Art. -Nr.: HY-B1207

Urethane

(Synonyms: Ethyl carbamate; Carbamic acid ethyl ester; Ethylurethane)

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

Urethane (Ethyl carbamate), the ethyl ester of carbamic acid, is a byproduct of fermentation found in various food products. Urethane has the ability to suppress bacterial, protozoal, sea urchin egg, and plant tissue growth in vitro.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.35%
CAS. Nr.: 51-79-6
Formel: C₃H₇NO₂
Molecular Weight:89.09

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Urethane Chemical Structure

CAS. Nr.: 51-79-6

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Induced Disease Models
- Clinical Trial
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Parasite Isoform-spezifische Produkte anzeigen :

In Vitro

Urethane is a good clastogen in mammalian somatic cells in vivo, but it shows variable results with cells in vitro. Urethane efficiently induces sister chromatid exchanges in a variety of cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Urethane can be used in animal modeling to create lung tumor models. Approximately 90% of Urethane is hydrolyzed to ethanol, ammonia, and carbon dioxide by hepatic microsomal esterases (carboxylesterases), while only 5% of Urethane is excreted unchanged. A very small amount of Urethane is converted by the cytochrome P450 enzyme CYP2E1 to ethylene urea, leading to the formation of an epoxide (ethylene urea epoxide). This epoxide covalently binds to DNA and may cause base misincorporation during DNA replication, thereby inducing point mutations^[1]^[2].

Induction of Lung Tumor^[1]^[2]^[3]

Background

Exposure of mouse to Urethane primarily induces tumors in lung with a single driver mutation in Kras gene at position Q61 with one substitution L or R according to mouse strain. The activation of an oncogenic Kras allele in mice was shown to be tumorigenic in multiple organs, such as liver and pancreas^[2].

Specific Modeling Methods

Mice: hPRDX4 transgenic (Tg) and non-Tg C57BL/6• male• 8 weeks old^[1]
Administration: 1 g/kg (in 100 μL saline)• i.p.• once per week for 16 weeks or every other day for a week^[1]

Note

(1) Before administration, all mice were kept in a room with relatively constant temperature (21-23°C) humidity (50-60%), and 12 h light/dark circle. They received a standard chow diet and purified tap water ad libitum^[1].
(2) After mice were sacrificed, lungs were harvested, and the number of tumors and their diameter on the lung surface were measured. Serum was also collected after centrifugation^[1].

Modeling Indicators

Molecular changes: Urethane administration decreased the lavels of proapoptotic genes (BAX, BAD) no significant difference was found in antiapoptotic genes (BCL2, BCL2L1) in Tg tumor. Urethane administration elevated MMP9 and IL-1β expressions in Tg tumor^[1].
Tumor changes: Urethane administration significantly increased the average number, size and diameter of tumors in PRDX4 Tg mice. Tumor apoptosis was suppressed and tumor proliferation was enhanced in Tg mice^[1].
Histology analysis: Urethane administration increased microvascular permeability and macrophage infiltration in lung tumor of PRDX4 Tg mice^[1].
Metabolic change: Urethane administration inhibited oxidative stress and decreased gene expressions of antioxidant enzymes (SOD1 and SOD2) in Tg mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 51-79-6
Appearance Solid
Molecular Weight 89.09
Formel C₃H₇NO₂
Color White to off-white
SMILES

NC(OCC)=O
Synonyms

Ethyl carbamate; Carbamic acid ethyl ester; Ethylurethane
Structure Classification
- Others
Initial Source
- Microorganisms
a byproduct of fermentation
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All

Sort by:

Journal Impact Factor

Sci Adv

Caspase-mediated LPS sensing and pyroptosis signaling in Hydra. [Abstract]

2023 Jul 21;9(29):eadh4054. PMID: 37478191
Antimicrob Agents Chemother

Impact of hepatic impairment and renal failure on the pharmacokinetics of linezolid and its metabolites: contribution of hepatic metabolism and renal excretion. [Abstract]

