Neplanocin A (Synonyms: (-)-Neplanocin A)

Name: Neplanocin A
Brand: MedChemExpress
SKU: HY-130430
Rating: 4.5 (1 reviews)

Cat. No.: HY-130430 Purity: 99.76%: Data Sheet
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Neplanocin A ((-)-Neplanocin A) is an antitumor antibiotic with significant antitumor activity against murine L1210 leukemia. Neplanocin A is also an irreversible inhibitor of AdoHcy hydrolase (K_i=8.39 nM). Neplanocin A also has antiviral activity and is effective against vaccinia virus. Neplanocin A is obtained from Ampulariella regularis.

For research use only. We do not sell to patients.

Neplanocin A Chemical Structure

CAS No. : 72877-50-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 μg	In-stock	Estimated Time of Arrival: December 31
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

AdoHcy hydrolase; vaccinia^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.22 μM Compound: 1	Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
A549	IC₅₀	2.16 μM Compound: Neplanocin A; 1a	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human A549 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
CCRF-CEM	IC₅₀	1.12 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in CEM cells Antiviral and cytostatic effects againstTK-VZV virus expressed in CEM cells	[PMID: 8809173]
CCRF-CEM	IC₅₀	1.2 μM Compound: 1	In vitro cytotoxicity in CEM cells. In vitro cytotoxicity in CEM cells.	[PMID: 11689085]
E6SM	CC₅₀	0.7 μg/mL Compound: NPA	Cytotoxic activity against vaccinia virus in E6SM Cells Cytotoxic activity against vaccinia virus in E6SM Cells	[PMID: 8691433]
E6SM	CC₅₀	40 μg/mL Compound: NPA	Cytotoxic activity against TK-HSV-1 (B2006) virus in E6SM Cells Cytotoxic activity against TK-HSV-1 (B2006) virus in E6SM Cells	[PMID: 8691433]
E6SM	CC₅₀	70 μg/mL Compound: NPA	Cytotoxic activity against cell morphology in E6SM Cells Cytotoxic activity against cell morphology in E6SM Cells	[PMID: 8691433]
E6SM	CC₅₀	7 μg/mL Compound: NPA	Cytotoxic activity against VSV virus in E6SM Cells Cytotoxic activity against VSV virus in E6SM Cells	[PMID: 8691433]
E6SM	CC₅₀	> 70 μg/mL Compound: NPA	Cytotoxic activity against HSV-1(KOS) virus in E6SM Cells Cytotoxic activity against HSV-1(KOS) virus in E6SM Cells	[PMID: 8691433]
E6SM	CC₅₀	> 70 μg/mL Compound: NPA	Cytotoxic activity against HSV-2 (G) virus in E6SM Cells Cytotoxic activity against HSV-2 (G) virus in E6SM Cells	[PMID: 8691433]
E6SM	IC₅₀	0.4 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against CMV (Davis) virus expressed in E6SM HEL cells Antiviral and cytostatic effects against CMV (Davis) virus expressed in E6SM HEL cells	[PMID: 8809173]
E6SM	IC₅₀	0.7 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against VV virus expressed in E6SM cells Antiviral and cytostatic effects against VV virus expressed in E6SM cells	[PMID: 8809173]
E6SM	IC₅₀	20 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against TK HSV-1( B2006) virus expressed in E6SM cells Antiviral and cytostatic effects against TK HSV-1( B2006) virus expressed in E6SM cells	[PMID: 8809173]
E6SM	IC₅₀	70 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Hsv-1(KOS) virus expressed in E6SM cells Antiviral and cytostatic effects against Hsv-1(KOS) virus expressed in E6SM cells	[PMID: 8809173]
E6SM	IC₅₀	70 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in E6SM cells Antiviral and cytostatic effects againstTK-VZV virus expressed in E6SM cells	[PMID: 8809173]
E6SM	IC₅₀	7 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against VSV virus expressed in E6SM cells Antiviral and cytostatic effects against VSV virus expressed in E6SM cells	[PMID: 8809173]
E6SM	IC₅₀	> 40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Hsv-2(G) virus expressed in E6SM cells Antiviral and cytostatic effects against Hsv-2(G) virus expressed in E6SM cells	[PMID: 8809173]
ESM	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus VV on ESM cell line. Inhibitory effect on replication of DNA virus VV on ESM cell line.	[PMID: 1732550]
ESM	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus VSV in ESM cell line. Inhibitory effect on replication of RNA virus VSV in ESM cell line.	[PMID: 1732550]
