Referencia número: HY-A0107

Tetracycline

Name: Tetracycline
Brand: MedChemExpress
SKU: HY-A0107
Price: 25.00 USD
Availability: InStock
Rating: 5 (60 reviews)

60 Cited Publications

Revisión del cliente

Based on 60 publication(s) in Google Scholar

Tetracycline is a broad-spectrum antibiotic with oral activity. Tetracycline exhibits activity against a wide range of bacteria including gram-positive, gram-negative bacteria, chlamydiae, mycoplasmas and rickettsiae. Tetracycline can be used for the research of infections.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza : 98.0%
No. CAS: 60-54-8
Fòrmula: C₂₂H₂₄N₂O₈
Peso molecular:444.43

Almacenamiento:

4°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Tetracycline Chemical Structure

No. CAS: 60-54-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Induced Disease Models
- Ensayo clínico
Chemical Information
Publications (60)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

Tetracycline

Ver todos los productos específicos de isoformas Antibiotic :

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	CC₅₀	40.5 μM Compound: tetracycline	Cytotoxicity against CEM cells after 5 days by MTT method Cytotoxicity against CEM cells after 5 days by MTT method	[PMID: 17376679]
CCRF-CEM	EC₅₀	>40.5 μM Compound: tetracycline	Antiviral activity against HIV1 3B in CEM cells assessed as inhibition of viral-induced cytopathicity Antiviral activity against HIV1 3B in CEM cells assessed as inhibition of viral-induced cytopathicity	[PMID: 17376679]
HaCaT	IC₅₀	8 μM Compound: Tetracycline	Cytotoxicity against human HaCaT cells after 24 hrs by MTT assay Cytotoxicity against human HaCaT cells after 24 hrs by MTT assay	[PMID: 34223166]
HeLa	IC₅₀	>400 μg/mL Compound: Tetracycline	Antiproliferative effect against HeLa cells after 48 hrs Antiproliferative effect against HeLa cells after 48 hrs	[PMID: 17088489]
HeLa	IC₅₀	51 μM Compound: Tetracycline	Compound was evaluated for the inhibition of cell proliferation of human cervix epithelioid carcinoma, HeLa (ATCC CCL2). Compound was evaluated for the inhibition of cell proliferation of human cervix epithelioid carcinoma, HeLa (ATCC CCL2).	10.1016/0960-894X(96)00418-0
HepG2	IC₅₀	128 μM Compound: Tetracycline	Compound was evaluated for the inhibition of cell proliferation of human hepatocellular carcinoma, HepG2 (ATCC HB8065). Compound was evaluated for the inhibition of cell proliferation of human hepatocellular carcinoma, HepG2 (ATCC HB8065).	10.1016/0960-894X(96)00418-0
MCF7	IC₅₀	41.6 μM Compound: Tetracycline	Dark toxicity against human MCF7 cells assessed as decrease in cell viability by MTT assay Dark toxicity against human MCF7 cells assessed as decrease in cell viability by MTT assay	[PMID: 29673980]
MG-63	IC₅₀	180 μg/mL Compound: Tetracycline	Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs	[PMID: 17088489]
Osteoblast	IC₅₀	180 μg/mL Compound: Tetracycline	Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs	[PMID: 17088489]

In Vitro

Tetracycline shows susceptibilfty of V. vulnlflcus strain B3547 with MIC value of 0.5 g/mL^[2].
Tetracycline inhibits the L-amyloid aggregates formation and disassembles the pre-formed fibrils^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animal Model:

Female ICR mice weight 30 to 40 g with V.vulnificus strain B3547 infection^[2]
Dosage:

3 mg/kg
Administration:

Intraperitoneal injection; 3 mg/kg every 12h until survive
Result:

Inhibited the growth of human CNE-2 xenografts in nude mice.

Related Protocols :

Gram Staining of Tissue Sections

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Tetracycline can be used in animal modeling to create genetically engineered disease models. The pharmacokinetics of Tetracycline after intraperitoneal administration in broiler chickens exhibit unique distribution and elimination characteristics. Tetracycline distributes widely in the body, with rapid elimination, high clearance, and a large volume of distribution. After oral administration, Tetracycline shows high gastrointestinal absorption and moderate elimination rates. Although the absorption rate of Tetracycline is higher than that of most other tetracycline drugs, its overall drug exposure is relatively low^[4].

