Negletein
Based on 1 Customer Validation
Negletein (5,6-Dihydroxy-7-methoxyflavone) is a flavone found in Scutellaria. Negletein shows anti-inflammatory activity via inhibiting TNF-α and IL-1β with IC50 values of 16.4 and 6.4 μM, respectively. Negletein can activate Nrf2 and inhibit ROS production. Negletein can enhance the neuroprotective effect of nerve growth factor. Negletein can inhibit amyloid beta-peptide release and accumulation. Negletein can inhibit pathogens biofilms formation. Negletein can be used for the researches of cancer, infection, inflammation and neurological disease, such as colon cancer and Alzheimer's disease.
For research use only. We do not sell to patients.
- Purity: 99.55%
- CAS No.: 29550-13-8
- Formula: C16H12O5
- Molecular Weight:284.26
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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IL-1β 6.4 nM (IC50) |
TNF-α 16.4 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DLD-1 | IC50 |
30.93 μM
Compound: 5a
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Antiproliferative activity against human DLD1 cells after 48 hrs by MTT assay
Antiproliferative activity against human DLD1 cells after 48 hrs by MTT assay
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10.1039/C5MD00163C |
| H9 | IC50 |
11.5 μg/mL
Compound: 2
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Concentration that inhibits uninfected H9 cell growth by 50%.
Concentration that inhibits uninfected H9 cell growth by 50%.
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[PMID: 12729671] |
| HepG2 | IC50 |
>40 μM
Compound: 5a
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Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
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10.1039/C5MD00163C |
| HT-29 | IC50 |
>40 μM
Compound: 5a
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Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
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10.1039/C5MD00163C |
| J774.A1 | IC50 |
0.22 μM
Compound: 47
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Anti-inflammatory activity in mouse J774.A1 macrophage cells assessed as reduction in LPS-induced TNF-alpha production
Anti-inflammatory activity in mouse J774.A1 macrophage cells assessed as reduction in LPS-induced TNF-alpha production
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[PMID: 37683361] |
| J774.A1 | IC50 |
6.4 μM
Compound: 47
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Antiinflammatory activity in LPS-induced mouse J774.A1 macrophage cells assessed as reduction in LPS-induced IL-1 beta level
Antiinflammatory activity in LPS-induced mouse J774.A1 macrophage cells assessed as reduction in LPS-induced IL-1 beta level
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[PMID: 37683361] |
| SW480 | IC50 |
29.41 μM
Compound: 5a
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Antiproliferative activity against human SW480 cells after 48 hrs by MTT assay
Antiproliferative activity against human SW480 cells after 48 hrs by MTT assay
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10.1039/C5MD00163C |
Negletein (10 nM-10 mM) does not induce neurite outgrowth in PC12 cells[1].
Negletein (10 μM, 3 days) trigger PC12 cells to differentiate and extend neuritis companied with nerve growth factor (5 ng/mL)[1].
Negletein (10 μM) increases p-ERK, p-Akt, p-CREB, and GAP-43 levels companied with nerve growth factor (5 ng/mL) in PC12 cells[1].
Negletein (10 nM-10 μM, 24-72 h) increases survival rate (cell viability) of PC12 cells cultured in serum-free medium[1].
Negletein (20 μM) inhibits APP expression, β-Secretase activity and Aβ42 release in iron-induced SHSY5Y cells[3].
Negletein (5-20 μM) inhibits hydroxyl radical formation in iron-induced SHSY5Y cells[3].
Negletein (20 μM, 24 h) prevents oligomerization of amyloid Aβ42 in iron-induced SHSY5Y cells[3].
Negletein (5-50 μM, 4 h) increases Nrf2 expression in HCT116 cells[4].
Negletein (0-30 μM, 4 h) reduces ROS production in HCT116 cells[4].
Negletein shows MIC of 32 μg/mL against S. aureus and Bacillus sp.[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 29550-13-8
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Appearance Solid
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Molecular Weight 284.26
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Formula C16H12O5
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Color Light yellow to green yellow
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SMILES
O=C1C=C(C2=CC=CC=C2)OC3=CC(OC)=C(O)C(O)=C13
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Synonyms
5,6-Dihydroxy-7-methoxyflavone
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (351.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Phan CW, et al. Negletein as a neuroprotectant enhances the action of nerve growth factor and induces neurite outgrowth in PC12 cells. Biofactors. 2016 Nov 12;42(6):591-599. [Content Brief]
[2]. Singh B, et al. Anti-inflammatory and immunomodulatory flavones from Actinocarya tibetica Benth. Nat Prod Res. 2013;27(23):2227-30. [Content Brief]
[3]. Banerjee P, et al. Multiple mechanisms of iron-induced amyloid beta-peptide accumulation in SHSY5Y cells: protective action of negletein. Neuromolecular Med. 2014 Dec;16(4):787-98. [Content Brief]
[4]. Havermann S, et al. Modulation of the Nrf2 signalling pathway in Hct116 colon carcinoma cells by baicalein and its methylated derivative negletein. Pharm Biol. 2016 Sep;54(9):1491-502. [Content Brief]
[5]. Rajendran N, et al. Antimicrobial flavonoids isolated from Indian medicinal plant Scutellaria oblonga inhibit biofilms formed by common food pathogens. Nat Prod Res. 2016 Sep;30(17):2002-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5179 mL | 17.5895 mL | 35.1791 mL | 87.9477 mL |
| 5 mM | 0.7036 mL | 3.5179 mL | 7.0358 mL | 17.5895 mL | |
| 10 mM | 0.3518 mL | 1.7590 mL | 3.5179 mL | 8.7948 mL | |
| 15 mM | 0.2345 mL | 1.1726 mL | 2.3453 mL | 5.8632 mL | |
| 20 mM | 0.1759 mL | 0.8795 mL | 1.7590 mL | 4.3974 mL | |
| 25 mM | 0.1407 mL | 0.7036 mL | 1.4072 mL | 3.5179 mL | |
| 30 mM | 0.1173 mL | 0.5863 mL | 1.1726 mL | 2.9316 mL | |
| 40 mM | 0.0879 mL | 0.4397 mL | 0.8795 mL | 2.1987 mL | |
| 50 mM | 0.0704 mL | 0.3518 mL | 0.7036 mL | 1.7590 mL | |
| 60 mM | 0.0586 mL | 0.2932 mL | 0.5863 mL | 1.4658 mL | |
| 80 mM | 0.0440 mL | 0.2199 mL | 0.4397 mL | 1.0993 mL | |
| 100 mM | 0.0352 mL | 0.1759 mL | 0.3518 mL | 0.8795 mL |
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