Rhamnetin
Based on 2 publication(s) in Google Scholar
Rhamnetin is a quercetin derivative found in Coriandrum sativum, inhibits secretory phospholipase A2 and histone deacetylase 2 (HDAC2). Rhamnetin exhibits antitumor, antioxidant and anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity : 99.18%
- CAS No.: 90-19-7
- Formula: C16H12O7
- Molecular Weight:316.27
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Rhamnetin
MoreAll Phospholipase Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HeLa | IC50 |
7.8 μM
Compound: 3
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Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
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[PMID: 19278239] |
| Monocyte | IC50 |
22 μM
Compound: Rhamnetin
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Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA
Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA
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[PMID: 10096854] |
In Vitro
Rhamnetin (0-10 μM, 24-48 h) inhibits the proliferation of cancer cell SKOV3 with an an IC50 of 1.22 μM (24 h)[2].
Rhamnetin (0-10 μM, 24 h) arrests the cell cycle of SKOV3 at G1 phase, and induces apoptosis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat traumatic brain injury model[3]
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Dosage:50-200 mg/kg
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Administration:ip, once daily for 21 days
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Result:Improved the spatial learning and memory ability of rats.
Reduced the levels of TNF-α, IL-1β, IL-6, IL-8, hs-CRP, MDA and H2O2 in the hippocampus.
Increased the levels of IL-10, SOD, GSH-Px in the hippocampus.
Chemical Information
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CAS No. 90-19-7
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Appearance Solid
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Molecular Weight 316.27
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Formula C16H12O7
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Color White to yellow
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SMILES
OC1=C(C2=CC=C(O)C(O)=C2)OC3=C(C(O)=CC(OC)=C3)C1=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (632.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cotton Pellet Granuloma
Cotton pellet granuloma is a classical in vivo chronic inflammation model used to evaluate the anti-inflammatory potential of test substances by measuring their ability to inhibit granuloma tissue formation around an implanted foreign body (cotton pellet) in rodents. The method is based on the biological response to a sterile implanted material, which induces proliferative phase inflammation characterized by fibroblast proliferation and collagen-rich granuloma formation, and the final readout reflects the extent of chronic inflammatory tissue growth surrounding the pellet. In multiple preclinical pharmacological evaluations, inhibition of cotton pellet-induced granuloma formation has been used as an indicator of anti-inflammatory activity in both synthetic and natural product screening contexts.
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Carrageenan-Induced Paw Edema
Carrageenan-induced paw edema is an acute inflammation model in which intraplantar injection of carrageenan induces localized inflammatory swelling characterized by vascular permeability, leukocyte infiltration, and production of inflammatory mediators such as prostaglandins and cytokines, making it widely used to evaluate anti-inflammatory agents in vivo. The resulting paw volume or thickness increase is quantified over time as a direct readout of inflammatory intensity and drug efficacy, typically reflecting cyclooxygenase-mediated prostaglandin-driven edema formation and immune cell recruitment in peripheral tissue[20].
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (276 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Novo Belchor M, et al. Evaluation of Rhamnetin as an Inhibitor of the Pharmacological Effect of Secretory Phospholipase A2. Molecules. 2017 Aug 31;22(9). [Content Brief]
[2]. Bharadwaj KK, et al., Rhamnetin, a nutraceutical flavonoid arrests cell cycle progression of human ovarian cancer (SKOV3) cells by inhibiting the histone deacetylase 2 protein. J Biomol Struct Dyn. 2024;42(24):13421-13436. [Content Brief]
[3]. Zhang W, et al., Rhamnetin attenuates cognitive deficit and inhibits hippocampal inflammatory response and oxidative stress in rats with traumatic brain injury. Cent Eur J Immunol. 2015;40(1):35-41. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1619 mL | 15.8093 mL | 31.6186 mL | 79.0464 mL |
| 5 mM | 0.6324 mL | 3.1619 mL | 6.3237 mL | 15.8093 mL | |
| 10 mM | 0.3162 mL | 1.5809 mL | 3.1619 mL | 7.9046 mL | |
| 15 mM | 0.2108 mL | 1.0540 mL | 2.1079 mL | 5.2698 mL | |
| 20 mM | 0.1581 mL | 0.7905 mL | 1.5809 mL | 3.9523 mL | |
| 25 mM | 0.1265 mL | 0.6324 mL | 1.2647 mL | 3.1619 mL | |
| 30 mM | 0.1054 mL | 0.5270 mL | 1.0540 mL | 2.6349 mL | |
| 40 mM | 0.0790 mL | 0.3952 mL | 0.7905 mL | 1.9762 mL | |
| 50 mM | 0.0632 mL | 0.3162 mL | 0.6324 mL | 1.5809 mL | |
| 60 mM | 0.0527 mL | 0.2635 mL | 0.5270 mL | 1.3174 mL | |
| 80 mM | 0.0395 mL | 0.1976 mL | 0.3952 mL | 0.9881 mL | |
| 100 mM | 0.0316 mL | 0.1581 mL | 0.3162 mL | 0.7905 mL |
Rhamnetin Related Classifications
HY-N7036 Related Classifications
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