1. PI3K/Akt/mTOR
  2. mTOR
  3. L-Leucine

L-Leucine 

Cat. No.: HY-N0486 Purity: >98.0%
Handling Instructions

L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway.

For research use only. We do not sell to patients.

L-Leucine Chemical Structure

L-Leucine Chemical Structure

CAS No. : 61-90-5

Size Price Stock Quantity
10 mM * 1  mL in Water USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

View All mTOR Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway[1].

IC50 & Target[1]

mTORC1

 

In Vitro

L-Leucine (10 mM) treatment impairs endocrine progenitor cell development[1].
In E13.5 rat pancreatic explants, in absence of added L-Leucine, Ngn3 mRNA levels increased after 1 day of culture, peaked on day 3, and then decreased. When L-Leucine is added, a dramatic decrease is observed in Ngn3 mRNA levels. This decrease in Ngn3 mRNA levels was paralleled by a decrease in the number of Ngn3-expressing cells (4728±408 vs. 959±28; P<0.01). Finally, L-Leucine also caused a dose-dependent repressive effect on the mRNA levels of the three genes, namely Ngn3, its target Insm1, and insulin[1].
Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. L-Leucine increases intracellular HIF-1α levels and activates the HIF-1α signaling pathway, and these two effects are mediated by the mTOR signaling pathway. This process results in Ngn3 repression and, consequently, decreases β-cell differentiation[1].

In Vivo

L-Leucine (24 g/kg diet) combined with Resveratrol (12.5 mg/kg diet) to diet-induced obese (DIO) mice increases adipose Sirt1 activity[2].
Combination results in significant reductions in body weight, weight gain, visceral adipose tissue mass, fat oxidation and heat production in mice[2].

Animal Model: Six-week-old male c57/BL6 mice (fed a high-fat diet with fat to induce obesity)[1]
Dosage: 24 g/kg diet; Resveratrol (low dose; 12.5 mg/kg diet)
Administration: 6 weeks
Result: Treatment in combination with Resveratrol (low dose; 12.5 mg/kg diet) resulted in significant reductions in body weight, weight gain, visceral adipose tissue mass, fat oxidation and heat production, and an associated decrease in respiratory exchange ratio (RER), especially in the dark (feeding) cycle.
Clinical Trial
Molecular Weight

131.17

Formula

C₆H₁₃NO₂

CAS No.

61-90-5

SMILES

N[[email protected]@H](CC(C)C)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 8.33 mg/mL (63.51 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.6237 mL 38.1185 mL 76.2369 mL
5 mM 1.5247 mL 7.6237 mL 15.2474 mL
10 mM 0.7624 mL 3.8118 mL 7.6237 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: >98.0%

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

L-LeucinemTORMammalian target of RapamycinInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product name:
L-Leucine
Cat. No.:
HY-N0486
Quantity:
MCE Japan Authorized Agent: