Cat. No.: HY-120269

CAY10512

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CAY10512 is a NF-κB inhibitor. CAY10512 can suppress the upregulation of NF-κB-sensitive proinflammatory miRNAs (miRNA-9, miRNA-125b, miRNA-146a, miRNA-155) in cerebrospinal fluid and extracellular fluid. CAY10512 significantly reduces the release of pro-inflammatory cytokines (such as TNF-α, MCP-1, IL-8, IL-6). CAY10512 can be used for researchs on neuroinflammation, islet transplantation and microRNA regulation.

For research use only. We do not sell to patients.

Purity: 97.0%
CAS No.: 139141-12-1
Formula: C₁₅H₁₃FO
Molecular Weight:228.26

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

CAY10512 Chemical Structure

CAS No.: 139141-12-1

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(In Japan)

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]^[3]

NF-κB

IL-6

IL-8

All NF-κB Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	0.15 μM Compound: 6p	Inhibition of TNF-alpha-induced NF-kappaB activation in HEK293T cells Inhibition of TNF-alpha-induced NF-kappaB activation in HEK293T cells	[PMID: 17125270]
HepG2	EC₅₀	5.4 μM Compound: 1; LD55	Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay	[PMID: 28126440]

In Vitro

CAY10512 (5 μM, 0-96 h) effectively inhibits miRNA-9, miRNA-125b, miRNA146a and miRNA-155 upregulation in HNG cells stimulated by AD-ECF^[1].
CAY10512 (0.15 μM, 1-7 h) significantly protects pancreatic islet viability and effectively alleviates pancreatic islet cell damage by inhibiting the increase of pro-inflammatory factors and the expression of tissue factor TF in primary human islets^[2].
CAY10512 (0.25 μM, 25 h) significantly inhibits the gene and protein expressions of pro-inflammatory factor IL-6 and inflammatory receptor TNFR2 induced by MR agonists (Aldosterone (HY-113313), 11-dehydrocorticosterone (HY-113447)) in BV-2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[2]

Cell Line:

Primary human islets
Concentration:

0.15 μM
Incubation Time:

Pre-treat for one hour, then mix with autologous blood and incubate for another 1, 3, 6 h
Result:

Prevented the increase of TNF-α, MCP-1, IL-8 and IL-6.

RT-PCR^[3]

Cell Line:

BV-2 cells
Concentration:

0.25 μM
Incubation Time:

Pre-treatment for 1 hour, then add the stimulus and incubate for 24 hours
Result:

Completely blocked the upregulation of IL-6 mRNA induced by Aldosterone and the upregulation of TNFR2 mRNA induced by 11-dehydrocorticosterone.

Western Blot Analysis^[3]

Cell Line:

BV-2 cells
Concentration:

0.25 μM
Incubation Time:

Pre-treatment for 1 hour, then add the stimulus and incubate for 24 hours
Result:

Completely blocked the phosphorylation of ILp65 induced by aldosterone and its translocation to the nucleus, as well as the upregulation of TNFR2 protein levels induced by 11-dehydrocorticosterone.

Chemical Information

CAS No. 139141-12-1
Appearance Solid
Molecular Weight 228.26
Formula C₁₅H₁₃FO
Color White to off-white
SMILES

COC(C=C1)=CC=C1/C=C/C2=C(C=CC=C2)F
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Alexandrov P N, et al. microRNA (miRNA) speciation in Alzheimer’s disease (AD) cerebrospinal fluid (CSF) and extracellular fluid (ECF)[J]. International journal of biochemistry and molecular biology, 2012, 3(4): 365. [Content Brief]

[2]. Kanak MA, et al. Alleviation of instant blood-mediated inflammatory reaction in autologous conditions through treatment of human islets with NF-κB inhibitors. Transplantation. 2014 Sep 15;98(5):578-84. [Content Brief]

[3]. Chantong B, et al. Mineralocorticoid and glucocorticoid receptors differentially regulate NF-kappaB activity and pro-inflammatory cytokine production in murine BV-2 microglial cells. J Neuroinflammation. 2012 Nov 28;9:260. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

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CAY10512

Apoptosis Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Chemokine Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cytokine Inhibitors Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

All NF-κB Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms