XD14
Based on 1 Customer Validation
XD14 is a potent BET inhibitor with antitumor effect. It binds to BRD2, BRD3, and BRD4 with Kds of 170, 380, and 160 nM, respectively.
For research use only. We do not sell to patients.
- Purity: 95.02%
- CAS No.: 1370888-71-3
- Formula: C20H27N3O5S
- Molecular Weight:421.51
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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BRD2 170 nM (Kd) |
BRD3 380 nM (Kd) |
BRD4 160 nM (Kd) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | GI50 |
17 μM
Compound: 2; XD14
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Growth inhibition of human HL60 cells after 72 hrs by CellTiter 96S AQeous non-radioactive cell proliferation assay
Growth inhibition of human HL60 cells after 72 hrs by CellTiter 96S AQeous non-radioactive cell proliferation assay
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[PMID: 33275431] |
Chemical Information
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CAS No. 1370888-71-3
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Appearance Solid
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Molecular Weight 421.51
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Formula C20H27N3O5S
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Color Off-white to light yellow
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SMILES
O=C(C1=C(CC)C(C(C)=O)=C(C)N1)NC2=CC(S(=O)(N(CC)CC)=O)=CC=C2O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (296.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3724 mL | 11.8621 mL | 23.7242 mL | 59.3106 mL |
| 5 mM | 0.4745 mL | 2.3724 mL | 4.7448 mL | 11.8621 mL | |
| 10 mM | 0.2372 mL | 1.1862 mL | 2.3724 mL | 5.9311 mL | |
| 15 mM | 0.1582 mL | 0.7908 mL | 1.5816 mL | 3.9540 mL | |
| 20 mM | 0.1186 mL | 0.5931 mL | 1.1862 mL | 2.9655 mL | |
| 25 mM | 0.0949 mL | 0.4745 mL | 0.9490 mL | 2.3724 mL | |
| 30 mM | 0.0791 mL | 0.3954 mL | 0.7908 mL | 1.9770 mL | |
| 40 mM | 0.0593 mL | 0.2966 mL | 0.5931 mL | 1.4828 mL | |
| 50 mM | 0.0474 mL | 0.2372 mL | 0.4745 mL | 1.1862 mL | |
| 60 mM | 0.0395 mL | 0.1977 mL | 0.3954 mL | 0.9885 mL | |
| 80 mM | 0.0297 mL | 0.1483 mL | 0.2966 mL | 0.7414 mL | |
| 100 mM | 0.0237 mL | 0.1186 mL | 0.2372 mL | 0.5931 mL |
XD14 Related Classifications
HY-110215 Related Classifications
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