Oudermansin B
Oudermansin B is an antibiotic. Oudermansin B acts on fungi to inhibit protein, RNA and DNA synthesis.
For research use only. We do not sell to patients.
- CAS No.: 87081-56-9
- Formula: C18H23ClO5
- Molecular Weight:354.83
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
0.73 μg/mL
Compound: (-)-oudemansin B
|
Cytotoxicity against human KB cells by resazurin microplate assay
Cytotoxicity against human KB cells by resazurin microplate assay
|
[PMID: 20329738] |
| MCF7 | IC50 |
27.84 μg/mL
Compound: (-)-oudemansin B
|
Cytotoxicity against human MCF7 cells by resazurin microplate assay
Cytotoxicity against human MCF7 cells by resazurin microplate assay
|
[PMID: 20329738] |
| NCI-H187 | IC50 |
0.02 μg/mL
Compound: (-)-oudemansin B
|
Cytotoxicity against human NCI-H187 cells by resazurin microplate assay
Cytotoxicity against human NCI-H187 cells by resazurin microplate assay
|
[PMID: 20329738] |
| Vero | IC50 |
14.27 μg/mL
Compound: (-)-oudemansin B
|
Cytotoxicity against african green monkey Vero cells by green fluorescent protein assay
Cytotoxicity against african green monkey Vero cells by green fluorescent protein assay
|
[PMID: 20329738] |
Chemical Information
-
CAS No. 87081-56-9
-
Molecular Weight 354.83
-
Formula C18H23ClO5
-
SMILES
COC(/C([C@H](C)[C@@H](OC)/C=C/C1=CC(OC)=C(C=C1)Cl)=C/OC)=O
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
-
BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Oudermansin B Related Classifications
HY-N14898 Related Classifications
Powered by Bioz