ESM	IC₅₀	10 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus HSV-2 (G) in ESM cell line Inhibitory effect on replication of DNA virus HSV-2 (G) in ESM cell line	[PMID: 1732550]
ESM	IC₅₀	20 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus HSV-1 (KOS) in ESM cell line Inhibitory effect on replication of DNA virus HSV-1 (KOS) in ESM cell line	[PMID: 1732550]
ESM	IC₅₀	40 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus Cell morphology on ESM cell line Inhibitory effect on replication of DNA virus Cell morphology on ESM cell line	[PMID: 1732550]
ESM	IC₅₀	40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus cell morphology in ESM cell line. Inhibitory effect on replication of RNA virus cell morphology in ESM cell line.	[PMID: 1732550]
ESM	IC₅₀	4 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus TK-HSV-1 (B2006) on ESM cell line. Inhibitory effect on replication of DNA virus TK-HSV-1 (B2006) on ESM cell line.	[PMID: 1732550]
ESM	IC₅₀	7 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus TK-HSV-1 (VMW 1837) on ESM cell line. Inhibitory effect on replication of DNA virus TK-HSV-1 (VMW 1837) on ESM cell line.	[PMID: 1732550]
FM3A	IC₅₀	0.022 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in FM3A cells Antiviral and cytostatic effects againstTK-VZV virus expressed in FM3A cells	[PMID: 8809173]
HCT-116	IC₅₀	0.9 μM Compound: 1	Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
HCT-116	IC₅₀	1.45 μM Compound: Neplanocin A; 1a	Cytotoxicity against human HCT116 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human HCT116 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
HEL	CC₅₀	0.3 μg/mL Compound: NPA	Cytotoxic activity against HCMV (AD-169) virus in HEL Cells Cytotoxic activity against HCMV (AD-169) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	0.4 μg/mL Compound: NPA	Cytotoxic activity against HCMV(Davis) virus in HEL Cells Cytotoxic activity against HCMV(Davis) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	10 μg/mL Compound: NPA	Cytotoxic activity against TK-VZV (07/1) virus in HEL Cells Cytotoxic activity against TK-VZV (07/1) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	15 μg/mL Compound: NPA	Cytotoxic activity against VZV (OKA) virus in HEL Cells Cytotoxic activity against VZV (OKA) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	7 μg/mL Compound: NPA	Cytotoxic activity against TK-VZV (YS/R) virus in HEL Cells Cytotoxic activity against TK-VZV (YS/R) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	9 μg/mL Compound: NPA	Cytotoxic activity against VZV (YS) virus in HEL Cells Cytotoxic activity against VZV (YS) virus in HEL Cells	[PMID: 8691433]
HEL	CC₅₀	> 20 μg/mL Compound: NPA	Cytotoxic activity against cell growth in HEL Cells Cytotoxic activity against cell growth in HEL Cells	[PMID: 8691433]
HEL	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus CMV (Davis) on HEL cell line. Inhibitory effect on replication of DNA virus CMV (Davis) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	0.3 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against CMV (AD-169) virus expressed in HEL cells Antiviral and cytostatic effects against CMV (AD-169) virus expressed in HEL cells	[PMID: 8809173]
HEL	IC₅₀	0.3 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus CMV (AD-169) on HEL cell line. Inhibitory effect on replication of DNA virus CMV (AD-169) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	10 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against VZV(Oka,YS) virus expressed in HEL cells Antiviral and cytostatic effects against VZV(Oka,YS) virus expressed in HEL cells	[PMID: 8809173]
HEL	IC₅₀	16 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus Cell growth on HEL cell line. Inhibitory effect on replication of DNA virus Cell growth on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	2 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus TK-VZV (YSR) on HEL cell line. Inhibitory effect on replication of DNA virus TK-VZV (YSR) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	5 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus TK-VZV (07-1) on HEL cell line. Inhibitory effect on replication of DNA virus TK-VZV (07-1) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	5 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus VZV (YS) on HEL cell line. Inhibitory effect on replication of DNA virus VZV (YS) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	8 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus VZV (Oka) on HEL cell line. Inhibitory effect on replication of DNA virus VZV (Oka) on HEL cell line.	[PMID: 1732550]
