JQ1-FITC TFA
JQ1-FITC TFA is a BRD4-binding fluorescent tracer. JQ1-FITC TFA binds to BRD4 BD1, BD2, recombinant bromodomains, and endogenous BRD4 in cell lysates. JQ1-FITC TFA can be used for the research of breast cancer and cancer (Ex/Em = 495/525 nm).
For research use only. We do not sell to patients.
- Formula: C52H51ClF3N7O11S2
- Molecular Weight:1106.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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BRD4 BD1 |
BRD4 BD2 |
JQ1-FITC (0-100 nM; 2 h) TFA binds specifically to recombinant human BRD4(BD1) and BRD4(BD2) bromodomains with measurable apparent equilibrium dissociation constants[1].
JQQ1-FITIC (0-2200 nM; 2 h) TFA binds specifically to endogenous BRD4[1].
JQ1-FITC (20 nM; 2 h) TFA functions as a fluorescent tracer to enable TR-FRET ligand displacement assays for measuring test compound binding affinity to recombinant human BRD4(BD1) and BRD4(BD2) bromodomains[1].
JQ1-FITC (700 nM; 1 h) TFA enables quantitative TR-FRET-based measurement of endogenous BRD4 levels in MCF7 and MDA-MB-231 cell lysates[1].
JQ1-FITC (1750 nM; 1 h) TFA enables accurate quantitative TR-FRET-based measurement of endogenous BRD4 levels in MDA-MB-231 cell lysates without the need for compound washout, over incubation times ranging from 30 min to 10 h[1].
JQ1-FITC (Serial dilutions up to 100 nM; 2 h) TFA binds to recombinant GST-BRD4(BD1) and GST-BRD4 (BD2) protein, with the equilibrium dissociation constant adjusted for the dimeric GST structure[2].
JQ1-FITC (20 nM; 2 h) TFA acts as a tracer for endogenous BRD4 binding in HEK293T cell lysate, enabling quantitative profiling of inhibitor domain selectivity and affinity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 1106.58
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Formula C52H51ClF3N7O11S2
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SMILES
O=C(C[C@@H]1N=C(C2=C(N3C1=NN=C3C)SC(C)=C2C)C4=CC=C(C=C4)Cl)NCCCOCCOCCOCCCNC(NC5=CC=C(C6=C(C=C7)C(OC8=C6C=CC(O)=C8)=CC7=O)C(C(O)=O)=C5)=S.OC(C(F)(F)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
JQ1-FITC TFA Related Classifications
HY-D3393 Related Classifications
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