Induction of tumor regression based on BCR-ABL gene silencing^[4]

Background

The BCR-ABL gene encodes an abnormal tyrosine kinase (BCR-ABL protein), which leads to uncontrolled cell proliferation. Tetracycline can induce tumor regression in mice by inhibiting BCR-ABL expression through the suppression of the binding between tTA (tetracycline transactivator) and the Tet-OP (Tetracycline Operator) sequence^[4].

Specific Modeling Methods

Mice: nu/nu • female and male • 6-8 weeks old
Administration: 200 μg/ml • po • single dose

Note

1. Tetracycline is administered orally dissolved in drinking water containing 5% sucrose.
2. Mouse tumor model construction: To induce visible tumors, Ba/F3 cells transduced with SIN-p210 (a retrovirus carrying and inducing tetracycline-dependent BCR-ABL gene expression) are subcutaneously injected into nude mice.

Modeling Indicators

Phenotypic observation: The tumors established in the mice completely regress.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Chemical Information

No. CAS 60-54-8
Appearance Solid
Peso molecular 444.43
Fòrmula C₂₂H₂₄N₂O₈
Color Light yellow to yellow
SMILES

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@](C)(O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N
Structure Classification
- Phenols
- Monophenols
- Antibiotics
- Other Antibiotics
- Antibiotics
- Plant agriculture research
- Antibiotics
- Animal Husbandry
- Antibiotics
- Mammalian Cell Culture
Initial Source
- Microorganism
Shigella dysenteriae
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (60)

All Flow CytometryBio/Physico-chemical AssayCell Imaging/StainingMicrobiological AssayWB

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Journal Impact Factor

Cell Res

A novel protein RASON encoded by a lncRNA controls oncogenic RAS signaling in KRAS mutant cancers. [Abstract]

2023 Jan;33(1):30-45. PMID: 36241718
Cell Res

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. [Abstract]

2020 Dec;30(12):1063-1077. PMID: 32839552

Tetracycline purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]

Immunoblotting analysis of MLKL-Flag, tBid-Flag, and Cleaved Caspase-3 expression in cell death-inducible C2C12 cell lines with or without tetracycline treatment. C2C12-Mlkl-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 12 h to induce necroptosis, and C2C12-tBid-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 6 h to induce apoptosis.

Tetracycline purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]

Representative phase-contrast images of MuSCs cultured in different fractions of NCM after purification. The complete NCM was used as the positive control (NCM input); C2C12-Mlkl-TetON medium without Tetracycline hydrochloride treatment was used as the negative control (Tet-control). Cells were cultured for 48 h. Scale bars, 100 μm.

Tetracycline purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]

Immunoblotting analysis of the expression and extracellular release of TNC upon necroptosis induction at different time points as indicated. C2C12-Mlkl-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 12 h to induce necroptosis. Both the whole cell lysates and NCM were harvested and then subjected to immunoblotting analysis using antibodies as indicated.
Mater Today

2026 Apr 7;96:103326.
Mater Today

2025 Sep 12.
Cancer Res

HBx-induced HSPA8 stimulates HBV replication and suppresses ferroptosis to support liver cancer progression. [Abstract]

2023 Apr 4;83(7):1048-1061. PMID: 36745032
J Extracell Vesicles

Oxidative stress induces extracellular vesicle release by upregulation of HEXB to facilitate tumour growth in experimental hepatocellular carcinoma. [Abstract]

2024 Jul;13(7):e12468. PMID: 38944674

Tetracycline purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12468. [Abstract]

The protein levels of HEXB in HBV replication cell model. Left, Tetracycline was added to inhibit HBV replication in HepAD38 cells. Right, HepG2.2.15 was treated with NAC (1 mM, 12 h) to abolish ROS produced by HBV replication.
Nat Commun

DirectRM: integrated detection of landscape and crosstalk between multiple RNA modifications using direct RNA sequencing. [Abstract]

2025 Oct 27;16(1):9450. PMID: 41145497
Nat Commun

Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]

2022 Mar 2;13(1):1116. PMID: 35236849
J Nanobiotechnology

2026 Jul 30.
Nucleic Acids Res

Enhancing small-molecule-mediated translational control through multivalent RNA aptamers. [Abstract]

2026 Jun 8;54(11):gkag592. PMID: 42273917
J Clin Invest

Mycobacterium tuberculosis hijacks host TRIM21- and NCOA4-dependent ferritinophagy to enhance intracellular growth. [Abstract]

