Myelin basic protein (4-14) TFA
Based on 2 publication(s) in Google Scholar
Myelin Basic Protein (MHP4-14) TFA, a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (Km=7 μM). Myelin Basic Protein TFA is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts.
For research use only. We do not sell to patients.
- Purity: 99.41%
- Formula: C62H104F3N21O19
- Molecular Weight:1504.61
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Myelin basic protein (4-14) TFA
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Biological Activity
Once MBP4-14 is phosphorylated, it is not dephosphorylated by okadaic acid-sensitive phosphatases (protein phosphatases 1, 2A and 3) or other protein phosphatases such as calcineurin and/or PP 2C present in hippocampal homogenates. Therefore, MBP4-14 can be used for PKC assay in crude extracts of neural tissue[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1504.61
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Formula C62H104F3N21O19
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Color White to off-white
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Synonyms
MHP4-14 TFA
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Sequence Shortening
QKRPSQRSKYL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Autophagy
The role of the key autophagy kinase ULK1 in hepatocellular carcinoma and its validation as a treatment target. [Abstract]2020 Oct;16(10):1823-1837. PMID: 31986961 -
Heliyon
Structure-based virtual screening of natural compounds in preventing skin senescence: The role of epigallocatechin gallate in protein kinase C alpha-specific inhibition against UV-induced photoaging. [Abstract]2024 Oct 30;10(21):e39933. PMID: 39553571
Solvent & Solubility
H2O : ≥ 100 mg/mL (66.46 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (66.46 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Farrar YJ, et al. A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts. Biochem Biophys Res Commun. 1991;180(2):694-701. [Content Brief]
[2]. Yasuda I, et al. A synthetic peptide substrate for selective assay of protein kinase C. Biochem Biophys Res Commun. 1990;166(3):1220-1227. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.6646 mL | 3.3231 mL | 6.6462 mL | 16.6156 mL |
| 5 mM | 0.1329 mL | 0.6646 mL | 1.3292 mL | 3.3231 mL | |
| 10 mM | 0.0665 mL | 0.3323 mL | 0.6646 mL | 1.6616 mL | |
| 15 mM | 0.0443 mL | 0.2215 mL | 0.4431 mL | 1.1077 mL | |
| 20 mM | 0.0332 mL | 0.1662 mL | 0.3323 mL | 0.8308 mL | |
| 25 mM | 0.0266 mL | 0.1329 mL | 0.2658 mL | 0.6646 mL | |
| 30 mM | 0.0222 mL | 0.1108 mL | 0.2215 mL | 0.5539 mL | |
| 40 mM | 0.0166 mL | 0.0831 mL | 0.1662 mL | 0.4154 mL | |
| 50 mM | 0.0133 mL | 0.0665 mL | 0.1329 mL | 0.3323 mL | |
| 60 mM | 0.0111 mL | 0.0554 mL | 0.1108 mL | 0.2769 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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