NHC-diphosphate
Based on 1 publication(s) in Google Scholar
NHC-diphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form. NHC is a pyrimidine ribonucleoside and behaves as a potent anti-virus agent. NHC effectively inhibits the replication of venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV) and hepatitis C virus (HCV).
For research use only. We do not sell to patients.
- Purity: 98.80%
- CAS No.: 39023-73-9
- Formula: C9H15N3O12P2
- Molecular Weight:419.18
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NHC-diphosphate
MoreAll Endogenous Metabolite Isoforms
MoreAll Topoisomerase Isoforms
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Biological Activity
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC.Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP; HY-135867D) can be observed, while NHC-triphosphate remains the most abundant metabolite.[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 39023-73-9
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Appearance Solid
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Molecular Weight 419.18
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Formula C9H15N3O12P2
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Color White to off-white
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SMILES
O[C@H]([C@@H]1O)[C@@](N(C=C/C2=N\O)C(N2)=O)([H])O[C@@H]1CO[P](O[P](O)(O)=O)(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
SARS-CoV-2 RdRp uses NDPs as a substrate and is able to incorporate NHC into RNA from diphosphate form molnupiravir. [Abstract]2023 Jan 31:226:946-955. PMID: 36528144
Solvent & Solubility
H2O : 160 mg/mL (381.70 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Brenda I Hernandez-Santiago, et al. Metabolism of the anti-hepatitis C virus nucleoside beta-D-N4-hydroxycytidine in different liver cells. Antimicrob Agents Chemother. 2004 Dec;48(12):4636-42 [Content Brief]
[2]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.3856 mL | 11.9281 mL | 23.8561 mL | 59.6403 mL |
| 5 mM | 0.4771 mL | 2.3856 mL | 4.7712 mL | 11.9281 mL | |
| 10 mM | 0.2386 mL | 1.1928 mL | 2.3856 mL | 5.9640 mL | |
| 15 mM | 0.1590 mL | 0.7952 mL | 1.5904 mL | 3.9760 mL | |
| 20 mM | 0.1193 mL | 0.5964 mL | 1.1928 mL | 2.9820 mL | |
| 25 mM | 0.0954 mL | 0.4771 mL | 0.9542 mL | 2.3856 mL | |
| 30 mM | 0.0795 mL | 0.3976 mL | 0.7952 mL | 1.9880 mL | |
| 40 mM | 0.0596 mL | 0.2982 mL | 0.5964 mL | 1.4910 mL | |
| 50 mM | 0.0477 mL | 0.2386 mL | 0.4771 mL | 1.1928 mL | |
| 60 mM | 0.0398 mL | 0.1988 mL | 0.3976 mL | 0.9940 mL | |
| 80 mM | 0.0298 mL | 0.1491 mL | 0.2982 mL | 0.7455 mL | |
| 100 mM | 0.0239 mL | 0.1193 mL | 0.2386 mL | 0.5964 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NHC-diphosphate Related Classifications
HY-135867D Related Classifications
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