2023 Apr 17;133(8):e159941. PMID: 37066876

Tetracycline purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Apr 17;133(8):e159941. [Abstract]

THP-1–derived macrophages were infected for 72 hours with Mtb strain H37Ra carrying a dual-color reporter that comprises a constitutively green (Emerald) and a Tetracycline-inducible red (TagRFP) fluorescent protein. Tetracycline (500 ng/mL) was added 24 hours before analysis of the live or dead status of the H37Ra strain in macrophages by flow cytometry.
Adv Sci (Weinh)

A Macromolecule Reversing Antibiotic Resistance Phenotype and Repurposing Drugs as Potent Antibiotics. [Abstract]

2020 Jul 21;7(17):2001374. PMID: 32995131

Tetracycline purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jul 21;7(17):2001374. [Abstract]

The pEt_20 polymer mitigates resistance to Azithromycin, Gentamicin, Imipenem, and Tetracycline, and reduces their minimum inhibitory concentration (MIC) to sensitive levels or even lower.
Research (Wash D C)

Bacillus siamensis Targeted Screening from Highly Colitis-Resistant Pigs Can Alleviate Ulcerative Colitis in Mice. [Abstract]

2024 Jul 16:7:0415. PMID: 39015206
Water Res

Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]

2023 Jul 15:240:120110. PMID: 37247434
Chem Eng J

2023 Oct 15, 474, 145978.
EBioMedicine

A novel inhibitor of monooxygenase reversed the activity of tetracyclines against tet(X3)/tet(X4)-positive bacteria. [Abstract]

2022 Apr;78:103943. PMID: 35306337

Tetracycline purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]

Microdilution checkerboard analysis showed that plumbagin restored the susceptibility of tet(X4)-carrying E. coli J53p47EC and K. pneumoniae K12016p47EC to Tetracycline, in comparison to the tet(X4)-negative strains E. coli J53 and K. pneumoniae K12016.

Tetracycline purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]

Time-killing assays of resistant E. coli DH5α+pAM401-tet(X4) by a combination of Tetracycline (8 μg/mL, 0-24 h) and Plumbagin.

Tetracycline purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]

Synergistic mechanisms of plumbagin in combination with tetracycline. An accelerated TCA cycle was observed after the treatment with the combination of Plumbagin and Tetracycline (16 and 32 μg/mL).
Adv Healthc Mater

Enhancing Vascularized Bone Regeneration Through Oscillatory Fluid Flow-Regulated Gradient Lattice Gradient Lattice Implants Porous Titanium Alloy. [Abstract]

2025 Oct 21:e02853. PMID: 41121591
Food Chem X

Machine learning prioritization of antibiotic residues in aquatic foods reveals exposure-driven genotoxic risk mediated by BCL2. [Abstract]

2026 Feb 8:34:103646. PMID: 41756597
Food Res Int

2026 Dec 1;245(Pt 3):120850. PMID: 42914275
Aging Cell

BNIP3-Dependent Mitophagy Non-Autonomously Regulates Systemic Aging via NF-κB Suppression in Drosophila. [Abstract]

2026 May;25(5):e70539. PMID: 42128879
Microb Biotechnol

Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria. [Abstract]

2021 Nov;14(6):2538-2551. PMID: 33720523
Drug Des Devel Ther

ED-71 Prevents Glucocorticoid-Induced Osteoporosis by Regulating Osteoblast Differentiation via Notch and Wnt/β-Catenin Pathways. [Abstract]

2022 Nov 15:16:3929-3946. PMID: 36411860
mBio

CD157 Confers Host Resistance to Mycobacterium tuberculosis via TLR2-CD157-PKCzeta-Induced Reactive Oxygen Species Production. [Abstract]

2019 Aug 27;10(4):e01949-19. PMID: 31455656

Tetracycline purchased from MedChemExpress. Usage Cited in: mBio. 2019 Aug 27;10(4):e01949-19. [Abstract]

CD157 deficiency impairs macrophage bactericidal capacity. Peritoneal macrophages from WT mice and Cd157 KO mice treated with sCD157 (+sCD157) (5 μg/ml) or not treated with sCD157 were infected with strain H37Ra harboring a dual-color reporter that comprises a constitutively green (Emerald) and a Tetracycline hydrochloride-inducible red (TagRFP) fluorescent protein for 3 days. Tetracycline hydrochloride (500 ng/ml) was added 24 h before flow cytometry. Macrophages were then harvested and fixed with 4% PFA, and the percentage of cells with live M. tuberculosis (MTB) (red) were determined by FACS.
mSystems