HEL	IC₅₀	9 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against TK-VZV virus expressed in HEL cells Antiviral and cytostatic effects against TK-VZV virus expressed in HEL cells	[PMID: 8809173]
HEL	IC₅₀	> 20 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in HEL cells Antiviral and cytostatic effects againstTK-VZV virus expressed in HEL cells	[PMID: 8809173]
HeLa	CC₅₀	2 μg/mL Compound: NPA	Cytotoxic activity against VZV virus in HeLa Cells Cytotoxic activity against VZV virus in HeLa Cells	[PMID: 8691433]
HeLa	CC₅₀	> 40 μg/mL Compound: NPA	Cytotoxic activity against Coxsackie B4 virus in HeLa Cells Cytotoxic activity against Coxsackie B4 virus in HeLa Cells	[PMID: 8691433]
HeLa	CC₅₀	> 40 μg/mL Compound: NPA	Cytotoxic activity against Polio-1 virus in HeLa Cells Cytotoxic activity against Polio-1 virus in HeLa Cells	[PMID: 8691433]
HeLa	CC₅₀	> 40 μg/mL Compound: NPA	Cytotoxic activity against cell morphology in HeLa Cells Cytotoxic activity against cell morphology in HeLa Cells	[PMID: 8691433]
HeLa	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus RSV in HeLa cell line Inhibitory effect on replication of RNA virus RSV in HeLa cell line	[PMID: 1732550]
HeLa	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus VSV in HeLa cell line. Inhibitory effect on replication of RNA virus VSV in HeLa cell line.	[PMID: 1732550]
HeLa	IC₅₀	20 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus RNA virus cell morphology in HeLa cell line. Inhibitory effect on replication of DNA virus RNA virus cell morphology in HeLa cell line.	[PMID: 1732550]
HeLa	IC₅₀	2 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against VSV virus expressed in Hela cells Antiviral and cytostatic effects against VSV virus expressed in Hela cells	[PMID: 8809173]
HeLa	IC₅₀	40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in Hela cells Antiviral and cytostatic effects againstTK-VZV virus expressed in Hela cells	[PMID: 8809173]
HeLa	IC₅₀	> 40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Coxsackie B4 virus expressed in HeLa cells Antiviral and cytostatic effects against Coxsackie B4 virus expressed in HeLa cells	[PMID: 8809173]
HeLa	IC₅₀	> 40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Polio-1 virus expressed in Hela cells Antiviral and cytostatic effects against Polio-1 virus expressed in Hela cells	[PMID: 8809173]
HeLa	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus POLIO-1 on HeLa cell line. Inhibitory effect on replication of RNA virus POLIO-1 on HeLa cell line.	[PMID: 1732550]
HeLa	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus coxsB-4 in HeLa cell line. Inhibitory effect on replication of RNA virus coxsB-4 in HeLa cell line.	[PMID: 1732550]
L1210	IC₅₀	0.027 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in L1210 cells Antiviral and cytostatic effects againstTK-VZV virus expressed in L1210 cells	[PMID: 8809173]
L929	IC₅₀	0.0052 μM Compound: Neplanocin A	Inhibitory concentration on L929 cells Inhibitory concentration on L929 cells	[PMID: 8120872]
L929	IC₅₀	0.05 μM Compound: Ncp A	Evaluated for the 50% inhibition of S-Adenosyl-homocysteine (AdoHcy) hydrolase L929 lysate from murine L-929 cells Evaluated for the 50% inhibition of S-Adenosyl-homocysteine (AdoHcy) hydrolase L929 lysate from murine L-929 cells	[PMID: 3411600]
L929	IC₅₀	0.08 μM Compound: Ncp A	Evaluated for 50% plaque inhibition of vaccinia virus replication in murine L929 cells Evaluated for 50% plaque inhibition of vaccinia virus replication in murine L929 cells	[PMID: 3411600]
MCF7	IC₅₀	< 25 μM Compound: 11, Nep A	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 26314922]
MDA-MB-231	IC₅₀	0.4 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
MDA-MB-231	IC₅₀	0.86 μM Compound: Neplanocin A; 1a	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
MDA-MB-231	IC₅₀	< 25 μM Compound: 11, Nep A	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 26314922]
