1. Cell Cycle/DNA Damage
    Autophagy
    Anti-infection
  2. DNA/RNA Synthesis
    DNA Alkylator/Crosslinker
    Autophagy
    Bacterial
    Antibiotic
  3. Streptozocin

Streptozocin (Synonyms: Streptozotocin; U 9889)

製品番号: HY-13753 純度: 99.58%
取扱説明書

Streptozocin is a potent DNA-methylating antibiotic. Streptozotocin causes methylation of liver and kidney and pancreatic DNA, but no methylation in brain DNA.

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Streptozocin 構造式

Streptozocin 構造式

CAS 番号 : 18883-66-4

容量 価格(税別) 在庫状況 数量
無料サンプル (0.5-1 mg)   今すぐ申し込む  
100 mg USD 60 在庫あり
Estimated Time of Arrival: December 31
500 mg USD 150 在庫あり
Estimated Time of Arrival: December 31
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 6 publication(s) in Google Scholar

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製品説明

Streptozocin is a potent DNA-methylating antibiotic. Streptozotocin causes methylation of liver and kidney and pancreatic DNA, but no methylation in brain DNA.

IC50 & Target

DNA alkylator[1]

体外実験

Streptozocin (STZ) shows higher cytotoxic effect in vitro on hematological cell lines compared to Alloxan (ALX). ALX appeares not to be toxic for the studied cell lines with estimated IC50 values of 2809, 3679 or over 4000 μg/mL for HL60, K562 and C1498 cells, respectively. Streptozocin is more toxic, especially for the human myeloid leukemia cell line, HL60. The IC50 values of Streptozocin are 11.7, 904 and 1024 μg/mL for HL60, K562 and C1498 cells, respectively. Results also show that the murine leukemic cells are more resistant to Streptozocin and ALX cytotoxicity than human leukemic cells[2].

体内実験

Streptozocin (STZ)-injected mice show tendency to have lower body weight than that observed in animals injected with ALX. Streptozocin -injected mice have significantly fewer splenocytes (22.2±3.2×106; n=10) compared to mice injected with ALX (60.7±4.3×106; n=15; p=0.01)[2].

臨床実験
分子量

265.22

分子式

C₈H₁₅N₃O₇

CAS 番号

18883-66-4

SMILES
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件
Powder -20°C 3 years
  4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

溶剤 & 溶解度
体外: 

DMSO : 130 mg/mL (490.16 mM; Need ultrasonic)

H2O : 113.3 mg/mL (427.19 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7705 mL 18.8523 mL 37.7045 mL
5 mM 0.7541 mL 3.7705 mL 7.5409 mL
10 mM 0.3770 mL 1.8852 mL 3.7705 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (9.73 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.58 mg/mL (9.73 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.58 mg/mL (9.73 mM); Clear solution

*All of the co-solvents are provided by MCE.
参考文献
細胞実験
[2]

Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dH2O in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

動物実験
[2][3]

Mice[2]
Male C57BL/6 mice (10-16 weeks) are used.The age group distribution in the mouse group treated with Streptozocin and ALX as well as controls is as follows: Streptozocin xenograft (n=7, median age 14 weeks), ALX xenograft (n=11, median age 15 weeks), Streptozocin non-transplanted (n=7, median age 14 weeks), ALX non-transplanted (n=15, median age 15 weeks).Male C57BL/6 mice are under inhalation anesthesia injected via the penile vein with ALX (75 mg/mL) or Streptozocin (180 mg/kg). Control group contain male C57BL/6 mice. Blood glucose levels and body weight are measured before injection, after 6 h, then daily after drug injection.
Rats[3]
Thirty rats underwent oophorectomy to induce menopausal status. Rats receive intraperitoneal administration of Streptozocin (50 mg/kg) to induce diabetes mellitus (DM) 1 week after the oophorectomy. Blood glucose level is checked 3 days after Streptozocin administration, and values >250 mg/dL are considered as positive for DM.

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

参考文献

純度: 99.58%

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The molarity calculator equation

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Keywords:

StreptozocinStreptozotocinU 9889U9889U-9889DNA/RNA SynthesisDNA Alkylator/CrosslinkerAutophagyBacterialAntibioticInhibitorinhibitorinhibit

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製品名:
Streptozocin
製品番号:
HY-13753
数量:
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