2. Anti-infection Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Antibiotic PDI
  4. Ribostamycin sulfate

Ribostamycin sulfate  (Synonyms: Vistamycin sulfate)

Cat. No.: HY-B1228 Purity: 99.55%
COA
SDS
Handling Instructions Technical Support

Ribostamycin sulfate (Vistamycin sulfate) is a broad-spectrum aminoglycoside Antibiotic with bactericidal activity against Gram-positive cocci, Gram-negative cocci, bacilli, and drug-resistant strains. Ribostamycin sulfate also acts as an inhibitor of protein disulfide isomerase (PDI), with a binding constant KD of 319 μM for bovine PDI. Ribostamycin sulfate targets bacterial 16S ribosomal RNA and the 30S ribosomal subunit, causing translational misreading and thereby inhibiting bacterial protein synthesis. Ribostamycin sulfate disrupts the integrity of bacterial cell membranes, induces membrane pore formation, and leads to bacterial death. Ribostamycin sulfate can be used in studies related to bacterial infections.

For research use only. We do not sell to patients.

Ribostamycin sulfate

Ribostamycin sulfate Chemical Structure

CAS No. : 53797-35-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of Ribostamycin sulfate:

Ribostamycin sulfate Related Antibodies

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Description

Ribostamycin sulfate (Vistamycin sulfate) is a broad-spectrum aminoglycoside Antibiotic with bactericidal activity against Gram-positive cocci, Gram-negative cocci, bacilli, and drug-resistant strains. Ribostamycin sulfate also acts as an inhibitor of protein disulfide isomerase (PDI), with a binding constant KD of 319 μM for bovine PDI. Ribostamycin sulfate targets bacterial 16S ribosomal RNA and the 30S ribosomal subunit, causing translational misreading and thereby inhibiting bacterial protein synthesis. Ribostamycin sulfate disrupts the integrity of bacterial cell membranes, induces membrane pore formation, and leads to bacterial death. Ribostamycin sulfate can be used in studies related to bacterial infections[1][2][3][4].
IC50 & Target[4]

Aminoglycoside

 

In Vitro

Ribostamycin sulfate can bind to purified bovine protein disulfide isomerase (PDI) with a dissociation constant of 319 μM; it can inhibit the chaperone activity of purified bovine PDI, but does not inhibit the autodegradation activity of purified bovine PDI[1].
Ribostamycin (0.25-64 μg/mL; 14 h) sulfate potently inhibits Haemophilus influenzae ATCC 49247 (MIC = 0.5 μM), Streptococcus pneumoniae ATCC 49619 (MIC = 7.2 μM), and three Escherichia coli strains (ATCC 4157, ATCC 12435, ATCC 10798; MIC range 0.9-7.2 μM), shows moderate activity against two other E. coli strains, and has very weak or no activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis strains in vitro[2].
Ribostamycin (0.25-64 μg/mL plus 2640 μM EDTA (HY-Y0682); 14 h) sulfate co-treatment with EDTA reduces the MIC of ribostamycin 8-fold against β-lactam-resistant Escherichia coli ATCC 35218, has no effect on its activity against E. coli ATCC 25922 or Pseudomonas aeruginosa ATCC 27853, and reduces its MIC against Staphylococcus aureus ATCC 12600 to <0.1 μM in vitro[2].
Ribostamycin (68 h) sulfate exhibits no cytotoxicity against HEK293 and MDCKII kidney cells[2].
Ribostamycin (72 h for MIC, 72 h exposure followed by 3-week subculture for MBC) sulfate shows no significant in vitro inhibitory or borreliacidal activity against 11 Borrelia burgdorferi sensu lato isolates[3].
Ribostamycin (500 μM-1 mM) sulfate forms linear oligomers that mediate the assembly of citrate-capped AuNPs (40 nm, 60 nm, 80 nm, 100 nm) into visible linear structures, with the most robust assemblies formed with 40 nm and 60 nm AuNPs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ribostamycin sulfate Related Antibodies
Molecular Weight

552.55

Formula

C17H36N4O14S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(O)(O)=O.O[C@@H]([C@H](O)[C@@H](CN)O1)[C@@H](N)[C@@]1([H])O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@@]2([H])O[C@@H]3O[C@H](CO)[C@@H](O)[C@H]3O
Structure Classification
Initial Source

Streptomyces ribosidicus
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 130 mg/mL (235.27 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8098 mL 9.0490 mL 18.0979 mL
5 mM 0.3620 mL 1.8098 mL 3.6196 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (90.49 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.55%

SDS (419 KB)

COA (258 KB) HNMR (301 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.8098 mL 9.0490 mL 18.0979 mL 45.2448 mL
5 mM 0.3620 mL 1.8098 mL 3.6196 mL 9.0490 mL
10 mM 0.1810 mL 0.9049 mL 1.8098 mL 4.5245 mL
15 mM 0.1207 mL 0.6033 mL 1.2065 mL 3.0163 mL
20 mM 0.0905 mL 0.4524 mL 0.9049 mL 2.2622 mL
25 mM 0.0724 mL 0.3620 mL 0.7239 mL 1.8098 mL
30 mM 0.0603 mL 0.3016 mL 0.6033 mL 1.5082 mL
40 mM 0.0452 mL 0.2262 mL 0.4524 mL 1.1311 mL
50 mM 0.0362 mL 0.1810 mL 0.3620 mL 0.9049 mL
60 mM 0.0302 mL 0.1508 mL 0.3016 mL 0.7541 mL
80 mM 0.0226 mL 0.1131 mL 0.2262 mL 0.5656 mL
100 mM 0.0181 mL 0.0905 mL 0.1810 mL 0.4524 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ribostamycin sulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ribostamycin53797-35-6VistamycinAntibioticPDIProtein Disulfide IsomerasesHEK29330S ribosomal subunitProtein Disulfide IsomeraseBorrelia burgdorferiHaemophilus influenzae ATCC 49247kidney cellsbacterial 16S ribosomal RNAEscherichia coliStreptococcus pneumoniae ATCC 49619bovine PDIInhibitorinhibitorinhibit

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