Cat. No.: HY-W012595

Benzylideneacetone

(Synonyms: Benzalacetone)

Customer Review

Based on 1 Customer Validation

Benzylideneacetone (Benzalacetone) is an orally active antibiotic, tyrosinase inhibitor, phospholipase A₂ inhibitor, and immunosuppressant. Benzylideneacetone has antibacterial activity against some gram-negative plant-pathogenic bacteria. Benzylideneacetone can also be used in the synthesis of chemicals and drugs, and as a flavoring additive for some foods.

For research use only. We do not sell to patients.

Purity: 99.66%
CAS No.: 122-57-6
Formula: C₁₀H₁₀O
Molecular Weight:146.19

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Benzylideneacetone Chemical Structure

CAS No.: 122-57-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

All Phospholipase Isoforms :

All Endogenous Metabolite Isoforms :

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.8 μg/mL Compound: 9	Cytotoxicity against human A549 cells after 2 days by sulforhodamine B assay Cytotoxicity against human A549 cells after 2 days by sulforhodamine B assay	[PMID: 17067159]
HeLa	IC₅₀	>20 μM Compound: Butenone	Antiproliferative activity against human HeLa cells after 72 hrs by trypan blue assay Antiproliferative activity against human HeLa cells after 72 hrs by trypan blue assay	[PMID: 19155103]
HL-60	IC₅₀	>20 μM Compound: Butenone	Antiproliferative activity against human HL60 cells after 72 hrs by trypan blue assay Antiproliferative activity against human HL60 cells after 72 hrs by trypan blue assay	[PMID: 19155103]
K562	IC₅₀	17 μM Compound: 4v	In vitro cell growth inhibitory activity against K562 human chronic myelogenous leukemia cell line In vitro cell growth inhibitory activity against K562 human chronic myelogenous leukemia cell line	10.1016/S0960-894X(97)10147-0
KB	IC₅₀	5.3 μg/mL Compound: 9	Cytotoxicity against human KB cells after 2 days by sulforhodamine B assay Cytotoxicity against human KB cells after 2 days by sulforhodamine B assay	[PMID: 17067159]
KB	IC₅₀	5.6 μg/mL Compound: 9	Cytotoxicity against multidrug-resistant human KB-VCR cells after 2 days by sulforhodamine B assay Cytotoxicity against multidrug-resistant human KB-VCR cells after 2 days by sulforhodamine B assay	[PMID: 17067159]
Raji	IC₅₀	129 molar ratio Compound: 46	Cytotoxicity against human Raji cells expressing EBV-EA assessed as inhibition of TPA-induced EBV-EA activation after 48 hrs by trypan blue staining based immunofluorescence method relative to TPA Cytotoxicity against human Raji cells expressing EBV-EA assessed as inhibition of TPA-induced EBV-EA activation after 48 hrs by trypan blue staining based immunofluorescence method relative to TPA	[PMID: 26796952]

In Vitro

Benzylideneacetone (10000 ppm) exhibits significant antibacterial activity against Agrobacterium vitis, Pectobacterium carotovorum subsp. atrosepticum, P. carotovorum subsp. carotovorum, Pseudomonas syringae pv. tabaci and Ralstonia solanacearum, etc.^[1]
Benzylideneacetone (2-48 h) maintains its antibacterial activity at 65 °C^[1].
Benzylideneacetone (0-5 mM) can effectively inhibit the activity of mushroom tyrosinase, with an IC₅₀ of 1.5 mM for monophenolase and 2.0 mM for diphenolase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Benzylideneacetone (250 μM; oral administration; 48 h) enhances the virulence of Bacillus thuringiensis in the fifth-instar larvae of Spodoptera exigua^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 122-57-6
Appearance <39°C Solid,>42°C Liquid
Molecular Weight 146.19
Formula C₁₀H₁₀O
Color Colorless to white
SMILES

CC(/C=C/C1=CC=CC=C1)=O
Synonyms

Benzalacetone
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Microorganisms
- Endogenous metabolite
entomopathogenic bacterium, Xenorhabdus nematophila
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (684.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.8404 mL

34.2021 mL

68.4041 mL

5 mM

1.3681 mL

6.8404 mL

13.6808 mL

10 mM

0.6840 mL

3.4202 mL

6.8404 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ji D, et al. Identification of an antibacterial compound, benzylideneacetone, from Xenorhabdus nematophila against major plant-pathogenic bacteria. FEMS Microbiol Lett. 2004 Oct 15;239(2):241-8. [Content Brief]

[2]. Liu X, et al. Inhibition effects of benzylideneacetone, benzylacetone, and 4-phenyl-2-butanol on the activity of mushroom tyrosinase. J Biosci Bioeng. 2015 Mar;119(3):275-9. [Content Brief]

[3]. Kwon B, et al. Benzylideneacetone, an immunosuppressant, enhances virulence of Bacillus thuringiensis against beet armyworm (Lepidoptera: Noctuidae). J Econ Entomol. 2008 Feb;101(1):36-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.8404 mL	34.2021 mL	68.4041 mL	171.0103 mL
	5 mM	1.3681 mL	6.8404 mL	13.6808 mL	34.2021 mL
	10 mM	0.6840 mL	3.4202 mL	6.8404 mL	17.1010 mL
	15 mM	0.4560 mL	2.2801 mL	4.5603 mL	11.4007 mL
	20 mM	0.3420 mL	1.7101 mL	3.4202 mL	8.5505 mL
	25 mM	0.2736 mL	1.3681 mL	2.7362 mL	6.8404 mL
	30 mM	0.2280 mL	1.1401 mL	2.2801 mL	5.7003 mL
	40 mM	0.1710 mL	0.8551 mL	1.7101 mL	4.2753 mL
	50 mM	0.1368 mL	0.6840 mL	1.3681 mL	3.4202 mL
	60 mM	0.1140 mL	0.5700 mL	1.1401 mL	2.8502 mL
	80 mM	0.0855 mL	0.4275 mL	0.8551 mL	2.1376 mL
	100 mM	0.0684 mL	0.3420 mL	0.6840 mL	1.7101 mL

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Benzylideneacetone

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All Phospholipase Isoforms

All Endogenous Metabolite Isoforms

All Antibiotic Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Benzylideneacetone Related Classifications

HY-W012595 Related Classifications