2. Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR Epigenetics Apoptosis
  3. FLT3 AMPK Apoptosis
  4. Wu-5

Wu-5 is a USP10 inhibitor that can inhibit FLT3 and AMPK pathways, induce FLT3-ITD degradation and induce apoptosis.

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Wu-5 Chemical Structure

Wu-5 Chemical Structure

CAS No. : 2630378-05-9

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Description

Wu-5 is a USP10 inhibitor that can inhibit FLT3 and AMPK pathways, induce FLT3-ITD degradation and induce apoptosis[1].

In Vitro

Wu-5 (10 μM; 24, 48, 72 h) selectively induces the death of FLT3-ITD-positive AML cells[1].
Wu-5 (1, 2.5, 5 μM; 24, 48 h) induces apoptosis of FLT3-ITD-positive AML cells in a concentration and time-dependent manner[1].
Wu-5 (5 μM; 24 h) induces FLT3-ITD degradation by the proteasome pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Wu-5 Related Antibodies

Cell Viability Assay[1]

Cell Line: U937, HL60, MV4-11, Molm13, and MV4-11-R cells
Concentration: 10 μM
Incubation Time: 24, 48, 72 h
Result: Significantly inhibited the growth of the FLT3-ITD-positive cells (MV4-11, MV4-11-R and Molm13 cells), but had no or little effect on the proliferation of the FLT3-ITD negative cells (U937 and HL60 cells).

Apoptosis Analysis[1]

Cell Line: MV4-11, and Molm13 cells
Concentration: 1, 2.5, 5 μM
Incubation Time: 24, 48 h
Result: Induced the apoptosis of MV4-11 and Molm13 in a concentration and time-dependent manner.

Western Blot Analysis[1]

Cell Line: MV4-11, MV4-11-R, and Molm13 cells
Concentration: 5 μM
Incubation Time: 24 h
Result: Induced FLT3-ITD degradation.
Molecular Weight

351.33

Formula

C15H13NO7S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C(C=C1)OC2=C([N+]([O-])=O)C=C(C(OCC)=O)S2)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (71.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8463 mL 14.2316 mL 28.4633 mL
5 mM 0.5693 mL 2.8463 mL 5.6927 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.34%

COA (254 KB) HNMR (254 KB) LCMS (97 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8463 mL 14.2316 mL 28.4633 mL 71.1582 mL
5 mM 0.5693 mL 2.8463 mL 5.6927 mL 14.2316 mL
10 mM 0.2846 mL 1.4232 mL 2.8463 mL 7.1158 mL
15 mM 0.1898 mL 0.9488 mL 1.8976 mL 4.7439 mL
20 mM 0.1423 mL 0.7116 mL 1.4232 mL 3.5579 mL
25 mM 0.1139 mL 0.5693 mL 1.1385 mL 2.8463 mL
30 mM 0.0949 mL 0.4744 mL 0.9488 mL 2.3719 mL
40 mM 0.0712 mL 0.3558 mL 0.7116 mL 1.7790 mL
50 mM 0.0569 mL 0.2846 mL 0.5693 mL 1.4232 mL
60 mM 0.0474 mL 0.2372 mL 0.4744 mL 1.1860 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Wu-52630378-05-9Wu5Wu 5FLT3AMPKApoptosisCluster of differentiation antigen 135CD135Fms like tyrosine kinase 3AMP-activated protein kinaseU937HL60MV4-11Molm13MV4-11-RAMLCancerInhibitorinhibitorinhibit

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