MDCK	IC₅₀	20 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus cell morphology in MDCK cell line. Inhibitory effect on replication of RNA virus cell morphology in MDCK cell line.	[PMID: 1732550]
MDCK	IC₅₀	> 20 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus influenza A in MDCK cell line. Inhibitory effect on replication of RNA virus influenza A in MDCK cell line.	[PMID: 1732550]
MDCK	IC₅₀	> 20 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus influenza B in MDCK cell line. Inhibitory effect on replication of RNA virus influenza B in MDCK cell line.	[PMID: 1732550]
MOLT-4	IC₅₀	2.34 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in MOLT-4 cells Antiviral and cytostatic effects againstTK-VZV virus expressed in MOLT-4 cells	[PMID: 8809173]
MT4	IC₅₀	0.02 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus cell growth in MT-4 cell line. Inhibitory effect on replication of RNA virus cell growth in MT-4 cell line.	[PMID: 1732550]
MT4	IC₅₀	> 0.006 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus HIV-1 in MT-4 cell line. Inhibitory effect on replication of RNA virus HIV-1 in MT-4 cell line.	[PMID: 1732550]
PC-3	IC₅₀	2.14 μM Compound: Neplanocin A; 1a	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
PC-3	IC₅₀	3.7 μM Compound: 1	Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
PRK	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus VV on PRK cell line. Inhibitory effect on replication of DNA virus VV on PRK cell line.	[PMID: 1732550]
PRK	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus VSV in PRK cell line. Inhibitory effect on replication of RNA virus VSV in PRK cell line.	[PMID: 1732550]
PRK	IC₅₀	40 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus Cell morphology on PRK cell line Inhibitory effect on replication of DNA virus Cell morphology on PRK cell line	[PMID: 1732550]
PRK	IC₅₀	40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus cell morphology in PRK cell line. Inhibitory effect on replication of RNA virus cell morphology in PRK cell line.	[PMID: 1732550]
PRK	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus HSV-1 (KOS) in PRK cell line. Inhibitory effect on replication of DNA virus HSV-1 (KOS) in PRK cell line.	[PMID: 1732550]
PRK	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of DNA virus HSV-2 (G) in PRK cell line. Inhibitory effect on replication of DNA virus HSV-2 (G) in PRK cell line.	[PMID: 1732550]
SH-SY5Y	IC₅₀	10 nM Compound: 8, NepA	Inhibition of AHCY in human SH-SY5Y cells assessed as formation of homocysteine after 48 hrs by HPLC analysis Inhibition of AHCY in human SH-SY5Y cells assessed as formation of homocysteine after 48 hrs by HPLC analysis	[PMID: 24813734]
SK-BR-3	IC₅₀	< 25 μM Compound: 11, Nep A	Cytotoxicity against human SKBR3 cells Cytotoxicity against human SKBR3 cells	[PMID: 26314922]
SK-HEP1	IC₅₀	2.36 μM Compound: Neplanocin A; 1a	Cytotoxicity against human SKHEP1 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human SKHEP1 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
SK-HEP1	IC₅₀	4.5 μM Compound: 1	Cytotoxicity against human SKHEP1 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human SKHEP1 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
SNU-638	IC₅₀	0.7 μM Compound: 1	Cytotoxicity against human SNU638 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay Cytotoxicity against human SNU638 cells assessed as inhibition of cell proliferation after 72 hrs by sulforhodamine B assay	[PMID: 26010585]
SNU-638	IC₅₀	0.82 μM Compound: Neplanocin A; 1a	Cytotoxicity against human SNU638 cells assessed as decrease in cell viability after 72 hrs by SRB assay Cytotoxicity against human SNU638 cells assessed as decrease in cell viability after 72 hrs by SRB assay	[PMID: 29906687]
Vero	CC₅₀	0.5 μg/mL Compound: NPA	Cytotoxic activity against Junin virus in Vero Cells Cytotoxic activity against Junin virus in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	152 μg/mL Compound: NPA	Cytotoxic activity towards viable cells in Vero cells Cytotoxic activity towards viable cells in Vero cells	[PMID: 8691433]