Repurposing of the antimalarial agent tafenoquine to combat MRSA. [Abstract]

2023 Dec 21;8(6):e0102623. PMID: 38047647
PLoS Pathog

Differential contribution of PBP occupancy and efflux on the effectiveness of β-lactams at their target site in clinical isolates of Neisseria gonorrhoeae. [Abstract]

2024 Dec 31;20(12):e1012783. PMID: 39739989
Toxics

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline. [Abstract]

2023 Jul 13;11(7):611. PMID: 37505576
Sci Rep

Qualitative immunoassay for the determination of tetracycline antibiotic residues in milk samples followed by a quantitative improved HPLC-DAD method. [Abstract]

2022 Aug 25;12(1):14502. PMID: 36008564
Microorganisms

Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]

2025 Apr 18;13(4):938. PMID: 40284774
Microorganisms

Antimicrobial Resistance and Genomic Characteristics of Escherichia coli Strains Isolated from the Poultry Industry in Henan Province, China. [Abstract]

2024 Mar 13;12(3):575. PMID: 38543626

Tetracycline purchased from MedChemExpress. Usage Cited in: Microorganisms. 2024 Mar 13;12(3):575. [Abstract]

Percentages of E. coli strains showing antimicrobial resistance to different antimicrobial agents. AMP, Ampicillin; CAZ, Ceftazidime; IPM, Imipenem; MRP, Meropenem; GEN, Gentamicin; OFX, Ofloxacin; SXT, Trimethoprim–sulfamethoxazole; TET, Tetracycline; TGC, Tigecycline; CL, Colistin; FFC, Florfenicol.
Aquat Toxicol

Antibacterials exert toxic effects on aquatic organisms by inhibiting respiration, inducing oxidative stress, mitochondrial dysfunction and autophagy. [Abstract]

2025 Mar:280:107284. PMID: 39951902
iScience

Dual transcriptomic analysis unraveling the immune landscape and host-pathogen interactions during Mycobacterium tuberculosis infection. [Abstract]

2025 Nov 17;28(12):114102. PMID: 41492386

Tetracycline purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 17;28(12):114102. [Abstract]

We performed dual RNA-seq on phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophages sub-populations infected with double-fluorescent-engineered H37Ra in vitro. This strain constitutively expressed Emerald (green) and expressed TagRFP (red) only upon Tetracycline hydrochloride induction, enabling discrimination of bacterial viability by fluorescence-activated cell sorting (FACS).
Saudi Pharm J

Determination of tetracycline, oxytetracycline and chlortetracycline residues in seafood products of Saudi Arabia using high performance liquid chromatography-Photo diode array detection. [Abstract]

2021 Jun;29(6):566-575. PMID: 34194263
Antimicrob Agents Chemother

Dihydrotanshinone I Is Effective against Drug-Resistant Helicobacter pylori In Vitro and In Vivo. [Abstract]

2021 Feb 17;65(3):e01921-20. PMID: 33318002
Microbiol Spectr

Montelukast treats Streptococcus pneumoniae-induced sepsis via antibacterial and anti-inflammatory activities. [Abstract]

2025 Oct 15:e0122125. PMID: 41090944
Microbiol Spectr

Killing of colistin-resistant Aeromonas hydrophila by a synthetic peptide. [Abstract]

2025 Aug 29:e0059025. PMID: 40879403
Microbiol Spectr

The Mechanism of Action of Ginkgolic Acid (15:1) against Gram-Positive Bacteria Involves Cross Talk with Iron Homeostasis. [Abstract]

2022 Feb 23;10(1):e0099121. PMID: 35019708
AMB Express

Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]

2024 Dec 24;14(1):141. PMID: 39718732

Tetracycline purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]

Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.
Pathogens

Mycoplasma bovis Invades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles. [Abstract]

2024 Nov 15;13(11):1003. PMID: 39599557
J Med Virol

2026 Jul;98(7):e71046. PMID: 42423506
J Med Virol

Hepatitis B Virus Promotes Angiogenesis in Hepatocellular Carcinoma by Increasing m6A Modification of VEGFA mRNA via IGF2BP3. [Abstract]

