Cat. No.: HY-N0482

Phillyrin

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Phillyrin is isolated from Forsythia suspensa Vahl (Oleaceae), has antibacterial and anti-inflammatory activities. Phillyrin has potential inductive effects on rat CYP1A2 and CYP2D1 activities, without affecting CYP2C11 and CYP3A1/2 activities. Phillyrin has anti-influenza A virus activities.

For research use only. We do not sell to patients.

Purity: 98.82%
CAS No.: 487-41-2
Formula: C₂₇H₃₄O₁₁
Molecular Weight:534.55

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Phillyrin Chemical Structure

CAS No.: 487-41-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

CYP1

CYP2

All Cytochrome P450 Isoforms :

In Vitro

Phillyrin (1–5 μM; pretreatment 1 hour; 24 hours) inhibits high glucose-induced FAS protein significantly in HepG2 cells^[1].
Phillyrin (1–5 μM; pretreatment 1 hour; 24 hours) inhibits high glucose-induced FAS mRNA expression by suppressing SREBP-1c activation in human HepG2 hepatocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

Human HepG2 hepatocyte cells
Concentration:

1 μM; 2.5 μM; 5 μM
Incubation Time:

24 hours
Result:

Reduced FAS and SREBP-1c mRNA expression.

Chemical Information

CAS No. 487-41-2
Appearance Solid
Molecular Weight 534.55
Formula C₂₇H₃₄O₁₁
Color White to off-white
SMILES

COC(C=C1[C@@H]2[C@](CO[C@H]3C4=CC(OC)=C(OC)C=C4)([H])[C@]3([H])CO2)=C(C=C1)O[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO
Structure Classification
- Phenylpropanoids
- Lignans
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All Cell Proliferation/Viability AssayFlow CytometryCell Migration/Invasion AssayWBCell Imaging/Staining

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Journal Impact Factor

Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
Phytomedicine

Phillyrin promotes autophagosome formation in A53T-αSyn-induced Parkinson's disease model via modulation of REEP1. [Abstract]

2024 Aug 18:134:155952. PMID: 39178680
Int Immunopharmacol

Phillyrin: A potential therapeutic agent for osteoarthritis via modulation of NF-κB and Nrf2 signaling pathways. [Abstract]

2024 Aug 18:141:112960. PMID: 39159565
Front Cell Dev Biol

Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress. [Abstract]

2021 Nov 10;9:763864. PMID: 34858986
Food Sci Nutr

Network Pharmacology and Molecular Docking Elucidate the Mechanism of Phillyrin in Colorectal Cancer. [Abstract]

2025 Oct 23;13(10):e71069. PMID: 41142018

Phillyrin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2025 Oct 23;13(10):e71069. [Abstract]

The cell viability of HT29 and HCT116 was detected by CCK8 assays treated with Phillyrin.

Phillyrin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2025 Oct 23;13(10):e71069. [Abstract]

Flow cytometry of apoptosis was applied to detect apoptosis ratio of colorectal cancer cells after being incubated with Phillyrin (0.1, 0.2 mM).

Phillyrin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2025 Oct 23;13(10):e71069. [Abstract]

Wound healing assay demonstrated the effect of Phillyrin (0.1, 0.2 mM) on the migration ability of HT29 and HCT116.

Phillyrin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2025 Oct 23;13(10):e71069. [Abstract]

The western blot analysis revealed a downregulation in the expression of p‐PI3K, p‐AKT, and p‐mTOR in HT29 and HCT116 cells following treatment with Phillyrin (0.1, 0.2 mM).

Phillyrin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2025 Oct 23;13(10):e71069. [Abstract]

Colony formation assays displayed the proliferation of colorectal cancer cells was suppressed by Phillyrin (0.1, 0.2 mM) in a dose‐dependent manner.
J Dent Sci

2025 Dec 24.
J Orthop Surg Res

Phillyrin reduces ROS production to alleviate the progression of intervertebral disc degeneration by inhibiting NF-κB pathway. [Abstract]

2024 May 22;19(1):308. PMID: 38773639
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (467.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8707 mL

9.3537 mL

18.7073 mL

5 mM

0.3741 mL

1.8707 mL

3.7415 mL

10 mM

0.1871 mL

0.9354 mL

1.8707 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.17 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.17 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.17 mg/mL (4.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cheng Y, et al. Effects of phillyrin and forsythoside A on rat cytochrome P450 activities in vivo and in vitro. Xenobiotica. 2017 Apr;47(4):297-303. [Content Brief]

[2]. Qu XY, et al. Protective effects of phillyrin against influenza A virus in vivo. Arch Pharm Res. 2016 Jul;39(7):998-1005. [Content Brief]

[3]. Do MT, et al. Phillyrin attenuates high glucose-induced lipid accumulation in human HepG2 hepatocytes through the activation of LKB1/AMP-activated protein kinase-dependent signalling. Food Chem. 2013 Jan 15;136(2):415-25. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8707 mL	9.3537 mL	18.7073 mL	46.7683 mL
	5 mM	0.3741 mL	1.8707 mL	3.7415 mL	9.3537 mL
	10 mM	0.1871 mL	0.9354 mL	1.8707 mL	4.6768 mL
	15 mM	0.1247 mL	0.6236 mL	1.2472 mL	3.1179 mL
	20 mM	0.0935 mL	0.4677 mL	0.9354 mL	2.3384 mL
	25 mM	0.0748 mL	0.3741 mL	0.7483 mL	1.8707 mL
	30 mM	0.0624 mL	0.3118 mL	0.6236 mL	1.5589 mL
	40 mM	0.0468 mL	0.2338 mL	0.4677 mL	1.1692 mL
	50 mM	0.0374 mL	0.1871 mL	0.3741 mL	0.9354 mL
	60 mM	0.0312 mL	0.1559 mL	0.3118 mL	0.7795 mL
	80 mM	0.0234 mL	0.1169 mL	0.2338 mL	0.5846 mL
	100 mM	0.0187 mL	0.0935 mL	0.1871 mL	0.4677 mL

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Phillyrin

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Publications Citing Use of MedChemExpress (MCE) Phillyrin

All Cytochrome P450 Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phillyrin Related Classifications

Cat. No.: HY-N0482