Referencia número: HY-B0905

Tilmicosin

(Synonyms: LY-177370; EL-870)

5 Cited Publications

Revisión del cliente

Based on 5 publication(s) in Google Scholar

Tilmicosin (LY-177370) is an orally active calcium channel antagonist and macrolide antibiotic with antimicrobial activity. Tilmicosin mainly acts on the 50S subunit of bacterial ribosomes, inhibiting protein synthesis. Tilmicosin is effective in the treatment of respiratory diseases in livestock such as cattle, sheep and pigs. In addition, Tilmicosin has immunomodulatory and anti-inflammatory effects.

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Pureza: 98.0%
No. CAS: 108050-54-0
Fòrmula: C₄₆H₈₀N₂O₁₃
Peso molecular:869.13

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Tilmicosin Chemical Structure

No. CAS: 108050-54-0

Tamaño

Precio

Stock

Cantidad

Sample (0.1mg)

In-stock

(In Japan)

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target^[3]

COX-2

Ver todos los productos específicos de isoformas Antibiotic :

Ver todos los productos específicos de isoformas Calcium Channel :

In Vitro

Tilmicosin (0-7 μg/mL; 16-18 h) has the MIC for 90% of Staphylococcus aureus isolates from the bovine udder of 0.78 μg/mL^[1].
Tilmicosin (0.5μg /mL; 2 h) induces apoptosis of bovine neutrophils^[2].
Tilmicosin (5-20 μg/mL; 18 h) inhibits the expression of COX-2 and iNOS in LPS (HY-D1056) treated macrophages, and affects the secretion of cytokines^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[3]

Cell Line:

LPS (HY-D1056) treated RAW264.7 cells
Concentration:

5, 10 and 20 μg/mL
Incubation Time:

18 h
Result:

Reduced the levels of COX-2 and iNOS.

In Vivo

Tilmicosin (75 mg/kg; subcutaneous injection; single dose) induces cardiotoxicity in mice^[4].
Pharmacokinetic Analysis in lung tissues of the M. gallisepticum infected chickens^[5]

Route	Dose (mg/kg)	t_1/2kel (h)	T_max (h)	C_max (μg/g)	AUC (μg·h/g)	MRT (h)	CL/F (1/h/kg)
p.o.	1	48.46	12.00	0.12	13.62	53.60	0.09
p.o.	30	50.60	12.00	6.19	464.23	50.88	0.06

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male albino mice^[4]
Dosage:

75 mg/kg
Administration:

Subcutaneous injection (s.c.); single dose
Result:

Significantly increased cardiac injury markers in serum (LDH, CK and CK-MB).
Significantly increased the cardiac MDA content and decreased cardiac GSH, SOD, CAT and TAC.

Chemical Information

No. CAS 108050-54-0
Appearance Solid
Peso molecular 869.13
Fòrmula C₄₆H₈₀N₂O₁₃
Color White to off-white
SMILES

C[C@@H]([C@@H](CC1=O)O)[C@H]([C@H](C[C@H](C(/C=C/C(C)=C/[C@H](CO[C@H](O[C@H](C)[C@@H](O)[C@H]2OC)[C@@H]2OC)[C@@H](CC)O1)=O)C)CCN(C[C@H](C)C3)C[C@H]3C)O[C@@](O[C@H](C)[C@@H](O)[C@@H]4N(C)C)([H])[C@@H]4O
Synonyms

LY-177370; EL-870
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (5)

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Pak Vet J

2026 Feb 25;46(2):473-484.
Microorganisms

Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]

2025 Apr 18;13(4):938. PMID: 40284774
Microorganisms

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions. [Abstract]

2024 Jan 25;12(2):256. PMID: 38399660
Vet Microbiol

Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]

2024 May:292:110046. PMID: 38471428
bioRxiv

2026 Mar 25.

Solvente y solubilidad

In Vitro:

1M HCl : 100 mg/mL (115.06 mM; ultrasonic and adjust pH to 1 with HCl)

DMSO : 100 mg/mL (115.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1506 mL

5.7529 mL

11.5058 mL

5 mM

0.2301 mL

1.1506 mL

2.3012 mL

10 mM

0.1151 mL

0.5753 mL

1.1506 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (2.88 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (293 KB)
SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Portuguese - PT (597 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Ziv G, et al. Tilmicosin antibacterial activity and pharmacokinetics in cows. J Vet Pharmacol Ther. 1995 Oct;18(5):340-5. [Content Brief]

[2]. Buret AG. Immuno-modulation and anti-inflammatory benefits of antibiotics: the example of tilmicosin. Can J Vet Res. 2010 Jan;74(1):1-10. [Content Brief]

[3]. Cao XY, et al. Tilmicosin and tylosin have anti-inflammatory properties via modulation of COX-2 and iNOS gene expression and production of cytokines in LPS-induced macrophages and monocytes. Int J Antimicrob Agents. 2006 May;27(5):431-8. [Content Brief]

[4]. Ibrahim AE, et al. Modulating Effects of Spirulina platensis against Tilmicosin-Induced Cardiotoxicity in Mice. Cell J. 2015 Spring;17(1):137-44. [Content Brief]

[5]. Zhang N, et al. Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens. Front Vet Sci. 2022 Sep 29;9:952599. [Content Brief]

[6]. Veenhuizen MF, et al. Analysis of reports of human exposure to Micotil 300 (tilmicosin injection). J Am Vet Med Assoc. 2006 Dec 1;229(11):1737-42. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
1M HCl / DMSO	1 mM	1.1506 mL	5.7529 mL	11.5058 mL	28.7644 mL
	5 mM	0.2301 mL	1.1506 mL	2.3012 mL	5.7529 mL
	10 mM	0.1151 mL	0.5753 mL	1.1506 mL	2.8764 mL
	15 mM	0.0767 mL	0.3835 mL	0.7671 mL	1.9176 mL
	20 mM	0.0575 mL	0.2876 mL	0.5753 mL	1.4382 mL
	25 mM	0.0460 mL	0.2301 mL	0.4602 mL	1.1506 mL
	30 mM	0.0384 mL	0.1918 mL	0.3835 mL	0.9588 mL
	40 mM	0.0288 mL	0.1438 mL	0.2876 mL	0.7191 mL
	50 mM	0.0230 mL	0.1151 mL	0.2301 mL	0.5753 mL
	60 mM	0.0192 mL	0.0959 mL	0.1918 mL	0.4794 mL
	80 mM	0.0144 mL	0.0719 mL	0.1438 mL	0.3596 mL
	100 mM	0.0115 mL	0.0575 mL	0.1151 mL	0.2876 mL

Keywords

Tilmicosin

5 Cited Publications

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Tilmicosin

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Macrocyclic Compound Library

Antibacterial Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Neuroprotective Compound Library

Calcium Channel Blocker Library

Anti-Prostate Cancer Compound Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cell Death Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Tilmicosin

Ver todos los productos específicos de isoformas Antibiotic

Ver todos los productos específicos de isoformas Calcium Channel

Actividad biológica

Chemical Information

Publications (5)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Tilmicosin

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Macrocyclic Compound Library

Antibacterial Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Neuroprotective Compound Library

Calcium Channel Blocker Library

Anti-Prostate Cancer Compound Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cell Death Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Tilmicosin

Ver todos los productos específicos de isoformas Antibiotic

Ver todos los productos específicos de isoformas Calcium Channel

Actividad biológica

Chemical Information

Publications (5)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Tilmicosin Related Classifications

Referencia número: HY-B0905