14-Deoxyandrographolide
Based on 1 publication(s) in Google Scholar
14-Deoxyandrographolide is a diterpene with calcium channel blocking activity and acts as a uterine smooth muscle relaxant. 14-Deoxyandrographolide stimulates the release of nitric oxide (NO) in endothelial cells. 14-Deoxyandrographolide gradually desensitizes liver cells to TNF-α mediated apoptosis by inducing the release of TNFRSF1A.
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- Pureza: 99.94%
- No. CAS: 4176-97-0
- Fòrmula: C20H30O4
- Peso molecular:334.45
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) 14-Deoxyandrographolide
MoreVer todos los productos específicos de isoformas Calcium Channel
MoreVer todos los productos específicos de isoformas Caspase
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Actividad biológica
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Caspase 3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | GI50 |
25.46 μM
Compound: 9
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Antiproliferative activity against human HL60 cells by trypan blue assay
Antiproliferative activity against human HL60 cells by trypan blue assay
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[PMID: 18357994] |
| NIH3T3 | IC50 |
176.3 μM
Compound: 5
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Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay
Antifibrotic activity against mouse NIH/3T3 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay
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[PMID: 30144698] |
| NTUB1 | IC50 |
>100 μM
Compound: 4
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Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay
Cytotoxicity against human NTUB1 cells after 48 hrs by MTT assay
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[PMID: 25913115] |
| RAW264.7 | IC50 |
21.01 μM
Compound: 1
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
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[PMID: 22264489] |
14-Deoxyandrographolide can reduce uterine smooth muscle contraction responses induced by 0.3 mM and 3.0 mM CaCl2, with IC50 values of 1.24 M and 5.94 M[1].
14-Deoxyandrographolide (0-15 nM, 1 h) shows no cytotoxicity in primary liver cells and prevents TNF-α induced cell death[2].
14-Deoxyandrographolide (5-15 nM, 1 h) inhibits the expression of caspase-3 in primary liver cells[2].
14-Deoxyandrographolide (10 nM, 1 h) suppressed the aggregation of TRADD, FADD and caspase-8 in TNF-a sensitized liver cells and inhibited the formation of the DISC[2].
14-Deoxyandrographolide (10 nM, 1 h) induces the release of TNFRSF1A from liver cells[2].
14-Deoxyandrographolide (0-100 μM, 24-48 h) stimulates endothelial cells to produce NO in a concentration-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary hepatocytes
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Concentration:0, 2.5, 5, 7.5, 10, 12.5, 15 nM
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Incubation Time:1 h
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Result:Reduced cell death rate.
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Cell Line:Primary hepatocytes
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Concentration:5, 10, 15 nM
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Incubation Time:1 h
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Result:Inhibited the expression of caspase-3 in a concentration-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats[2]
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Dosage:40 mg/kg; single dose
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Administration:Intraperitoneal injection (i.p.)
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Result:Reduced ALT elevation caused by TNF-α/ActD, reduced the number of hepatocyte apoptosis, and reduced the amount of TNF-α retained in the liver.
Chemical Information
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No. CAS 4176-97-0
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Appearance Solid
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Peso molecular 334.45
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Fòrmula C20H30O4
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Color White to off-white
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SMILES
O=C1OCC=C1CC[C@@H]2C(CC[C@]3([H])[C@](C)(CO)[C@H](O)CC[C@@]23C)=C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Eur J Pharmacol
Andrographolide analogues are agonists of the estrogen receptor alpha in vitro and in vivo. [Abstract]2026 Feb 28:1016:178641. PMID: 41643829
Solvente y solubilidad
DMSO : 100 mg/mL (299.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Burgos RA, et al. Effect of 14-deoxyandrographolide on calcium-mediated rat uterine smooth muscle contractility. Phytother Res. 2003;17(9):1011-1015. [Content Brief]
[2]. Roy DN, et al. 14-Deoxyandrographolide desensitizes hepatocytes to tumour necrosis factor-alpha-induced apoptosis through calcium-dependent tumour necrosis factor receptor superfamily member 1A release via the NO/cGMP pathway. Br J Pharmacol. 2010 Aug;160(7):1823-43. [Content Brief]
[3]. C Y Zhang, et al. Effects of 14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide on nitric oxide production in cultured human endothelial cells. Phytother Res. 1999 Mar;13(2):157-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9900 mL | 14.9499 mL | 29.8998 mL | 74.7496 mL |
| 5 mM | 0.5980 mL | 2.9900 mL | 5.9800 mL | 14.9499 mL | |
| 10 mM | 0.2990 mL | 1.4950 mL | 2.9900 mL | 7.4750 mL | |
| 15 mM | 0.1993 mL | 0.9967 mL | 1.9933 mL | 4.9833 mL | |
| 20 mM | 0.1495 mL | 0.7475 mL | 1.4950 mL | 3.7375 mL | |
| 25 mM | 0.1196 mL | 0.5980 mL | 1.1960 mL | 2.9900 mL | |
| 30 mM | 0.0997 mL | 0.4983 mL | 0.9967 mL | 2.4917 mL | |
| 40 mM | 0.0747 mL | 0.3737 mL | 0.7475 mL | 1.8687 mL | |
| 50 mM | 0.0598 mL | 0.2990 mL | 0.5980 mL | 1.4950 mL | |
| 60 mM | 0.0498 mL | 0.2492 mL | 0.4983 mL | 1.2458 mL | |
| 80 mM | 0.0374 mL | 0.1869 mL | 0.3737 mL | 0.9344 mL | |
| 100 mM | 0.0299 mL | 0.1495 mL | 0.2990 mL | 0.7475 mL |
14-Deoxyandrographolide Related Classifications
HY-N4323 Related Classifications
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