2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Anti-infection
  3. Estrogen Receptor/ERR Endogenous Metabolite Bacterial
  4. Estradiol hemihydrate

Estradiol hemihydrate  (Synonyms: β-Estradiol hemihydrate; 17β-Estradiol hemihydrate; 17β-Oestradiol hemihydrate)

Cat. No.: HY-B0141C Purity: 99.79%
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Estradiol (β-Estradiol) hemihydrate is a steroid hormone and the major female sex hormone. Estradiol (β-Estradiol) hemihydrate can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol (β-Estradiol) hemihydrate can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering.

For research use only. We do not sell to patients.

CAS No. : 35380-71-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
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Customer Review

Based on 122 publication(s) in Google Scholar

Other Forms of Estradiol hemihydrate:

Estradiol hemihydrate Related Antibodies

Top Publications Citing Use of Products

122 Publications Citing Use of MCE Estradiol hemihydrate

WB
Cell Proliferation/Viability Assay
RT-PCR

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2025 Feb;16(1):e13693.  [Abstract]

    Estradiol (E2) (10-100 nM) exhibites an increase in the SIRT1 protein level in C2C12 cells, without significantly affecting the mRNA level.

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 Jul 6:947:174543.  [Abstract]

    Estradiol (E2) (1 μM, 8 h) alone or in combination with TEB (100 μM, 8 h) (TEB + E2) significantly increases the protein expression levels of FoxL2 and pFoxL2 (Ser238) in ZF4 cells.

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 7:e14303.  [Abstract]

    Estradiol (3-25 ng/mouse; s.c.; for 7 consecutive days) significantly ​​decreases​​ LAMIN A levels while ​​increasing​​ PROGERIN and PRELAMIN A levels in the uteri of mice injected with 10 ng or 25 ng, compared to those receiving the 3 ng dose.

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2023 May 1:236:123987.  [Abstract]

    Estradiol (1-100 nM; 6-72 h) promotes the proliferation of sheep myoblasts.

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Aug 29;10(24):10874-10891.  [Abstract]

    Estradiol (E2) (100 nM; 36 h) treatment can enhance the expression of ERRα and VLDL-related genes such as Apob, Mttp and Pla2g12b, whereas the induction is blocked with loss of ERRα in primary hepatocytes.

    Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2021 Feb 1;270:116015.  [Abstract]

    Estradiol (1-10 nM; 24 h) significantly induces mRNA expression of both pS2 and NRF1 genes in MCF-7 cells.

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    Description

    Estradiol (β-Estradiol) hemihydrate is a steroid hormone and the major female sex hormone. Estradiol (β-Estradiol) hemihydrate can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol (β-Estradiol) hemihydrate can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering[1][2].

    In Vitro

    Estradiol hemihydrate (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)[1].
    Estradiol hemihydrate (17β-estradiol, 10 nM, 7 days) increases the expression of neuron-like cell markers (Tuj-1, nestin and NF-H) in neural-like cells differentiated from hEnSCs[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Estradiol hemihydrate Related Antibodies

    Cell Differentiation Assay[1]

    Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
    Concentration: 10 nM
    Incubation Time: 7 days
    Result: Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

    Immunofluorescence[1]

    Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
    Concentration: 10 nM
    Incubation Time: 7 days
    Result: Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.
    In Vivo

    Estradiol hemihydrate (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude[1].
    Estradiol hemihydrate (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: FBN-ARO-KO Mice[2]
    Dosage: 1 nM
    Administration: Treated for the hippocampal slices
    Result: Rescued long-term potentiation (LTP) amplitude of both male and female mice.
    Animal Model: FBN-ARO-KO Mice[2]
    Dosage: 0.0167 mg
    Administration: Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation.
    Result: Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain.
    Rescued the spatial learning and memory defects.
    Clinical Trial
    Molecular Weight

    281.39

    Formula

    C18H24O2·1/2H2O
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@](CC[C@@H]2O)([H])[C@@]3([H])[C@@](CC1)([H])C4=CC=C(O)C=C4CC3.O.[1/2]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (355.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5538 mL 17.7689 mL 35.5379 mL
    5 mM 0.7108 mL 3.5538 mL 7.1076 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    g

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.79%

    SDS (623 KB)

    COA (253 KB) HNMR (247 KB) RP-HPLC (282 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5538 mL 17.7689 mL 35.5379 mL 88.8447 mL
    5 mM 0.7108 mL 3.5538 mL 7.1076 mL 17.7689 mL
    10 mM 0.3554 mL 1.7769 mL 3.5538 mL 8.8845 mL
    15 mM 0.2369 mL 1.1846 mL 2.3692 mL 5.9230 mL
    20 mM 0.1777 mL 0.8884 mL 1.7769 mL 4.4422 mL
    25 mM 0.1422 mL 0.7108 mL 1.4215 mL 3.5538 mL
    30 mM 0.1185 mL 0.5923 mL 1.1846 mL 2.9615 mL
    40 mM 0.0888 mL 0.4442 mL 0.8884 mL 2.2211 mL
    50 mM 0.0711 mL 0.3554 mL 0.7108 mL 1.7769 mL
    60 mM 0.0592 mL 0.2961 mL 0.5923 mL 1.4807 mL
    80 mM 0.0444 mL 0.2221 mL 0.4442 mL 1.1106 mL
    100 mM 0.0355 mL 0.1777 mL 0.3554 mL 0.8884 mL
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    Estradiol hemihydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Estradiol hemihydrate35380-71-3β-Estradiol hemihydrate 17β-Estradiol hemihydrate 17β-Oestradiol hemihydrateEstrogen Receptor/ERREndogenous MetaboliteBacterialEstrogenneural differentiationhuman endometrial stem cellsFBN-ARO-KO modelsteroid sex hormoneuterine fibroidsInhibitorinhibitorinhibit

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