Vero	CC₅₀	2 μg/mL Compound: NPA	Cytotoxic activity against PV-3 virus in Vero Cells Cytotoxic activity against PV-3 virus in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	2 μg/mL Compound: NPA	Cytotoxic activity against Tacaribe virus in Vero Cells Cytotoxic activity against Tacaribe virus in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	4 μg/mL Compound: NPA	Cytotoxic activity against Reo-1 virus in Vero Cells Cytotoxic activity against Reo-1 virus in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	> 10 μg/mL Compound: NPA	Cytotoxic activity against Sindbis virus in Vero Cells Cytotoxic activity against Sindbis virus in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	> 10 μg/mL Compound: NPA	Cytotoxic activity against cell morphology in Vero Cells Cytotoxic activity against cell morphology in Vero Cells	[PMID: 8691433]
Vero	CC₅₀	> 10 μg/mL Compound: NPA	Cytotoxic activity against forest virus in Vero Cells Cytotoxic activity against forest virus in Vero Cells	[PMID: 8691433]
Vero	EC₅₀	0.03 μg/mL Compound: 2/Neplanocin A	Antiviral activity was measured against Smallpox virus 7124 in Vero 76 (ATCC CRL 1587) cell Antiviral activity was measured against Smallpox virus 7124 in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	EC₅₀	0.14 μg/mL Compound: 2/Neplanocin A	Antiviral activity was measured against Smallpox virus BSH in Vero 76 (ATCC CRL 1587) cell Antiviral activity was measured against Smallpox virus BSH in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	EC₅₀	0.21 μg/mL Compound: 2/Neplanocin A	Antiviral activity was measured against Monkeypox virus MPX in Vero 76 (ATCC CRL 1587) cell Antiviral activity was measured against Monkeypox virus MPX in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	EC₅₀	> 100 μg/mL Compound: 2/Neplanocin A	Antiviral activity was measured against Cowpox virus CPX in Vero 76 (ATCC CRL 1587) cell Antiviral activity was measured against Cowpox virus CPX in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	EC₅₀	> 100 μg/mL Compound: 2/Neplanocin A	Antiviral activity was measured against Vaccinia virus VAC in Vero 76 (ATCC CRL 1587) cell Antiviral activity was measured against Vaccinia virus VAC in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	ED₅₀	0.24 μg/mL Compound: 1 (NPA)	In vitro anti-vesicular stomatitis virus activity in vero cell cultures In vitro anti-vesicular stomatitis virus activity in vero cell cultures	10.1016/S0960-894X(01)80163-3
Vero	IC₅₀	0.21 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus RNA synthesis in Vero cell line. Inhibitory effect on replication of RNA virus RNA synthesis in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.26 μg/mL Compound: 1 (NPA)	Cytotoxic effect on host cells during growth phase of virus in vero cell cultures using MTT method Cytotoxic effect on host cells during growth phase of virus in vero cell cultures using MTT method	10.1016/S0960-894X(01)80163-3
Vero	IC₅₀	0.2 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus measles on Vero cell line. Inhibitory effect on replication of RNA virus measles on Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.4 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus JV in Vero cell line. Inhibitory effect on replication of RNA virus JV in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.4 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus PV-3 in Vero cell line. Inhibitory effect on replication of RNA virus PV-3 in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.4 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus TV in Vero cell line. Inhibitory effect on replication of RNA virus TV in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.4 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus reo-1 in Vero cell line. Inhibitory effect on replication of RNA virus reo-1 in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	0.5 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Junin virus expressed in Vero cells Antiviral and cytostatic effects against Junin virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	0.74 μg/mL Compound: NPA	Inhibitory activity against virus plaque formation (PV-3) in Vero cells Inhibitory activity against virus plaque formation (PV-3) in Vero cells	[PMID: 8691433]
