2. PI3K/Akt/mTOR Metabolic Enzyme/Protease PROTAC
  3. mTOR Endogenous Metabolite Ligands for E3 Ligase
  4. L-Leucine

L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway.

For research use only. We do not sell to patients.

CAS No. : 61-90-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Based on 16 publication(s) in Google Scholar

Other Forms of L-Leucine:

L-Leucine Related Antibodies

Top Publications Citing Use of Products

    L-Leucine purchased from MedChemExpress. Usage Cited in: Acta Neuropathol Commun. 2025 Nov 4;13(1):223.  [Abstract]

    Western blot analysis of p-mTOR, total mTOR, p-TFEB, total TFEB, and β-actin in shNT or shTAL-transfected MN9D cells treated with either PBS or L-Leucine (100 μM; 36 h), a mTORC1 activator.

    L-Leucine purchased from MedChemExpress. Usage Cited in: Acta Neuropathol Commun. 2025 Nov 4;13(1):223.  [Abstract]

    Quantitative analysis of p-mTOR/mTOR and p-TFEB/TFEB ratios demonstrates that TAL1 knockdown decreases mTORC1 activity and TFEB phosphorylation, and this effect is attenuated by L-Leucine (100 μM; 36 h) treatment.

    L-Leucine purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 Jan-Dec:21:17448069251376198.  [Abstract]

    Injection of mTOR agonist L-leucine induced an upregulation of the protein expression of BDNF in the spinal cord of mice. (Adult male CD-1 mice, KU-0063794 was administered intraperitoneally (i.p.) at 8 mg/kg, L-leucine was administered i.p. at 50 mg/kg).

    L-Leucine purchased from MedChemExpress. Usage Cited in: Mol Pain. 2025 Jan-Dec:21:17448069251376198.  [Abstract]

    Pharmacological S6K1 inhibition by PF-4708671 significantly reversed L-leucine-induced thermal hyperalgesia. (Adult male CD-1 mice, 50 mM PF-4708671 (0.5 mL) was injected i.p. at 40 mg/kg per mouse, L-leucine was administered i.p. at 50 mg/kg).

    L-Leucine purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2023 Mar:361:114315.  [Abstract]

    Representative immunostaining results of mcherry (in Red), GFAP (in Green). Spinal cord was treated with Ctrl or Fign shRNA. Propriospinal neurons were label with mcherry. 1 day after SCI (spinal cord injury), L- leucine (10 mg/mL) or saline was administered by tail vein injection at a dose of 1 mg/30 g body weight every other day for 28 days.

    View All mTOR Isoform Specific Products:

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    mTOR mTORC1 mTORC2

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    Human Endogenous Metabolite Microbial Metabolite

    View All Ligands for E3 Ligase Isoform Specific Products:

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    von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1 TRIM21 NEURL1B KLHL41

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1].

    IC50 & Target[1][2]

    mTORC1

     

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    In Vitro

    L-Leucine (10 mM) treatment impairs endocrine progenitor cell development[1].
    In E13.5 rat pancreatic explants, in absence of added L-Leucine, Ngn3 mRNA levels increased after 1 day of culture, peaked on day 3, and then decreased. When L-Leucine is added, a dramatic decrease is observed in Ngn3 mRNA levels. This decrease in Ngn3 mRNA levels was paralleled by a decrease in the number of Ngn3-expressing cells (4728±408 vs. 959±28; P<0.01). Finally, L-Leucine also caused a dose-dependent repressive effect on the mRNA levels of the three genes, namely Ngn3, its target Insm1, and insulin[1].
    Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. L-Leucine increases intracellular HIF-1α levels and activates the HIF-1α signaling pathway, and these two effects are mediated by the mTOR signaling pathway. This process results in Ngn3 repression and, consequently, decreases β-cell differentiation[1].
    L-leucine stimulates mTORC1 by through a mechanism that involves the leucyl tRNA synthase promoting the activity of GTP activating proteins that act on mTORC1[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Leucine Related Antibodies
    In Vivo

    L-Leucine (24 g/kg diet) combined with Resveratrol (12.5 mg/kg diet) to diet-induced obese (DIO) mice increases adipose Sirt1 activity[2].
    Combination results in significant reductions in body weight, weight gain, visceral adipose tissue mass, fat oxidation and heat production in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Six-week-old male c57/BL6 mice (fed a high-fat diet with fat to induce obesity)[1]
    Dosage: 24 g/kg diet; Resveratrol (low dose; 12.5 mg/kg diet)
    Administration: 6 weeks
    Result: Treatment in combination with Resveratrol (low dose; 12.5 mg/kg diet) resulted in significant reductions in body weight, weight gain, visceral adipose tissue mass, fat oxidation and heat production, and an associated decrease in respiratory exchange ratio (RER), especially in the dark (feeding) cycle.
    Clinical Trial
    Molecular Weight

    131.17

    Formula

    C6H13NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CC(C)C)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 8.33 mg/mL (63.51 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.6237 mL 38.1185 mL 76.2369 mL
    5 mM 1.5247 mL 7.6237 mL 15.2474 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 6.25 mg/mL (47.65 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.79%

    SDS (396 KB)

    COA (245 KB) HNMR (147 KB) RP-HPLC (102 KB) OR (364 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 7.6237 mL 38.1185 mL 76.2369 mL 190.5924 mL
    5 mM 1.5247 mL 7.6237 mL 15.2474 mL 38.1185 mL
    10 mM 0.7624 mL 3.8118 mL 7.6237 mL 19.0592 mL
    15 mM 0.5082 mL 2.5412 mL 5.0825 mL 12.7062 mL
    20 mM 0.3812 mL 1.9059 mL 3.8118 mL 9.5296 mL
    25 mM 0.3049 mL 1.5247 mL 3.0495 mL 7.6237 mL
    30 mM 0.2541 mL 1.2706 mL 2.5412 mL 6.3531 mL
    40 mM 0.1906 mL 0.9530 mL 1.9059 mL 4.7648 mL
    50 mM 0.1525 mL 0.7624 mL 1.5247 mL 3.8118 mL
    60 mM 0.1271 mL 0.6353 mL 1.2706 mL 3.1765 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Leucine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Leucine61-90-5LeucinemTOREndogenous MetaboliteLigands for E3 LigaseMammalian target of RapamycinE3 ligase-recruiting MoietyInhibitorinhibitorinhibit

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