2. JAK/STAT Signaling Stem Cell/Wnt Protein Tyrosine Kinase/RTK Epigenetics Anti-infection
  3. STAT JAK HSV
  4. 2-NP

2-NP is a selective enhancer of STAT1 transcription. 2-NP can enhance the ability of IFN-γ to inhibit the proliferation of human breast cancer and fibrosarcoma cells.

For research use only. We do not sell to patients.

CAS No. : 65182-56-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 13 publication(s) in Google Scholar

2-NP Related Antibodies

Top Publications Citing Use of Products

    2-NP purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e04569.  [Abstract]

    2-NP (45 × 10−6 M; 2 h) induced enhancement of STAT1 phosphorylation in PBMC-derived macrophages.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) aggravated the disordered hepatocytes and inflammatory cell infiltration in CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) diminished therapeutic effect of COP on inflammatory factors evidenced by the detection of IL-6 and TNF-α in the serums of CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) diminished the effect of COP on reducing MDA and increasing GSH in liver tissues in the serums of CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) weakened therapeutic effect of COP on restoring GPX4 and inhibiting the phosphorylation of STAT1 and IRF1 in CLP (cecal ligation and puncture) mice.

    View All STAT Isoform Specific Products:

    View All Isoforms
    STAT3 STAT5 STAT6 STAT1

    View All JAK Isoform Specific Products:

    View All Isoforms
    JAK1 JAK2 JAK3 Tyk2 JAK

    View All HSV Isoform Specific Products:

    View All Isoforms
    HSV-1 HSV-2 HSV

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    2-NP is a selective enhancer of STAT1 transcription. 2-NP can enhance the ability of IFN-γ to inhibit the proliferation of human breast cancer and fibrosarcoma cells[1].

    In Vitro

    2-NP (5 μmol/mL, 24 h) inhibits the viral replication of Duck plague virus (DPV) through activation of the JAK-STAT signaling pathway in duck embryo fibroblasts (DEF)[2].
    2-NP (45 μM, 48 h) promotes the expression of CXCR3 in mouse plasmacytoid dendritic cells (pDCs), promotes CXCL10-induced pDC migration[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2-NP Related Antibodies

    Western Blot Analysis[3]

    Cell Line: mouse plasmacytoid dendritic cell
    Concentration: 45 μM
    Incubation Time: 48 h
    Result: Increased the phosphorylation of JAK1 and STAT1.

    Cell Migration Assay [3]

    Cell Line: mouse plasmacytoid dendritic cell
    Concentration: 45 μM
    Incubation Time: 48 h
    Result: Promoted the migration of pDCs.
    In Vivo

    2-NP (0.5 mg/kg, subconjunctival injection, once daily for 3 days) alleviates corneal lesions and exhibits antiviral activity in Herpes simplex keratitis (HSK) mouse model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Herpes simplex keratitis (HSK) model[3]
    Dosage: 0.5 mg/kg
    Administration: subconjunctival injection, once daily for 3 days
    Result: Alleviated corneal lesions in mice, reduced corneal opacity and neovascularization.
    Molecular Weight

    222.25

    Formula

    C14H10N2O
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    OC1=CC=CC=C1C2=NC3=NC=CC=C3C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (224.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4994 mL 22.4972 mL 44.9944 mL
    5 mM 0.8999 mL 4.4994 mL 8.9989 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (5.62 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.4994 mL 22.4972 mL 44.9944 mL 112.4859 mL
    5 mM 0.8999 mL 4.4994 mL 8.9989 mL 22.4972 mL
    10 mM 0.4499 mL 2.2497 mL 4.4994 mL 11.2486 mL
    15 mM 0.3000 mL 1.4998 mL 2.9996 mL 7.4991 mL
    20 mM 0.2250 mL 1.1249 mL 2.2497 mL 5.6243 mL
    25 mM 0.1800 mL 0.8999 mL 1.7998 mL 4.4994 mL
    30 mM 0.1500 mL 0.7499 mL 1.4998 mL 3.7495 mL
    40 mM 0.1125 mL 0.5624 mL 1.1249 mL 2.8121 mL
    50 mM 0.0900 mL 0.4499 mL 0.8999 mL 2.2497 mL
    60 mM 0.0750 mL 0.3750 mL 0.7499 mL 1.8748 mL
    80 mM 0.0562 mL 0.2812 mL 0.5624 mL 1.4061 mL
    100 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1249 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    2-NP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2-NP65182-56-1STATJAKHSVJanus kinaseHerpes simplex virusbreast cancerfibrosarcomaC57BL/6J mouseInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    2-NP
    Cat. No.:
    HY-W013523
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (614 KB) Français - FR (614 KB) Deutsch - DE (614 KB) Norwegian - NO (614 KB) Español - ES (614 KB) Swedish - SV (614 KB) Italian - IT (614 KB) Korean - KR (614 KB) Portuguese - PT (614 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.