Vero	IC₅₀	0.7 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Reo-1 virus expressed in Vero cells Antiviral and cytostatic effects against Reo-1 virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	1.5 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Tacaribe virus expressed in Vero cells Antiviral and cytostatic effects against Tacaribe virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	10 μg/mL Compound: 2/Neplanocin A	Cytotoxicity was measured against Smallpox virus BSH in Vero 76 (ATCC CRL 1587) cell Cytotoxicity was measured against Smallpox virus BSH in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	IC₅₀	10 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus cell morphology in Vero cell line. Inhibitory effect on replication of RNA virus cell morphology in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	152 μg/mL Compound: 1 (NPA)	Cytotoxic effect on host cells during stationary phase of virus in vero cell cultures using MTT method Cytotoxic effect on host cells during stationary phase of virus in vero cell cultures using MTT method	10.1016/S0960-894X(01)80163-3
Vero	IC₅₀	155 μg/mL Compound: 1a (NPA)	Cytotoxic concentration required to reduce the number of Vero cells by 50% Cytotoxic concentration required to reduce the number of Vero cells by 50%	[PMID: 8809173]
Vero	IC₅₀	1 μg/mL Compound: NPA	Inhibitory activity against virus-induced cytopathogenicity (VSV)in Vero cells Inhibitory activity against virus-induced cytopathogenicity (VSV)in Vero cells	[PMID: 8691433]
Vero	IC₅₀	20 μg/mL Compound: 2/Neplanocin A	Cytotoxicity was measured against Cowpox virus CPX in Vero 76 (ATCC CRL 1587) cell Cytotoxicity was measured against Cowpox virus CPX in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	IC₅₀	2 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Parainfluenza-3 virus expressed in Vero cells Antiviral and cytostatic effects against Parainfluenza-3 virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	3.5 μM Compound: 1	In vitro cytotoxicity in vero cells. In vitro cytotoxicity in vero cells.	[PMID: 11689085]
Vero	IC₅₀	4.3 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus protein synthesis in Vero cell line. Inhibitory effect on replication of RNA virus protein synthesis in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects againstTK-VZV virus expressed in Vero cells Antiviral and cytostatic effects againstTK-VZV virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	50 μg/mL Compound: 2/Neplanocin A	Cytotoxicity was measured against Smallpox virus 7124 in Vero 76 (ATCC CRL 1587) cell Cytotoxicity was measured against Smallpox virus 7124 in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	IC₅₀	57 μg/mL Compound: 2/Neplanocin A	Cytotoxicity was measured against Monkeypox virus MPX in Vero 76 (ATCC CRL 1587) cell Cytotoxicity was measured against Monkeypox virus MPX in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	IC₅₀	580 μM Compound: 1, NPA	The compound was tested for cytotoxicity against monkey kidney cell line ( Vero ). The compound was tested for cytotoxicity against monkey kidney cell line ( Vero ).	[PMID: 11806727]
Vero	IC₅₀	7 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against Sindbis virus expressed in Vero cells Antiviral and cytostatic effects against Sindbis virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	> 100 μg/mL Compound: 2/Neplanocin A	Cytotoxicity was measured against Vaccinia virus VAC in Vero 76 (ATCC CRL 1587) cell Cytotoxicity was measured against Vaccinia virus VAC in Vero 76 (ATCC CRL 1587) cell	[PMID: 12467606]
Vero	IC₅₀	> 40 μg/mL Compound: 1a (NPA)	Antiviral and cytostatic effects against forest virus expressed in Vero cells Antiviral and cytostatic effects against forest virus expressed in Vero cells	[PMID: 8809173]
Vero	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus SFV in Vero cell line Inhibitory effect on replication of RNA virus SFV in Vero cell line	[PMID: 1732550]
Vero	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus SV in Vero cell line. Inhibitory effect on replication of RNA virus SV in Vero cell line.	[PMID: 1732550]
Vero	IC₅₀	> 40 μg/mL Compound: NPA	Inhibitory effect on replication of RNA virus coxsB-4 in Vero cell line Inhibitory effect on replication of RNA virus coxsB-4 in Vero cell line	[PMID: 1732550]

