653-47 hydrochloride
Based on 1 Customer Validation
653-47 hydrochloride, a potentiator, significantly potentiates the cAMP-response element binding protein (CREB) inhibitory activity of 666-15. 653-47 hydrochloride is also a very weak CREB inhibitor with IC50 of 26.3 μM.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.42%
- CAS. Nr.: 1224567-46-7
- Formel: C20H20Cl2N2O3
- Molecular Weight:407.29
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
2.73 μM
Compound: 14
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Growth inhibition of human A549 cells after 72 hrs by MTT assay
Growth inhibition of human A549 cells after 72 hrs by MTT assay
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[PMID: 25313320] |
| HEK-293T | IC50 |
26.25 μM
Compound: 14
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Inhibition of CREB (unknown origin) expressed in HEK293T cells assessed as reduction in CREB-mediated gene tanscription after 5 hrs by luciferase reporter gene assay
Inhibition of CREB (unknown origin) expressed in HEK293T cells assessed as reduction in CREB-mediated gene tanscription after 5 hrs by luciferase reporter gene assay
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[PMID: 25313320] |
| MCF7 | GI50 |
1.85 μM
Compound: 14
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Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
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[PMID: 25313320] |
| MDA-MB-231 | GI50 |
2.19 μM
Compound: 14
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Growth inhibition of human MDA-MB-231 cells after 72 hrs by MTT assay
Growth inhibition of human MDA-MB-231 cells after 72 hrs by MTT assay
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[PMID: 25313320] |
| MDA-MB-468 | GI50 |
1.2 μM
Compound: 14
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Growth inhibition of human MDA-MB-468 cells after 72 hrs by MTT assay
Growth inhibition of human MDA-MB-468 cells after 72 hrs by MTT assay
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[PMID: 25313320] |
In Vitro
653-47 (5-10 μM) synergistically inhibits CREB-mediated gene transcription with 666-15[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1224567-46-7
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Appearance Solid
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Molecular Weight 407.29
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Formel C20H20Cl2N2O3
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Color Pale purple to purple
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SMILES
O=C(C1=C(OCCCN)C=C2C=CC=CC2=C1)NC3=CC=C(Cl)C=C3O.[H]Cl
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 250 mg/mL (613.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4553 mL | 12.2763 mL | 24.5525 mL | 61.3813 mL |
| 5 mM | 0.4911 mL | 2.4553 mL | 4.9105 mL | 12.2763 mL | |
| 10 mM | 0.2455 mL | 1.2276 mL | 2.4553 mL | 6.1381 mL | |
| 15 mM | 0.1637 mL | 0.8184 mL | 1.6368 mL | 4.0921 mL | |
| 20 mM | 0.1228 mL | 0.6138 mL | 1.2276 mL | 3.0691 mL | |
| 25 mM | 0.0982 mL | 0.4911 mL | 0.9821 mL | 2.4553 mL | |
| 30 mM | 0.0818 mL | 0.4092 mL | 0.8184 mL | 2.0460 mL | |
| 40 mM | 0.0614 mL | 0.3069 mL | 0.6138 mL | 1.5345 mL | |
| 50 mM | 0.0491 mL | 0.2455 mL | 0.4911 mL | 1.2276 mL | |
| 60 mM | 0.0409 mL | 0.2046 mL | 0.4092 mL | 1.0230 mL | |
| 80 mM | 0.0307 mL | 0.1535 mL | 0.3069 mL | 0.7673 mL | |
| 100 mM | 0.0246 mL | 0.1228 mL | 0.2455 mL | 0.6138 mL |
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