Integrin antagonist 27
Based on 1 Customer Validation
Integrin antagonist 27 (Compound 27) is a small molecule integrin αvβ3 antagonist with an affinity of 18 nM. Integrin antagonist 27 can be combined with Paclitaxel (HY-B0015) to selectively target αvβ3-positive metastatic cancer cells. Integrin antagonist 27 is used as an anticancer agent.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 593274-97-6
- Formula: C24H20N4O5
- Molecular Weight:444.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEY | IC50 |
>20 μM
Compound: 27
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Cytotoxicity against human HEY cell line by MTT assay
Cytotoxicity against human HEY cell line by MTT assay
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[PMID: 16854058] |
| MCF7 | IC50 |
>20 μM
Compound: 27
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Cytotoxicity against human MCF7 cell line by MTT assay
Cytotoxicity against human MCF7 cell line by MTT assay
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[PMID: 16854058] |
| MDA-MB-435 | IC50 |
>20 μM
Compound: 27
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Cytotoxicity against human MDA-MB-435 cell line by MTT assay
Cytotoxicity against human MDA-MB-435 cell line by MTT assay
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[PMID: 16854058] |
| NCI-H1975 | IC50 |
>20 μM
Compound: 27
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Cytotoxicity against human NCI-H1975 cell line by MTT assay
Cytotoxicity against human NCI-H1975 cell line by MTT assay
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[PMID: 16854058] |
| NIH3T3 | IC50 |
>20 μM
Compound: 27
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Cytotoxicity against mouse NIH3T3 cell line by MTT assay
Cytotoxicity against mouse NIH3T3 cell line by MTT assay
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[PMID: 16854058] |
Integrin antagonist 27 can be combined with Paclitaxel (HY-B0015) to selectively target αvβ3-positive metastatic cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 593274-97-6
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Appearance Solid
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Molecular Weight 444.44
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Formula C24H20N4O5
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Color Light yellow to yellow
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SMILES
O=C(O)C1=CC=CC(N2C(/C=C(C(N3CC(NC4=CC=C(C)C=C4)=O)=O)\NC3=O)=CC=C2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 100 mg/mL (225.00 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.63 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2500 mL | 11.2501 mL | 22.5002 mL | 56.2506 mL |
| 5 mM | 0.4500 mL | 2.2500 mL | 4.5000 mL | 11.2501 mL | |
| 10 mM | 0.2250 mL | 1.1250 mL | 2.2500 mL | 5.6251 mL | |
| 15 mM | 0.1500 mL | 0.7500 mL | 1.5000 mL | 3.7500 mL | |
| 20 mM | 0.1125 mL | 0.5625 mL | 1.1250 mL | 2.8125 mL | |
| 25 mM | 0.0900 mL | 0.4500 mL | 0.9000 mL | 2.2500 mL | |
| 30 mM | 0.0750 mL | 0.3750 mL | 0.7500 mL | 1.8750 mL | |
| 40 mM | 0.0563 mL | 0.2813 mL | 0.5625 mL | 1.4063 mL | |
| 50 mM | 0.0450 mL | 0.2250 mL | 0.4500 mL | 1.1250 mL | |
| 60 mM | 0.0375 mL | 0.1875 mL | 0.3750 mL | 0.9375 mL | |
| 80 mM | 0.0281 mL | 0.1406 mL | 0.2813 mL | 0.7031 mL | |
| 100 mM | 0.0225 mL | 0.1125 mL | 0.2250 mL | 0.5625 mL |
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