In Vitro

Neplanocin A (1 μM; 0-70 h) inhibits the growth of L929 mouse cells^[1].
Neplanocin A (1 μM; 72 h) inhibits vaccinia virus plaque formation in monolayer cultures of mouse L-cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	L929 mouse cells
Concentration:	1 μM
Incubation Time:	0-70 h
Result:	Slowed the rate of increase in cell number.

Molecular Weight

263.25

Formula

C₁₁H₁₃N₅O₃

CAS No.

72877-50-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O)C(CO)=C[C@H]1N2C=NC3=C(N)N=CN=C23

Structure Classification

Others

Initial Source

Microorganisms

Ampullariella regular

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (63.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7987 mL	18.9934 mL	37.9867 mL
5 mM	0.7597 mL	3.7987 mL	7.5973 mL
10 mM	0.3799 mL	1.8993 mL	3.7987 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (6.34 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (6.34 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (6.34 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (254 KB) HNMR (250 KB) RP-HPLC (276 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Borchardt RT, Keller BT, Patel-Thombre U. Neplanocin A. A potent inhibitor of S-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse L929 cells. J Biol Chem. 1984 Apr 10;259(7):4353-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7987 mL	18.9934 mL	37.9867 mL	94.9668 mL
	5 mM	0.7597 mL	3.7987 mL	7.5973 mL	18.9934 mL
	10 mM	0.3799 mL	1.8993 mL	3.7987 mL	9.4967 mL
	15 mM	0.2532 mL	1.2662 mL	2.5324 mL	6.3311 mL
	20 mM	0.1899 mL	0.9497 mL	1.8993 mL	4.7483 mL
	25 mM	0.1519 mL	0.7597 mL	1.5195 mL	3.7987 mL
	30 mM	0.1266 mL	0.6331 mL	1.2662 mL	3.1656 mL
	40 mM	0.0950 mL	0.4748 mL	0.9497 mL	2.3742 mL
	50 mM	0.0760 mL	0.3799 mL	0.7597 mL	1.8993 mL
	60 mM	0.0633 mL	0.3166 mL	0.6331 mL	1.5828 mL

Neplanocin A Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neplanocin A72877-50-0(-)-Neplanocin AAntibioticOrthopoxvirusvacciniaAmpullariella regulariscancertumorvirusleukemiaInhibitorinhibitorinhibit

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Neplanocin A (Synonyms: (-)-Neplanocin A)

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