Calphostin B
Calphostin B is a protein kinase C (PKC) inhibitor with an IC50 of 1.04 μM in rats and an IC50 of 22.9 μM for bovine cyclic adenosine monophosphate-dependent protein kinase (PKA). Calphostin B bears aromatic structures at positions C-14 and C-17, which accounts for its higher selectivity for PKC over PKA. Calphostin B can be used in studies related to human cervical cancer and human breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 124824-06-2
- Formula: C37H34O11
- Molecular Weight:654.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
PKC 1.04 μM (IC50) |
PKA 22.9 μM (IC50) |
In Vitro
Calphostin B inhibits partially purified rat brain PKC with an IC50 of 1.04 μM and partially purified bovine heart PKA with an IC50 of 22.9 μM[1].
Calphostin B (1 h) inhibits the growth of human cervical cancer HeLa S3 cells with an IC50 of 2.56 μM and human breast cancer MCF-7 cells with an IC50 of 1.61 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 124824-06-2
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Molecular Weight 654.66
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Formula C37H34O11
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SMILES
O=C(C1=CC=CC=C1)OC(CC2=C(C(C3=C(C=C(C(C4=C5C6=C(O)C=C4OC)=C3C2=C5C(CC(C)O)=C(OC)C6=O)OC)O)=O)OC)C
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Structure Classification
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Initial Source
Cladosporium cladosporioides
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Calphostin B Related Classifications
HY-N19702 Related Classifications
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