Succinimide
Based on 1 Customer Validation
Succinimide is a modifying agent. Binding of Succinimide to the 5-HT7 receptor induces clathrin-mediated endocytosis. Succinimide-modified liposomes exhibit high cellular uptake. Succinimide can be used in the study of glioblastoma.
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- Pureza : 99.92%
- No. CAS: 123-56-8
- Fòrmula: C4H5NO2
- Peso molecular:99.09
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Ver todos los productos específicos de isoformas 5-HT Receptor
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Actividad biológica
Descripciòn
IC50 & Target
[3]|
5-HT7 Receptor |
In Vitro
Succinimide-coated liposomes (100-1000 μM; 1-3 h) show the highest cellular saturation amount S in mouse bEnd.3 brain endothelial cells, with S doubling from the value at 100 μM to 500 μM and increasing threefold from 100 μM to 1000 μM[3].
Succinimide-containing liposomes (1000 μM; 3 h) reduce viability to approximately 70% in mouse bEnd.3 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:murine bEnd.3 brain endothelial cells
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Concentration:100, 500, 1000 μM liposomal lipid
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Incubation Time:3 h (37 °C, 10% CO2)
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Result:At 1000 μM for 3 h, viability was reduced to around 70% with NHS-containing liposomes.
Chemical Information
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (1009.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (50.46 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (50.46 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (307 KB)
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SDS (393 KB)
- English - EN (393 KB)
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- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 10.0918 mL | 50.4592 mL | 100.9184 mL | 252.2959 mL |
| 5 mM | 2.0184 mL | 10.0918 mL | 20.1837 mL | 50.4592 mL | |
| 10 mM | 1.0092 mL | 5.0459 mL | 10.0918 mL | 25.2296 mL | |
| 15 mM | 0.6728 mL | 3.3639 mL | 6.7279 mL | 16.8197 mL | |
| 20 mM | 0.5046 mL | 2.5230 mL | 5.0459 mL | 12.6148 mL | |
| 25 mM | 0.4037 mL | 2.0184 mL | 4.0367 mL | 10.0918 mL | |
| 30 mM | 0.3364 mL | 1.6820 mL | 3.3639 mL | 8.4099 mL | |
| 40 mM | 0.2523 mL | 1.2615 mL | 2.5230 mL | 6.3074 mL | |
| 50 mM | 0.2018 mL | 1.0092 mL | 2.0184 mL | 5.0459 mL | |
| 60 mM | 0.1682 mL | 0.8410 mL | 1.6820 mL | 4.2049 mL | |
| 80 mM | 0.1261 mL | 0.6307 mL | 1.2615 mL | 3.1537 mL | |
| 100 mM | 0.1009 mL | 0.5046 mL | 1.0092 mL | 2.5230 mL |
Succinimide Related Classifications
HY-41877 Related Classifications
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