Denikitug
Based on 1 Customer Validation
Denikitug (GS-1811; JTX-1811) is a humanized monoclonal antibody against CCR8 receptor with a KD of 16.8 pM. Denikitug specifically binds to human CCR8, inhibits CCL1-induced downstream CCR8 signaling. Denikitug selectively depletes cells expressing CCR8 via antibody-dependent cellular cytotoxicity (ADCC). Denikitug promotes anti-tumor immunity and can be used for the research of cancer and immunology.
For research use only. We do not sell to patients.
- Purity : 99.00%
- CAS No.: 2865822-82-6
- Molecular Weight:147.2 kDa
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Storage:Shipping with dry ice
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
[1]|
CCR8 16.8 pM (Kd) |
In Vitro
Denikitug exhibits high on-cell affinity for human CCR8, with an average KD of 16.8 pM[1].
Denikitug (0.0001-100 µg/mL) specifically binds to human CCR8-expressing CHO-S cells and does not bind to human CCR4-expressing or parental CHO-S cells[1].
Denikitug (0.001-100 µg/mL) potently antagonizes CCL1-induced signaling downstream of human CCR8[1].
Denikitug (10-4-100 µg/mL, overnight) mediates potent ADCC of cells expressing human CCR8, with an average EC50 of 0.0067 µg/mL[1].
Denikitug (10-6-1000 µg/mL, overnight) potently mediates ADCC of Hut78 cells endogenously expressing human CCR8 at tumor-infiltrating Treg levels, with an average EC50 of 0.0017 µg/mL and maximum killing of 67.5%[1].
Denikitug (1 µg/mL) mediates specific depletion of tumor-infiltrating Tregs ex vivo from human ovarian, lung, and colon tumors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CHO-S cells expressing 2500 copies of human CCR8 per cell, human NK cells isolated from healthy donors
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Concentration:10-8-100 µg/mL
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Incubation Time:overnight
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Result:Exhibited potent ADCC activity, with an average EC50 of 0.0067 µg/mL (range: 0.00094-0.023 µg/mL) across 10 independent NK cell donors.
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Cell Line:Hut78 cells (endogenously expressing human CCR8 at tumor-infiltrating Treg levels), human NK cells isolated from healthy donors
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Concentration:10-6-1000 µg/mL
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Incubation Time:overnight
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Result:Had an average EC50 of 0.0017 µg/mL and an average maximum killing of 67.5% across three NK cell donors.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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IgG1-kappa
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow Cytometry analysis of Raji cells labelling CCR8 (red) with Denikitug (HY-P990914). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2865822-82-6
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Appearance Liquid
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Molecular Weight 147.2 kDa
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Color Colorless to light yellow
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SMILES
[Denikitug]
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Synonyms
GS-1811; JTX-1811
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Denikitug Related Classifications
HY-P990914 Related Classifications
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