2025 Apr 14:e0189224. PMID: 40227039
ACS Omega

Matrix Stiffness Modulates Fibroblast Paracrine Signaling to Enhance Autophagy in Keratinocytes: Implications for Wound Healing. [Abstract]

2026 Mar 12;11(11):17605-17616. PMID: 41908360
Dev Comp Immunol

Hydra gasdermin-gated pyroptosis signalling regulates tissue regeneration. [Abstract]

2023 Dec:149:104904. PMID: 37543221

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (1122.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

11.2246 mL

56.1230 mL

112.2460 mL

5 mM

2.2449 mL

11.2246 mL

22.4492 mL

10 mM

1.1225 mL

5.6123 mL

11.2246 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (23.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (23.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.08 mg/mL (23.35 mM); Clear solution; Need warming

This protocol yields a clear solution of 2.08 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (561.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (617 KB)
- English - EN (617 KB)
- Français - FR (617 KB)
- Deutsch - DE (617 KB)
- Norwegian - NO (617 KB)
- Español - ES (617 KB)
- Swedish - SV (617 KB)
- Italian - IT (617 KB)
- Korean - KR (617 KB)
- Portuguese - PT (617 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zheng J, et al. Overexpression of PRDX4 Modulates Tumor Microenvironment and Promotes Urethane-Induced Lung Tumorigenesis. Oxid Med Cell Longev. 2020 Dec 28;2020:8262730. [Content Brief]

[2]. Sozio F, et al. Urethane-induced lung carcinogenesis. Methods Cell Biol. 2021;163:45-57. [Content Brief]

[3]. Gurley KE, et al. Induction of Lung Tumors in Mice with Urethane. Cold Spring Harb Protoc. 2015 Sep 1;2015(9):pdb.prot077446. [Content Brief]

[4]. K J Field, et al. Hazards of urethane (ethyl carbamate): a review of the literature. Lab Anim. 1988 Jul;22(3):255-62. [Content Brief]

[5]. R E Sotomayor, et al. Mutagenicity, metabolism, and DNA interactions of urethane. Toxicol Ind Health. 1990 Jan;6(1):71-108. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	11.2246 mL	56.1230 mL	112.2460 mL	280.6151 mL
	5 mM	2.2449 mL	11.2246 mL	22.4492 mL	56.1230 mL
	10 mM	1.1225 mL	5.6123 mL	11.2246 mL	28.0615 mL
	15 mM	0.7483 mL	3.7415 mL	7.4831 mL	18.7077 mL
	20 mM	0.5612 mL	2.8062 mL	5.6123 mL	14.0308 mL
	25 mM	0.4490 mL	2.2449 mL	4.4898 mL	11.2246 mL
	30 mM	0.3742 mL	1.8708 mL	3.7415 mL	9.3538 mL
	40 mM	0.2806 mL	1.4031 mL	2.8062 mL	7.0154 mL
	50 mM	0.2245 mL	1.1225 mL	2.2449 mL	5.6123 mL
	60 mM	0.1871 mL	0.9354 mL	1.8708 mL	4.6769 mL
	80 mM	0.1403 mL	0.7015 mL	1.4031 mL	3.5077 mL
	100 mM	0.1122 mL	0.5612 mL	1.1225 mL	2.8062 mL

Keywords

Urethane

4 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Urethane

Anti-Infection Compound Library

Natural Product Library

Anti-Cancer Compound Library

Clinical Compound Library

Anti-Aging Compound Library

Drug Repurposing Compound Library

Oxygen Sensing Compound Library

Antibacterial Compound Library

Medicine Food Homology Compound Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antiparasitic Compound library

Microbial Metabolite Library

Animal Disease Model Inducer Library

Tumorigenesis-Related Compound Library

Anti-Cancer Natural Product Library

Human Metabolite Library

Withdrawn Drug Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Chinese Baijiu Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Environmental Pollutant Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Urethane

Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Urethane Related Classifications

HY-B1207 Related Classifications