Art. -Nr.: HY-123838

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Ro60-0175

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Ro60-0175 is a potent and selective agonist of 5-HT_2C receptor. Ro60-0175 reduces self-administration.

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Reinheit: 99.52%
CAS. Nr.: 169675-08-5
Formel: C₁₁H₁₂ClFN₂
Molecular Weight:226.68

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ro60-0175 Chemical Structure

CAS. Nr.: 169675-08-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Ro60-0175 is a potent and selective agonist of 5-HT_2C receptor. Ro60-0175 reduces self-administration^[1].

IC₅₀ & Target

5-HT_2C Receptor

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	131 nM Compound: 2 (Ro-600175)	Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2A receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	131 nM Compound: 4, Ro-600175	Binding to human 5HT2A receptor expressed in CHO cells Binding to human 5HT2A receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	18 nM Compound: 2 (Ro-600175)	Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2C receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	18 nM Compound: 4, Ro-600175	Binding to human 5HT2C receptor expressed in CHO cells Binding to human 5HT2C receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	4.3 nM Compound: 2 (Ro-600175)	Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader Functional activity against human 5-hydroxytryptamine 2B receptor expressed in CHO cells using fluorometric imaging plate reader	[PMID: 15081042]
CHO	EC₅₀	4.3 nM Compound: 4, Ro-600175	Binding to human 5HT2B receptor expressed in CHO cells Binding to human 5HT2B receptor expressed in CHO cells	[PMID: 16257207]
CHO	EC₅₀	400 nM Compound: 1, Ro-600175	Agonist activity at human cloned 5HT2A receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2A receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]
CHO	EC₅₀	52 nM Compound: 1, Ro-600175	Agonist activity at human cloned 5HT2C receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2C receptor expressed in CHO cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]
HEK293-EBNA	EC₅₀	2.4 nM Compound: 1, Ro-600175	Agonist activity at human cloned 5HT2B receptor expressed in HEK293-EBNA cells assessed as myo-[3H]inositol hydrolysis Agonist activity at human cloned 5HT2B receptor expressed in HEK293-EBNA cells assessed as myo-[3H]inositol hydrolysis	[PMID: 18035544]

In Vivo

Ro60-0175 (1 mg/kg; s.c.) preserves the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reach their break-points earlier^[1].
Ro60-0175 (0.3, 1, and 3 mg/kg; s.c.) significantly reduces responding on the active lever in the reinstatement group^[1].
Ro60-0175 (0.5 mg/kg SB242084; 1 mg/kg Ro60-0175; s.c.; i.p.) reduces responding compared to vehicle in the reinstatement group, and that this effect is prevented by pretreatment with SB242084. For responding on the inactive lever, there are no significant main effects or interactions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male Sprague-Dawley rats (280-320 g)^[1]
Dosage:

1 mg/kg
Administration:

s.c.
Result:

Preserved the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reached their break-points earlier.

Animal Model:

Adult male Sprague-Dawley rats (280-320 g)^[1]
Dosage:

0.5 mg/kg (SB242084), 1 mg/kg (Ro60-0175)
Administration:

s.c. (Ro60-0175), i.p. (SB242084)
Result:

Reduced responding for cocaine and effect was reversed by SB242084.

Animal Model:

Adult male Sprague-Dawley rats (280-320 g)^[1]
Dosage:

0.3, 1, and 3 mg/kg (Ro60-0175), 1 mg/kg (yohimbine)
Administration:

s.c. (Ro60-0175), i.p. (yohimbine)
Result:

Showed yohimbine treatment alone increased responding relative to vehicle injection, and the response was attenuated dose dependently by Ro60-0175.

Animal Model:

Adult male Sprague-Dawley rats (280-320 g)^[1]
Dosage:

0.5 mg/kg (SB242084), 1 mg/kg (Ro60-0175), 1 mg/kg (yohimbine)
Administration:

s.c. (Ro60-0175), i.p. (yohimbine), i.p. (SB242084)
Result:

Ro60-0175 reduced responding and that this effect was prevented by SB242084 pretreatment.

Chemical Information

CAS. Nr. 169675-08-5
Appearance Solid
Molecular Weight 226.68
Formel C₁₁H₁₂ClFN₂
Color White to light yellow
SMILES

N[C@@H](C)CN1C=CC2=C1C=C(Cl)C(F)=C2
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (1102.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.4115 mL

22.0573 mL

44.1147 mL

5 mM

0.8823 mL

4.4115 mL

8.8229 mL

10 mM

0.4411 mL

2.2057 mL

4.4115 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Fletcher PJ, et al. The 5-HT2C receptor agonist Ro60-0175 reduces cocaine self-administration and reinstatement induced by the stressor yohimbine, and contextual cues. Neuropsychopharmacology. 2008;33(6):1402-1412. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.4115 mL	22.0573 mL	44.1147 mL	110.2867 mL
	5 mM	0.8823 mL	4.4115 mL	8.8229 mL	22.0573 mL
	10 mM	0.4411 mL	2.2057 mL	4.4115 mL	11.0287 mL
	15 mM	0.2941 mL	1.4705 mL	2.9410 mL	7.3524 mL
	20 mM	0.2206 mL	1.1029 mL	2.2057 mL	5.5143 mL
	25 mM	0.1765 mL	0.8823 mL	1.7646 mL	4.4115 mL
	30 mM	0.1470 mL	0.7352 mL	1.4705 mL	3.6762 mL
	40 mM	0.1103 mL	0.5514 mL	1.1029 mL	2.7572 mL
	50 mM	0.0882 mL	0.4411 mL	0.8823 mL	2.2057 mL
	60 mM	0.0735 mL	0.3676 mL	0.7352 mL	1.8381 mL
	80 mM	0.0551 mL	0.2757 mL	0.5514 mL	1.3786 mL
	100 mM	0.0441 mL	0.2206 mL	0.4411 mL	1.1029 mL

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Ro60-0175

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Coagulation and Anti-coagulation Compound Library

Membrane Protein-targeted Compound Library

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Multi-Target Compound Library

Bioactive Compound Library Max

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Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

3D Diverse Fragment Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

CNS-Penetrant Compound Library

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Ro60-0175

Neurotransmitter Receptor Compound Library

Anti-Alzheimer's Disease Compound Library

Anti-Obesity Compound Library

Antidepressant Compound Library

5-HT Receptor Compound Library

Coagulation and Anti-coagulation Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

3D Diverse Fragment Library

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

CNS-Penetrant Compound Library

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Ro60-0175 Related Classifications

HY-123838 Related Classifications