Rezetecan-Cys
Based on 1 Customer Validation
Rezetecan-Cys (SHR9265-Cys) is a Cys-modified form of Rezetecan (HY-148668). Rezetecan is a topoisomerase I inhibitor with cytotoxicity. Rezetecan can be used as a toxic payload for antibody-drug conjugates, such as in the synthesis of Tizetatug rezetecan (HY-177711). Rezetecan can be used in research related to various cancers, including breast cancer and gastric cancer.
For research use only. We do not sell to patients.
- Purity : 98.90%
- CAS No.: 3132870-44-8
- Formula: C58H67FN10O15S
- Molecular Weight:1195.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Topoisomerase Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| LNCaP C4-2 | IC50 |
3.71 nM
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Antiproliferative activity against human C4-2 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Antiproliferative activity against human C4-2 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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41929251 |
| DU-145 | IC50 |
4.29 nM
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Antiproliferative activity against human DU145 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Antiproliferative activity against human DU145 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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41929251 |
| PC-3 | IC50 |
7.74 nM
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Antiproliferative activity against human PC3 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Antiproliferative activity against human PC3 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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41929251 |
| CWR22R | IC50 |
3.04 nM
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Antiproliferative activity against human 22RV1 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Antiproliferative activity against human 22RV1 prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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41929251 |
| LNCaP | IC50 |
2.41 nM
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Antiproliferative activity against human LNCaP prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Antiproliferative activity against human LNCaP prostate cancer cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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41929251 |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3132870-44-8
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Appearance Solid
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Molecular Weight 1195.27
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Formula C58H67FN10O15S
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Color White to off-white
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SMILES
O=C(O)[C@@H](N)CSC(C1)C(N(CCCCCC(NCC(NCC(N[C@@H](CC2=CC=CC=C2)C(NCC(NCO[C@H](C3CC3)C(N[C@H]4CCC5=C6C4=C(CN7C(C(COC([C@@]8(CC)O)=O)=C8C=C79)=O)C9=NC6=CC(F)=C5C)=O)=O)=O)=O)=O)=O)C1=O)=O
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Synonyms
SHR9265-Cys
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (83.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (290 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.8366 mL | 4.1832 mL | 8.3663 mL | 20.9158 mL |
| 5 mM | 0.1673 mL | 0.8366 mL | 1.6733 mL | 4.1832 mL | |
| 10 mM | 0.0837 mL | 0.4183 mL | 0.8366 mL | 2.0916 mL | |
| 15 mM | 0.0558 mL | 0.2789 mL | 0.5578 mL | 1.3944 mL | |
| 20 mM | 0.0418 mL | 0.2092 mL | 0.4183 mL | 1.0458 mL | |
| 25 mM | 0.0335 mL | 0.1673 mL | 0.3347 mL | 0.8366 mL | |
| 30 mM | 0.0279 mL | 0.1394 mL | 0.2789 mL | 0.6972 mL | |
| 40 mM | 0.0209 mL | 0.1046 mL | 0.2092 mL | 0.5229 mL | |
| 50 mM | 0.0167 mL | 0.0837 mL | 0.1673 mL | 0.4183 mL | |
| 60 mM | 0.0139 mL | 0.0697 mL | 0.1394 mL | 0.3486 mL | |
| 80 mM | 0.0105 mL | 0.0523 mL | 0.1046 mL | 0.2614 mL |
Rezetecan-Cys Related Classifications
HY-147408 Related Classifications
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