Mecillinam hydrochloride
Based on 4 publication(s) in Google Scholar
Mecillinam hydrochloride is a β-lactam Antibiotic. Mecillinam hydrochloride inhibits PBP2. Mecillinam hydrochloride exhibits activity against Gram-negative Enterobacteriaceae, and NDM-/OXA-producing E. coli and K. pneumoniae, and can eradicate catheter/bladder mucosa biofilms and clear planktonic bacteria in urine. Mecillinam hydrochloride acts synergistically with β-lactams and Avibactam (HY-14879), enhancing the activity of β-lactams against E. coli in vitro/in vivo. Mecillinam hydrochloride can be used in research on catheter-associated bacteriuria, chlamydial infection and peritonitis/sepsis.
For research use only. We do not sell to patients.
- Purity : 98.20%
- CAS No.: 32887-08-4
- Formula: C15H24ClN3O3S
- Molecular Weight:361.89
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Mecillinam hydrochloride
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Biological Activity
Description
IC50 & Target
[6]|
β-lactam |
In Vitro
Mecillinam (0.4-100 μg/mL; 18 h) hydrochloride inhibits a significant proportion of Gram-negative isolates by the agar dilution method, with 75% of Escherichia coli and 62% of Enterobacter inhibited at 6.3 μg/mL[2].
Mecillinam (0.009-10 mg/L; 18 h) hydrochloride inhibits E. coli O54 with an MIC of 10 mg/L and produces complete growth inhibition when used in combination at 0.156 mg/L (FIC 0.016), with a total FIC of 0.046[4].
Mecillinam (0.0625-32 mg/L; 30 min) hydrochloride binds PBP2 with an IC50 of 0.3 mg/L in the E. coli MG1655 PBP competition assay, and Avibactam (HY-14879) does not alter the PBP binding affinity of Mecillinam[5].
Mecillinam (4 mg/L) hydrochloride combined with 1 mg/L Avibactam effectively kills K. pneumoniae isolates KP2, KP5, KP6, KP7, KP8, and ATCC 13883[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Mecillinam (30 mg/kg alone; 2-8 mg/kg in combination with ampicillin; subcutaneous injection; single injection) hydrochloride is ineffective against normal mice with E. coli thigh infection, but low doses strongly enhance the antibacterial activity of Ampicillin (HY-B0522)[4].
Mecillinam (30 mg/kg alone; 2-8 mg/kg in combination with Ampicillin; subcutaneous injection; single injection) hydrochloride is ineffective against Escherichia coli thigh infection in irradiated granulocytopenic mice, but low doses still enhance the antibacterial activity of Ampicillin[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:New Zealand white (male)[1]
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Dosage:50, 100, 200, or 400 mg/kg body weight each day
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Administration:intravenous; four divided doses each day; 72 h
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Result:At sacrifice, log10 cfu of E. coli/mL or cm2 in urethral catheter were NG for 400 mg/kg, 2.02 for 200 mg/kg (2/6), 0.97 for 100 mg/kg (2/5), and 2.53 for 50 mg/kg (2/5).
At 400 mg/kg, at 48 h four of five rabbits had completely eliminated bacteria from urine; at 72 h all rabbits had sterile catheter urine, and urine, catheter, and bladder wall samples were free of detectable bacteria.
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Animal Model:Swiss-type (SPF, male, 20-25 g)[4]
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Dosage:30 mg/kg (alone); 2 mg/kg (with ampicillin); 4 mg/kg (with ampicillin); 8 mg/kg (with ampicillin)
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Administration:subcutaneous; single injection
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Result:At 30 mg/kg alone, did not produce an antibacterial effect in normal mice.
In combination with ampicillin, produced a strong potentiating effect.
The lowest dose examined in combination was 2 mg/kg.
Potency ratios of the combinations relative to ampicillin alone, plotted against dose in Fig. 5a, increased with dose (2, 4, and 8 mg/kg).
Chemical Information
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CAS No. 32887-08-4
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Appearance Solid
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Molecular Weight 361.89
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Formula C15H24ClN3O3S
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Color White to off-white
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SMILES
O=C([C@@H](C(C)(C)S[C@]1([H])[C@@H]2/N=C/N3CCCCCC3)N1C2=O)O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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J Antimicrob Chemother
Unravelling the triad of penicillin-binding proteins, β-lactamase activity, and mRNA dynamics in Pseudomonas aeruginosa AmpC induction. [Abstract]2025 Nov 5:dkaf408. PMID: 41206063 -
Microbiol Spectr
Penicillin-Binding Protein Occupancy Dataset for 18 β-Lactams and 4 β-Lactamase Inhibitors in Neisseria gonorrhoeae. [Abstract]2023 Jun 15;11(3):e0069223. PMID: 37093051 -
Microbiol Spectr
PBP Target Profiling by β-Lactam and β-Lactamase Inhibitors in Intact Pseudomonas aeruginosa: Effects of the Intrinsic and Acquired Resistance Determinants on the Periplasmic Drug Availability. [Abstract]2023 Feb 14;11(1):e0303822. PMID: 36475840 -
J Glob Antimicrob Resist
Susceptibility of multidrug-resistant Escherichia coli and Klebsiella pneumoniae to oral antibiotics in Australia. [Abstract]2025 Aug 8:44:435-441. PMID: 40784378
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (138.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (296 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Olson ME, et al. Amdinocillin treatment of catheter-associated bacteriuria in rabbits. The Journal of infectious diseases. 1989 Jun;159(6):1065-72. [Content Brief]
[2]. Neu HC, et al. Mecillinam, a novel penicillanic acid derivative with unusual activity against gram-negative bacteria. Antimicrobial agents and chemotherapy. 1976 May;9(5):793-9. [Content Brief]
[5]. List KK, et al. Synergy between Mecillinam and Ceftazidime/Avibactam or Avibactam against Multi-Drug-Resistant Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae. Antibiotics (Basel). 2022 Sep 20;11(10):1280. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7633 mL | 13.8164 mL | 27.6327 mL | 69.0818 mL |
| 5 mM | 0.5527 mL | 2.7633 mL | 5.5265 mL | 13.8164 mL | |
| 10 mM | 0.2763 mL | 1.3816 mL | 2.7633 mL | 6.9082 mL | |
| 15 mM | 0.1842 mL | 0.9211 mL | 1.8422 mL | 4.6055 mL | |
| 20 mM | 0.1382 mL | 0.6908 mL | 1.3816 mL | 3.4541 mL | |
| 25 mM | 0.1105 mL | 0.5527 mL | 1.1053 mL | 2.7633 mL | |
| 30 mM | 0.0921 mL | 0.4605 mL | 0.9211 mL | 2.3027 mL | |
| 40 mM | 0.0691 mL | 0.3454 mL | 0.6908 mL | 1.7270 mL | |
| 50 mM | 0.0553 mL | 0.2763 mL | 0.5527 mL | 1.3816 mL | |
| 60 mM | 0.0461 mL | 0.2303 mL | 0.4605 mL | 1.1514 mL | |
| 80 mM | 0.0345 mL | 0.1727 mL | 0.3454 mL | 0.8635 mL | |
| 100 mM | 0.0276 mL | 0.1382 mL | 0.2763 mL | 0.6908 mL |
Mecillinam hydrochloride Related Classifications
HY-A0269A Related Classifications
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