653-47 hydrochloride

Name: 653-47 hydrochloride
Brand: MedChemExpress
SKU: HY-134598A
Rating: 4.5 (1 reviews)

Cat. No.: HY-134598A Purity: 99.42%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

653-47 hydrochloride, a potentiator, significantly potentiates the cAMP-response element binding protein (CREB) inhibitory activity of 666-15. 653-47 hydrochloride is also a very weak CREB inhibitor with IC₅₀ of 26.3 μM.

For research use only. We do not sell to patients.

653-47 hydrochloride Chemical Structure

CAS No. : 1224567-46-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 275	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 275	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 653-47 hydrochloride:

653-47 Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

653-47 (5-10 μM) synergistically inhibits CREB-mediated gene transcription with 666-15^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

407.29

Formula

C₂₀H₂₀Cl₂N₂O₃

CAS No.

1224567-46-7

Appearance

Solid

Color

Pale purple to purple

SMILES

O=C(C1=C(OCCCN)C=C2C=CC=CC2=C1)NC3=CC=C(Cl)C=C3O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (613.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4553 mL	12.2763 mL	24.5525 mL
5 mM	0.4911 mL	2.4553 mL	4.9105 mL
10 mM	0.2455 mL	1.2276 mL	2.4553 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Xie F, et al. Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666-15. J Med Chem. 2019;62(24):11423-11429. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4553 mL	12.2763 mL	24.5525 mL	61.3813 mL
	5 mM	0.4911 mL	2.4553 mL	4.9105 mL	12.2763 mL
	10 mM	0.2455 mL	1.2276 mL	2.4553 mL	6.1381 mL
	15 mM	0.1637 mL	0.8184 mL	1.6368 mL	4.0921 mL
	20 mM	0.1228 mL	0.6138 mL	1.2276 mL	3.0691 mL
	25 mM	0.0982 mL	0.4911 mL	0.9821 mL	2.4553 mL
	30 mM	0.0818 mL	0.4092 mL	0.8184 mL	2.0460 mL
	40 mM	0.0614 mL	0.3069 mL	0.6138 mL	1.5345 mL
	50 mM	0.0491 mL	0.2455 mL	0.4911 mL	1.2276 mL
	60 mM	0.0409 mL	0.2046 mL	0.4092 mL	1.0230 mL
	80 mM	0.0307 mL	0.1535 mL	0.3069 mL	0.7673 mL
	100 mM	0.0246 mL	0.1228 mL	0.2455 mL	0.6138 mL