2. Metabolic Enzyme/Protease
    GPCR/G Protein
    MAPK/ERK Pathway
    Apoptosis
  3. Tyrosinase
    Ras
    Raf
    MAPKAPK2 (MK2)
    Apoptosis
  4. Norartocarpetin

Norartocarpetin 

Cat. No.: HY-N10503 Purity: 98.92%
COA Handling Instructions

Norartocarpetin is a tyrosinase inhibitor. Norartocarpetin has strong tyrosinase inhibitory activity with an IC50 value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with an IC50 value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediated apoptosis, S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells.

For research use only. We do not sell to patients.

Norartocarpetin Chemical Structure

Norartocarpetin Chemical Structure

CAS No. : 520-30-9

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1 mg USD 140 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Norartocarpetin is a tyrosinase inhibitor. Norartocarpetin has strong tyrosinase inhibitory activity with an IC50 value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with an IC50 value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediated apoptosis, S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells[1][2].

IC50 & Target

IC50: 0.47 μM (tyrosinase);[1] IC50: 22 μM (NCI-H460 cells); 85 μM (MRC-9 cells)[2]
In Vitro

Norartocarpetin has strong mushroom tyrosinase inhibitory activity with an IC50 value of 0.47 μM[1].
Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC50 values of 22 μM and 85 μM, respectively[2].
Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460)[2].
Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest[2].
Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells)
Concentration: 0, 3.12, 6.25, 12.5, 25, 50, and 100 μM
(first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min)
Incubation Time: 48 h
Result: Repressed the viability of NCI-H460 cells in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: MRC-9 and NCI-H460 cells
Concentration: 0, 11, 22, and 44 μM
Incubation Time: 24 h
Result: Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38.

Apoptosis Analysis[2]

Cell Line: MRC-9 and NCI-H460 cells
Concentration: 0. 11, 22 and 44 μM
Incubation Time: 24 h
Result: Led to dose-dependent apoptosis and induced DNA damage.

Cell Cycle Analysis[2]

Cell Line: MRC-9 and NCI-H460 cells
Concentration: 0, 11, 22, and 44 μM
Incubation Time:
Result: Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively.

Cell Invasion AssayMRC-9 and NCI-H460 cells

Cell Line: MRC-9 and NCI-H460 cells
Concentration: 22 μM
Incubation Time: 24 h
Result: Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells.

Cell Migration Assay [2]

Cell Line: MRC-9 and NCI-H460 cells
Concentration: 0, 11, 22, and 44 μM
Incubation Time: 24 h
Result: Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.
Molecular Weight

286.24

Appearance

Solid

Formula

C15H10O6
CAS No.
SMILES

O=C1C=C(C2=CC=C(O)C=C2O)OC3=CC(O)=CC(O)=C13
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (349.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4936 mL 17.4679 mL 34.9357 mL
5 mM 0.6987 mL 3.4936 mL 6.9871 mL
10 mM 0.3494 mL 1.7468 mL 3.4936 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 98.92%

COA (249 KB) HNMR (224 KB) LCMS (143 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Norartocarpetin520-30-9TyrosinaseRasRafMAPKAPK2 (MK2)ApoptosisRaf kinasesMitogen-activated protein kinase activated protein kinase 2MAP kinase activated protein kinase 2MAPK activated protein kinase 2MAPKAP kinase 2tyrosinaseantibrowningNCI-H460 cellsMRC-9 cellslung canceantiproliferativeRas/Raf/MAPK signalingapoptosisS-phase cell cyclemigrationinvasionInhibitorinhibitorinhibit

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