Cat. No.: HY-N6065

Praeruptorin A

(Synonyms: (+)-Praeruptorin A)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Praeruptorin A ((+)-Praeruptorin A) is an orally active isomer of (±)-Praeruptorin A (HY-N0081). Praeruptorin A also acts as a Calcium channel blocker. Praeruptorin A can be isolated from Peucedanum. Praeruptorin A serves as a substrate for CYP3A4. Praeruptorin A downregulates NMDA receptors containing GluN2B and inhibits neuronal Apoptosis. Praeruptorin A mediates vasodilation, inhibits vascular hypertrophy and reduces blood pressure. Praeruptorin A can be used in research related to neurological diseases, myocardial ischemia, heart failure, exertional angina, renovascular hypertension and spontaneous hypertension.

For research use only. We do not sell to patients.

Purity: 99.57%
CAS No.: 73069-27-9
Formula: C₂₁H₂₂O₇
Molecular Weight:386.40

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Praeruptorin A Chemical Structure

CAS No.: 73069-27-9

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

CYP3A4

GluN2B

All Calcium Channel Isoforms :

All Cytochrome P450 Isoforms :

All iGluR Isoforms :

In Vitro

Praeruptorin A (0.05-30 μM; 20 min) undergoes extensive metabolism via hydrolysis and oxidation in pooled HLM, where the formation of M1 exhibits high affinity (K_m1 = 0.02 μM) with an intrinsic clearance of 1.29 mL/min/mg protein^[1].
The metabolism of Praeruptorin A (10 μM; 20 min) is catalyzed by multiple recombinant CYP isoenzymes, among which CYP3A4 exhibits the highest total activity^[1].
Praeruptorin A (10 μM) protects primary cortical neurons against NMDA-induced excitotoxic injury by inhibiting the expression of GluN2B-containing NMDA receptors and regulating the Bcl-2 family^[2].
Praeruptorin A exhibits cytotoxicity against P-388 lymphocytic leukemia cells, with an ED₅₀ of 2.6 pg/mL^[2].
Praeruptorin A exhibits stereoselective metabolism in rat liver microsomes, producing 12 metabolites via hydrolysis, oxidation and acyl migration in the presence of the NADPH regeneration system^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

(+)-Praeruptorin A (2 mg/kg; p.o.; single dose) and (+)-Praeruptorin A (20-100 μg/kg; i.v.; single dose) exerts cardioprotective and vasorelaxant effects, including reversing vascular hypertrophy in hypertensive rats, reducing blood pressure in normotensive and hypertensive rats, and lowering vascular resistance in dogs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Spontaneously hypertensive rats; renovascular hypertensive rats; normotensive rats; anesthetized dogs^[2]
Dosage:

2 mg/kg (blood pressure reduction); 20 μg/kg (vascular resistance reduction); 100 μg/kg (vascular resistance reduction)
Administration:

p.o. (single dose; blood pressure reduction); i.v. (single dose; vascular resistance reduction)
Result:

Recovered vascular hypertrophy in renovascular and spontaneously hypertensive rats by decreasing smooth muscle cell area, collagen content, and intracellular calcium, and increasing nitric oxide production.
Reduced blood pressure in conscious normotensive and renal hypertensive rats at 2 mg/kg p.o.
Decreased vertebral, left circumflex coronary, and femoral vascular resistance in anesthetized dogs at 20 and 100 μg/kg i.v.

Chemical Information

CAS No. 73069-27-9
Appearance Solid
Molecular Weight 386.40
Formula C₂₁H₂₂O₇
Color White to off-white
SMILES

C/C=C(C)\C(O[C@H]1[C@@H](OC(C)=O)C2=C3C(C=CC(O3)=O)=CC=C2OC1(C)C)=O
Synonyms

(+)-Praeruptorin A
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
- Plants
- Umbelliferae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

Eur J Med Chem

Praeruptorin A screened by a ferrous ion probe inhibited DMT1 and ferroptosis to attenuate Doxorubicin-induced cardiomyopathy. [Abstract]

2025 Feb 5:283:117108. PMID: 39615370
Chem Biol Drug Des

Investigation Into the Inhibition of Ferroptosis by Praeruptorin-A in Sepsis Based on Network Pharmacology. [Abstract]

2025 Nov;106(5):e70196. PMID: 41195620
Chem Biol Drug Des

Praeruptorin A inhibits the activation of NF-κB pathway and the expressions of inflammatory factors in poly (I:C)-induced RAW264.7 cells. [Abstract]

2023 Nov;102(5):1110-1120. PMID: 37500542

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (86.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5880 mL

12.9400 mL

25.8799 mL

5 mM

0.5176 mL

2.5880 mL

5.1760 mL

10 mM

0.2588 mL

1.2940 mL

2.5880 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (6.47 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jing WH, et al. Identification of cytochrome P450 isoenzymes involved in metabolism of (+)-praeruptorin A, a calcium channel blocker, by human liver microsomes using ultra high-performance liquid chromatography coupled with tandem mass spectrometry. J Pharm Biomed Anal. 2013;77:175-188. [Content Brief]

[2]. Sarkhail P, et al. Biological activities and pharmacokinetics of praeruptorins from Peucedanum species: a systematic review. Biomed Res Int. 2013;2013:343808. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5880 mL	12.9400 mL	25.8799 mL	64.6998 mL
	5 mM	0.5176 mL	2.5880 mL	5.1760 mL	12.9400 mL
	10 mM	0.2588 mL	1.2940 mL	2.5880 mL	6.4700 mL
	15 mM	0.1725 mL	0.8627 mL	1.7253 mL	4.3133 mL
	20 mM	0.1294 mL	0.6470 mL	1.2940 mL	3.2350 mL
	25 mM	0.1035 mL	0.5176 mL	1.0352 mL	2.5880 mL
	30 mM	0.0863 mL	0.4313 mL	0.8627 mL	2.1567 mL
	40 mM	0.0647 mL	0.3235 mL	0.6470 mL	1.6175 mL
	50 mM	0.0518 mL	0.2588 mL	0.5176 mL	1.2940 mL
	60 mM	0.0431 mL	0.2157 mL	0.4313 mL	1.0783 mL
	80 mM	0.0323 mL	0.1617 mL	0.3235 mL	0.8087 mL

Praeruptorin A

3 Cited Publications

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Praeruptorin A

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Publications Citing Use of MedChemExpress (MCE) Praeruptorin A

All Calcium Channel Isoforms

All Cytochrome P450 Isoforms

All iGluR Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Praeruptorin A Related Classifications

HY-N6065 Related Classifications