Art. -Nr.: HY-N1458

Isoschaftoside

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, can inhibit the growth of germinated S. hermonthica radicles. Isoschaftoside reduces reactive oxygen species (ROS) and induces proliferation in senescent cells. Isoschaftoside activates autophagy. Isoschaftoside can be used for anti-tumor, anti-inflammatory, antioxidant, antihypertensive, hepatoprotective and nematicidal study.

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Reinheit: 99.95%
CAS. Nr.: 52012-29-0
Formel: C₂₆H₂₈O₁₄
Molecular Weight:564.49

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isoschaftoside Chemical Structure

CAS. Nr.: 52012-29-0

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

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In Vitro

Isoschaftoside (4 μM, 12 days) suppresses ROS by reducing the expression of RAC2 and LINC00294 in senescent fibroblasts^[1].
Isoschaftoside (0.25-4 μM, 12 days) significantly increases cell proliferation in senescent fibroblasts^[1].
Isoschaftoside (1 μM, 12 days) significantly increases mitochondrial membrane potential (MMP), induces metabolic changes that restore mitochondrial function and reduces dependence on glycolysis in senescent fibroblasts^[1].
Isoschaftoside (1 μM, 12 days) reduces DNA damage and fragmentation, activates the autophagy system to clear senescent fibroblasts, reduces the expression of inflammatory factors in senescent fibroblasts^[1].
Isoschaftoside (20-600 μg/mL) possesses strong nematicidal activity against M. incognita (LC₅₀ = 323.09 μg/mL)^[2].
Isoschaftoside (1-500 μM, 24 h) reduces lipid deposition via activating autophagy flux, and suppresses the expression of light-chain 3-II (LC3-II) and SQSTM1/p62 in Palmitic acid (PA) (HY-N0830)-induced autophagy inhibition in HepG2 cells^[3].
Isoschaftoside (0.05-2 mg/mL) considerably decreases renal Na+, K+-ATPase activation in basolateral membrane of the proximal tubule from pig kidney^[4].
Isoschaftoside (0-1000 μM, 9-24 h) can suppress inflammatory responses in Lipopolysaccharides (HY-D1056) (LPS)-activated microglia^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

human dermal fibroblasts
Concentration:

0.25, 0.5, 1, 2, 4 μM
Incubation Time:

12 days
Result:

The increase in cell proliferation rate was most significant at a concentration of 1 μM.

Cell Autophagy Assay^[1]

Cell Line:

human dermal fibroblasts
Concentration:

1 μM
Incubation Time:

12 days
Result:

LC3B was noticeably activated.
Perinuclear mitochondria were reduced.
Showed significantly enhanced levels of autophagy.
Significant reduction in lipofuscin was observed.

Western Blot Analysis^[3]

Cell Line:

HepG2 cells
Concentration:

1, 10, 20, 50, 100, 200, 300, 400, 500 μM
Incubation Time:

24 h
Result:

When the concentration was higher than 200 μM, observed significant reductions in LC3-II expression.
Decreased LC3-II and p62 expression in Palmitic acid (PA) (HY-N0830)-induced autophagy.

Western Blot Analysis^[5]

Cell Line:

BV2 microglial cells
Concentration:

200 μM
Incubation Time:

9 h or 24 h
Result:

Significantly inhibited Lipopolysaccharides-induced iNOS, TNF-α, IL-1β, and COX2 protein expression.
Significantly reduced the Lipopolysaccharides-induced HIF-1α, HK2, and PFKFB3 expression.
Attenuated Lipopolysaccharides-induced phosphorylation of ERK1/2 and mTOR.

