mice.
Had marginal body weight lost.

化学情報

CAS 番号 1883730-99-1
性状 Solid
分子量 410.40
分子式 C₂₂H₁₉FN₂O₅
Color Deep purple to brown
SMILES

O=C1C2=CC3=C(C=C2C4=C1C5=C(C(N4CCCNCCO)=O)C=C(F)C=C5)OCO3
別名

NSC 781517
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 44 mg/mL (107.21 mM; ultrasonic and adjust pH to 1 with TFA; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4366 mL

12.1832 mL

24.3665 mL

5 mM

0.4873 mL

2.4366 mL

4.8733 mL

10 mM

0.2437 mL

1.2183 mL

2.4366 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Marzi L, et al. The Indenoisoquinoline LMP517: A Novel Antitumor Agent Targeting both TOP1 and TOP2. Mol Cancer Ther. 2020 Aug;19(8):1589-1597. [Content Brief]

[2]. Marzi L, et al. Novel Fluoroindenoisoquinoline Non-Camptothecin Topoisomerase I Inhibitors. Mol Cancer Ther. 2018 Aug;17(8):1694-1704. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4366 mL	12.1832 mL	24.3665 mL	60.9162 mL
	5 mM	0.4873 mL	2.4366 mL	4.8733 mL	12.1832 mL
	10 mM	0.2437 mL	1.2183 mL	2.4366 mL	6.0916 mL
	15 mM	0.1624 mL	0.8122 mL	1.6244 mL	4.0611 mL
	20 mM	0.1218 mL	0.6092 mL	1.2183 mL	3.0458 mL
	25 mM	0.0975 mL	0.4873 mL	0.9747 mL	2.4366 mL
	30 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0305 mL
	40 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.5229 mL
	50 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2183 mL
	60 mM	0.0406 mL	0.2031 mL	0.4061 mL	1.0153 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7615 mL
	100 mM	0.0244 mL	0.1218 mL	0.2437 mL	0.6092 mL

Keywords

MOQ

Minimum order quantity

100 mg

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Other size

お問い合わせ

Amount: USD 0.00

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
溶剤 & 溶解度
- 体外実験
純度とドキュメンテーション
参考文献

製品名:
製品番号:
数量:
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LMP517

Topoisomerase アイソフォーム固有の製品をすべて表示

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示

生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

LMP517 関連分類

HY-160005 関連分類