CDK9-IN-48
CDK9-IN-48 is a CDK9 inhibitor with an IC50 of 1.37 nM. CDK9-IN-48 inhibits the proliferation of various cancer cells. CDK9-IN-48 can be used in the research of cancers such as acute myeloid leukemia and prostate cancer.
For research use only. We do not sell to patients.
- CAS No.: 2987897-20-9
- Formula: C23H24FN5O3S
- Molecular Weight:469.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CDK9 1.37 nM (IC50) |
In Vitro
CDK9-IN-48 (Example 18) (0.00000256-1 μM, 3 days) potently inhibits the proliferation of MOLM-13 acute myeloid leukemia cells, LNCaP prostate cancer cells, and MV-4-11 acute myeloid leukemia cells, with IC50 values of 0.645, 2.34, and 2.24 nM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MOLM-13, LNCaP, MV-4-11 cell
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Concentration:0.00000256-1μM, starting with 1 μM, a 5-fold serial dilution was performed to obtain 9 concentrations
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Incubation Time:2 days (post-compound addition, 37°C, 5% CO2)
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Result:Potently inhibited MOLM-13 cell proliferation with an IC50 of 0.645 nM.
Inhibited LNCaP cell proliferation with an IC50 of 2.34 nM.
Inhibited MV-4-11 cell proliferation with an IC50 of 2.24 nM.
Parmacokinetics
Chemical Information
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CAS No. 2987897-20-9
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Molecular Weight 469.53
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Formula C23H24FN5O3S
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SMILES
CN1C(C(C)(O)C)=C2C=C(C3=C(F)C=NC(NC4=CC(CS(C)(=O)=O)=CC=N4)=C3)C=CC2=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CDK9-IN-48 Related Classifications
HY-185339 Related Classifications
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