2025 May;97(5):e70356. PMID: 40260505
Chemistry

2026 May;8(5):65.
Water

2025 Jun 6.
Exp Cell Res

Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9. [Abstract]

2020 Aug 15;393(2):112090. PMID: 32416090
Anim Dis

Pasteurella multocida capsular: lipopolysaccharide types D:L6 and A:L3 remain to be the main epidemic genotypes of pigs in China. [Abstract]

2021;1(1):26. PMID: 34778886
Vet Microbiol

Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]

2024 May:292:110046. PMID: 38471428
Transbound Emerg Dis

2026;2026(1):e2248363. PMID: 42741805
J Chromatogr B Analyt Technol Biomed Life Sci

2026 Oct 1:1282:125247. PMID: 42607348
Future Microbiol

Synergistic drug strategy against mastitis-associated MRSA strains. [Abstract]

2025 Oct 13:1-10. PMID: 41082411
Biochem Biophys Res Commun

Chelerythrine inhibits NR2B NMDA receptor independent of PKC activity. [Abstract]

2024 Dec 20:739:150914. PMID: 39536412
Vet Res Commun

Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China. [Abstract]

2024 Dec;48(6):3859-3872. PMID: 39325108
Diagn Microbiol Infect Dis

In vitro activity of new tetracycline analogues omadacycline and eravacycline against clinical isolates of Helicobacter pylori collected in China. [Abstract]

2020 Nov;98(3):115129. PMID: 32739761
SSRN

2025 Jul 29.
SSRN

2025 Jan 2.
bioRxiv

2024 Jan 18.
Chemosphere

Sustainable production of Fe-doped MnO2 nanoparticles for accelerated tetracycline antibiotic detoxification. [Abstract]

2023 Dec:344:140353. PMID: 37797898
bioRxiv

2023 Feb 5.
Charles University

2020 Jul.
Research Square Preprint

2020 Jun.
Patent

US20200101105A1.
Chemosphere

Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]

2019 Jun:225:378-387. PMID: 30884299

Solvente y solubilidad

In Vitro:

DMSO : 116.67 mg/mL (262.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2501 mL

11.2504 mL

22.5007 mL

5 mM

0.4500 mL

2.2501 mL

4.5001 mL

10 mM

0.2250 mL

1.1250 mL

2.2501 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

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Referencias

[1]. Chopra I, et al. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance. Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60. [Content Brief]

[2]. Bowdre JH, et al. Antibiotic efficacy against Vibrio vulnificus in the mouse: superiority of tetracycline. J Pharmacol Exp Ther. 1983 Jun;225(3):595-8. [Content Brief]

[3]. Forloni G, et al. Anti-amyloidogenic activity of tetracyclines: studies in vitro. FEBS Lett. 2001 Jan 5;487(3):404-7. [Content Brief]

[4]. Dugray A, et al. Rapid generation of a tetracycline-inducible BCR-ABL defective retrovirus using a single autoregulatory retroviral cassette. Leukemia. 2001 Oct;15(10):1658-62. [Content Brief]

[5]. Ziółkowski H, et al. Comparative pharmacokinetics of chlortetracycline, tetracycline, minocycline, and tigecycline in broiler chickens. Poult Sci. 2020 Oct;99(10):4750-4757. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2501 mL	11.2504 mL	22.5007 mL	56.2518 mL
	5 mM	0.4500 mL	2.2501 mL	4.5001 mL	11.2504 mL
	10 mM	0.2250 mL	1.1250 mL	2.2501 mL	5.6252 mL
	15 mM	0.1500 mL	0.7500 mL	1.5000 mL	3.7501 mL
	20 mM	0.1125 mL	0.5625 mL	1.1250 mL	2.8126 mL
	25 mM	0.0900 mL	0.4500 mL	0.9000 mL	2.2501 mL
	30 mM	0.0750 mL	0.3750 mL	0.7500 mL	1.8751 mL
	40 mM	0.0563 mL	0.2813 mL	0.5625 mL	1.4063 mL
	50 mM	0.0450 mL	0.2250 mL	0.4500 mL	1.1250 mL
	60 mM	0.0375 mL	0.1875 mL	0.3750 mL	0.9375 mL
	80 mM	0.0281 mL	0.1406 mL	0.2813 mL	0.7031 mL
	100 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5625 mL

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Tetracycline

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Tetracycline

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