Pulchinenoside E2
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Pulchinenoside E2 is a triterpenoid saponin. Pulchinenoside E2 inhibits the phosphorylation of STAT3 and JAK2, blocks the nuclear translocation and transcriptional activity of STAT3, and suppresses STAT3-dependent mitochondrial oxidative phosphorylation. Pulchinenoside E2 inhibits invasion, migration and autophagy of triple-negative breast cancer cells. Pulchinenoside E2 can be used in research related to triple-negative breast cancer.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.28%
- CAS. Nr.: 244202-36-6
- Formel: C53H86O21
- Molecular Weight:1059.24
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Speicherung:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
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JAK2 |
STAT3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
5.9 μM
Compound: 9
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Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
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[PMID: 23639650] |
| A549 | IC50 |
7.9 μM
Compound: 9
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Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
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[PMID: 23639650] |
| HL-60 | IC50 |
2.6 μg/mL
Compound: 8
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Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
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[PMID: 10514313] |
| HL-60 | IC50 |
3.5 μM
Compound: 9
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Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
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[PMID: 23639650] |
Pulchinenoside E2 (0.75-1.5 μM; 24 h) induces lysosomal dysfunction in HS-578T and MDA-MB-231 TNBC cells, characterized by suppressed proteolytic activity, time-dependent degradation of mature CTSD, and lysosomal alkalinization[1].
Pulchinenoside E2 (0.75-1.5 μM; 24 h) dose-dependently increases GFP-LC3B puncta formation and impairs autophagic flux in HS-578T and MDA-MB-231 TNBC cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HS-578T, MDA-MB-231 TNBC cells
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Concentration:0.75, 1.5 μM
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Incubation Time:24 h
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Result:Dose-dependently increased the number of autophagic puncta and fluorescence intensity of GFP-LC3B in both cell lines.
Dose-dependently increased the LC3II/LC3I ratio and accumulation of the autophagy substrate p62.
| Species | Dose | Route | Tmax | Cmax | T1/2 | AUC0-t | MRTlast |
|---|---|---|---|---|---|---|---|
| Mice[1] | 5 mg/kg | i.p. | 6.30 h | 35.70 μg/mL | >12 h | 338.80 μg·h/mL | 6.67 h |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:M-NSG mice (female, 4-6 weeks old, tail vein injection of MDA-MB-231 cells to induce metastases)[2]
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Dosage:5 mg/kg; 10 mg/kg
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Administration:i.p.; alternate days; 30 days
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Result:Markedly reduced pulmonary and hepatic metastatic lesion numbers and tumor volumes in mice treated with 10 mg/kg compared to controls.
Significantly decreased phosphorylated STAT3 (Tyr705/Ser727) expression in metastatic tumors via immunohistochemical staining.
Accumulated LC3B and p62 in metastatic tumors, consistent with autophagic flux blockade.
Maintained serum AST, ALT, CRE, and BUN levels comparable to controls.
Showed no significant histopathological damage in heart and kidney tissues.
Maintained stable body weight throughout treatment, indicating no overt toxicity.
Chemical Information
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CAS. Nr. 244202-36-6
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Appearance Solid
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Molecular Weight 1059.24
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Formel C53H86O21
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Color White to off-white
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SMILES
CC1(C)[C@@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](C)O3)[C@@H](O)[C@@H](O)CO2)CC[C@@]6(C)[C@@]1([H])CC[C@]7(C)[C@]6([H])CC=C8[C@@]7(C)CC[C@]9(C(O)=O)[C@@]8([H])CC(C)(C)CC9
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Reinheit & Dokumentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Pulchinenoside E2 Related Classifications
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