In Vivo

Isoschaftoside (20 mg/kg; i.p.; daily for 4 weeks) reverses the nonalcoholic fatty liver disease (NAFLD) and reduces hepatic steatosis in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57 mice fed with a high-fat diet (HFD) (4 weeks; male; C57BL/6JGpt)^[3]
Dosage:

20 mg/kg
Administration:

Intraperitoneal injection (i.p.); daily for 4 weeks
Result:

Significantly reduced the body weight and body size of the high-fat diet mice compared with the model group.
Reduced the visceral fat weight of high-fat diet mice.
Significantly attenuated hepatic lipid accumulation induced by a high-fat diet and decreased the number of intracellular lipid droplets and hepatocyte balloons.
Reversed the damage to liver function and the elevation of blood glucose caused by a high-fat diet.
The expression level of p62 and LC3-II was significantly lower than that in the HFD group.

Chemical Information

CAS. Nr. 52012-29-0
Appearance Solid
Molecular Weight 564.49
Formel C₂₆H₂₈O₁₄
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C([C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)C(O)=C([C@H]5[C@@H]([C@H]([C@H](CO5)O)O)O)C(O)=C13
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Planta Med

Bioaccessibility and Absorption of Flavonoid C-glycosides from Abrus mollis Using Simulated Digestion, Caco-2 Cell, and In Situ Single-pass Perfusion Models. [Abstract]

2021 Jun;87(7):570-580. PMID: 33545720

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (177.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7715 mL

8.8576 mL

17.7151 mL

5 mM

0.3543 mL

1.7715 mL

3.5430 mL

10 mM

0.1772 mL

0.8858 mL

1.7715 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lee YH, et al. Identification of Cellular Isoschaftoside-Mediated Anti-Senescence Mechanism in RAC2 and LINC00294. Molecules. 2024 Sep 4;29(17):4182. [Content Brief]

[2]. Du SS, et al. Nematocidal flavone-C-glycosides against the root-knot nematode (Meloidogyne incognita) from Arisaema erubescens tubers. Molecules. 2011 Jun 20;16(6):5079-86. [Content Brief]

[3]. Su Y, et al. Isoschaftoside Reverses Nonalcoholic Fatty Liver Disease via Activating Autophagy In Vivo and In Vitro. Evid Based Complement Alternat Med. 2022 Jun 27;2022:2122563. [Content Brief]

[4]. Anne Caroline Candido Gomes, et al. In vitro effect of isoschaftoside isolated from Syngonium podophyllum on pig kidney Na+, K+-ATPase. Quim. Nova 37 (10). 2014.

[5]. Guan S, et al. Isoschaftoside Inhibits Lipopolysaccharide-Induced Inflammation in Microglia through Regulation of HIF-1α-Mediated Metabolic Reprogramming. Evid Based Complement Alternat Med. 2022 Nov 23;2022:5227335. [Content Brief]

[6]. Hooper AM, et al. Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, is an allelochemical against the development of Striga. Phytochemistry. 2010 Jun;71(8-9):904-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7715 mL	8.8576 mL	17.7151 mL	44.2878 mL
	5 mM	0.3543 mL	1.7715 mL	3.5430 mL	8.8576 mL
	10 mM	0.1772 mL	0.8858 mL	1.7715 mL	4.4288 mL
	15 mM	0.1181 mL	0.5905 mL	1.1810 mL	2.9525 mL
	20 mM	0.0886 mL	0.4429 mL	0.8858 mL	2.2144 mL
	25 mM	0.0709 mL	0.3543 mL	0.7086 mL	1.7715 mL
	30 mM	0.0591 mL	0.2953 mL	0.5905 mL	1.4763 mL
	40 mM	0.0443 mL	0.2214 mL	0.4429 mL	1.1072 mL
	50 mM	0.0354 mL	0.1772 mL	0.3543 mL	0.8858 mL
	60 mM	0.0295 mL	0.1476 mL	0.2953 mL	0.7381 mL
	80 mM	0.0221 mL	0.1107 mL	0.2214 mL	0.5536 mL
	100 mM	0.0177 mL	0.0886 mL	0.1772 mL	0.4429 mL

Keywords

Isoschaftoside

1 Cited Publications

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Isoschaftoside

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Publications Citing Use of MedChemExpress (MCE) Isoschaftoside

Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Isoschaftoside Related Classifications

HY-N1458 Related Classifications